LOCUS       sequence-275919-        6330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments 
            of mouse Cd36 enhancers at Chr5:17843052_17844983.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Kuang Z, Wang Y, Li Y, Ye C, Ruhn KA, Behrendt CL, Olson EN, Hooper 
            LV
  TITLE     The intestinal microbiota programs diurnal rhythms in host 
            metabolism through histone deacetylase 3.
  JOURNAL   Science. 2019 Sep 27;365(6460):1428-1434. doi: 
            10.1126/science.aaw3134.
  PUBMED    31604271
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1428-1434"
COMMENT     Sequence Label: pGL4.24-Cd36-enhancer2
FEATURES             Location/Qualifiers
     source          1..6330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..120
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    172..293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(183..202)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     237..256
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(541..558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(712..1300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(792..811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1500..2291,2292..2360))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2465..2513
                     /note="synthetic polyadenylation signal"
     misc_feature    2527..2618
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2567..2586
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        4614..4645
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      4672..4681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4678..6327
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 tctcacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc catgagctgc
       61 gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag gatcaacgtc
      121 taaggccgcg actctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
      181 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      241 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      301 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
      361 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
      421 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
      481 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
      541 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      601 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      781 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      841 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      901 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      961 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1021 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1081 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1141 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1201 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1261 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1441 aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt
     1501 taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata
     1561 gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc
     1621 agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac
     1681 cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag
     1741 tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga
     1801 gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc
     1861 aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca
     1921 gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc
     1981 atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc
     2041 gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc
     2101 tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc
     2161 atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc
     2221 agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc
     2281 gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca
     2341 cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt
     2401 tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg
     2461 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     2521 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     2581 tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc cggtaccgtg
     2641 agagccaacc ccagtagtgc acatttgtaa tctgaacact agagaataaa agaataagat
     2701 aggcagattc tttctgcttt ctgtatagcc atcctagaag aatccatgag ctctaggttc
     2761 aagaagaaac ccttgctggg cagtggtggc gcacgccttt aatcccagca cttgggaggc
     2821 agaggcaggc agatatctga gttcgaggcc agcctggtct acagagtgac atgtatgaag
     2881 tcaagagagg aagcatgaga atatgcatca gattgtctgc agttttggcg gagacacatt
     2941 tcactctatt gagatgtggt gagctctcac cggaagcagc aaagcatagt ataaacagcc
     3001 gctaaactga taaaatgagc atatcaatta aaggagtcaa ggaccacagg aatgtacagt
     3061 ttcttctgac ttaagaactt gtgcgcatgt atgtggactt ggaatatatt ggtttggatg
     3121 taatcccaaa ctcacatact taaaagcaga tgccatatgg aaagaaaatt aaattcaaaa
     3181 caggatctca agtactcaaa aataaacaag tacacagtga ctgaaaagca actaaggaag
     3241 gccaaaaatc taggaccatt ctaaaaacgt tttatgcaat ctatcttctg ggatgttcat
     3301 cttcatagct ttaaataggt ttgcataaaa tgtgattatt tgctgtttgt actgattcat
     3361 ttcactacag ttttttactt acttccatcc tctttcctcc aagtagtgtt attatcaagc
     3421 tttccccaag gagttgttga aaatgagagg aaaaacaggc ctagcaatat gttggagcac
     3481 atccaaaaag gtatttttag agcgtagaac acattgagga tggtcctacc agtcttatac
     3541 atagagcttg gcccttagca tatcccttcc gaacagcaac actgttgcta ataaagacag
     3601 tgttaaggag caccacaaaa tgtactctga tttcatgagc attatgctct cacccatgag
     3661 ctagaggcat tatagaacaa gaccggtttg tagaatggtt tgctatccat agctaagagg
     3721 ctgtgagctt tcagagccca aacaaacaaa gatgtgtcct gaaagtctag catgaccttt
     3781 ttactcctta tttattgcaa aggtcacaga actcgttcag tagtaaagtc atttgaaggt
     3841 gacactccac agctcataag gttccacaac ctattactgt ttacaaaagt tataaccagg
     3901 agcaaaagag aaaagttacc ttcatgagga taagagaaga tagttaatgg agttgacaga
     3961 caatggcgta aagatagggt ttatgatgcc taattttacc ccaaagagac agaaagatgt
     4021 tattttgttt ttctctgtag ctcacagggt gaaatacttg aatcactgcc atcgtgcaat
     4081 aatgggaagc aatcgttaat gattctgcta tgctgtcagg gccaggaaaa ggatgaatga
     4141 cttaaacttc tgttacccac tggttaattg tagagattcc tacgtgatca tttcctcttc
     4201 tgctccacaa gttttgaact tctttaatct actcaagtaa caacgtccac cacaaatcac
     4261 atactcacac acacacaaac atccatggga aggttcttta ttttagggtg atttggaatt
     4321 tatactctca tattcattat agactggtag cctgacattt gttactcttt tggagtctat
     4381 tgttttctga gatgtattca taaaaaaaaa atgaaataaa gagcagtggt ggcacacaat
     4441 tttaatccca gcattccaga ggcagaggca ggcagatctc tgtgagttca aggtcagcct
     4501 ggtctacaag atgagttcca agacaaccaa tgctacacag tgaaactctg tctcaaaacc
     4561 accaccaccc tcgaggatat caagatctgg cctcggcggc caagcttaga cactagaggg
     4621 tatataatgg aagctcgact tccagcttgg caatccggta ctgttggtaa agccaccatg
     4681 gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc
     4741 gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc
     4801 tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt
     4861 cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc
     4921 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
     4981 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
     5041 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
     5101 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
     5161 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
     5221 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
     5281 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
     5341 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
     5401 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
     5461 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
     5521 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
     5581 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
     5641 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
     5701 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
     5761 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
     5821 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
     5881 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
     5941 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
     6001 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
     6061 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
     6121 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
     6181 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
     6241 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
     6301 gccaagaagg gcggcaagat cgccgtgaat
//
