LOCUS       sequence-275922-        5653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase reporter fused with a 1296 bp fragment of mouse 
            Cd36 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Kuang Z, Wang Y, Li Y, Ye C, Ruhn KA, Behrendt CL, Olson EN, Hooper 
            LV
  TITLE     The intestinal microbiota programs diurnal rhythms in host 
            metabolism through histone deacetylase 3.
  JOURNAL   Science. 2019 Sep 27;365(6460):1428-1434. doi: 
            10.1126/science.aaw3134.
  PUBMED    31604271
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1428-1434"
COMMENT     Sequence Label: pGL4.11-Cd36-promoter
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      530..539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             536..2185
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2189..2308
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    2360..2481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2371..2390)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2425..2444
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2729..2746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2900..3488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2980..2999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3688..4479,4480..4548))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4653..4701
                     /note="synthetic polyadenylation signal"
     misc_feature    4715..4806
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4755..4774
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 cgttaccact acggtcatgg gttaatagac aaggtatatc agtccagcca catttgtttt
       61 gcaggagaat tcctggcaaa gcactcaggg ttctgtttgg gtggagaaac tagatagggc
      121 gtgaaggtat aaaaaagcat gccacactgt cgtaaggagt attgggatgg gaatatgttt
      181 atatggctcg ccttagataa gattatagta tgttaatcac gaagggatta atgtgcaaaa
      241 cagaaaagtt taaaaaccaa gcattctctc tgttactcct tctgcttctg gtgagttttt
      301 ttatgaacaa aaagaaggag tagaacctag aaatccagtg tggtgaaaca aacaaaaaga
      361 cttgtttgtt agttggcaaa atcatctgta tcagtagcat gatttctttg tactttgatc
      421 tttttgtgct aatacttaag cttctttttt atgacctctt tctaatagct cgaggatatc
      481 aagatctggc ctcggcggcc aagcttggca atccggtact gttggtaaag ccaccatgga
      541 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
      601 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
      661 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
      721 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
      781 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
      841 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
      901 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
      961 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
     1021 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
     1081 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     1141 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     1201 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
     1261 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     1321 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
     1381 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     1441 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     1501 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     1561 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     1621 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     1681 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     1741 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     1801 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     1861 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     1921 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     1981 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     2041 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     2101 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     2161 caagaagggc ggcaagatcg ccgtgaattc tcacggcttc cctcccgagg tggaggagca
     2221 ggccgccggc accctgccca tgagctgcgc ccaggagagc ggcatggata gacaccctgc
     2281 tgcttgcgcc agcgccagga tcaacgtcta aggccgcgac tctagagtcg gggcggccgg
     2341 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     2401 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2461 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2521 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     2581 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     2641 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     2701 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2761 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2821 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2881 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2941 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3001 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3061 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3121 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3181 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3241 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3301 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3361 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3421 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3481 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3541 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3601 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     3661 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     3721 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     3781 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     3841 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     3901 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     3961 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     4021 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     4081 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     4141 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     4201 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     4261 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     4321 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     4381 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     4441 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     4501 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     4561 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     4621 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     4681 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     4741 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     4801 ttctctggcc taactggccg gtaccagaaa ggcttatggt tgtactcaga gtattttata
     4861 aatgctctct gtgtgcctta tcttttcttg gttctattgg catctgtgta gcgctcttgg
     4921 ctatgggcat tttgatgtta cacaaaactg ttctaaagta caaaccacag gtatacacca
     4981 caccatgaag ggttaaacac catcataaaa ctgtcactga agcccaactt cttcttcaag
     5041 tattgatcaa aaagacacga ttctatcagt tttgttacag caggatcctg ttgttctgta
     5101 aattgcttct ttccaatgtt atataatacc cacacaaaat aagatattgc aaattaaact
     5161 gcctgcatgg agtgcatagt agcaatacta ctgtatttaa gagcattgac atgaatctct
     5221 acataggaaa aaaataatgc tactagaatt ggatacacaa gggaatcttt aagaataaca
     5281 aaaaaccaac tataatatat acatattata tttgtatctt caaattcttt agcatatgtt
     5341 ttgtcaaaat cattgataat gaaatcagaa accatatact tttaaaagtt tctggaatgt
     5401 tttccacact gtatggggaa agtttcaggc atgaacataa aaactggaaa ggaagtggaa
     5461 ttttcattaa tgctatgatt atattaaaga aaatagtagt attgattttg tgataatgtt
     5521 ttttcctctg catttttttt gttgttgttt tgacaggtag tttacaaatg atttgaagtg
     5581 ctgatccttt cagagtctca acagattatt tcttaaacag ctcatacatt gctgtttatg
     5641 catgaattag gta
//
