LOCUS       sequence-275923-       12166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the CeNLuc LumiFluor in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Parag-Sharma K, O'Banion CP, Henry EC, Musicant AM, Cleveland JL, 
            Lawrence DS, Amelio AL
  TITLE     Engineered BRET-Based Biologic Light Sources Enable Spatio-Temporal 
            Control Over Diverse Optogenetic Systems.
  JOURNAL   ACS Synth Biol. 2019 Dec 13. doi: 10.1021/acssynbio.9b00277.
  PUBMED    31834783
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Dec 13. doi: 10.1021/acssynbio.9b00277."
COMMENT     Sequence Label: pWXLd-RIEP-CeNLuc
FEATURES             Location/Qualifiers
     source          1..12166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2154..3332
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2385..3323
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3280..3300
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3380..3497
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(3540..3542,3543..3545,3546..4256)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3585..3606)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3846..3865)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4193..4214
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4272..4787
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     promoter        4798..5297
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4833..4856)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5230..5249
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5318..5917
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5318..5337)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5814..5834
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(5987..6020)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6051..6639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6104..6124)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6522..6533)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6728..6961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6986..7004)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6987..7004)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7294..7313)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7527..7546)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7646..7668
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7706..7724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7792..7896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7897..7965,7966..8757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8115..8134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8928..9516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9417..9436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9670..9687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9757..9777)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9762..10091
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9942..10077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11225..11290
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11420..11440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11865..11999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11902..11921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11956..11975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgt cgaacgcgtt cgacgataag ctttgcaaag atggataaag ttttaaacag
     2101 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     2161 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2221 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2281 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2341 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     2401 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     2461 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     2521 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     2581 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     2641 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     2701 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     2761 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     2821 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     2881 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     2941 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     3001 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     3061 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3121 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3181 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3241 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3301 aaagtttttt tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaat
     3361 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     3421 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     3481 caaaaattca aaattttatc gatcacgaga ctagcctcga ggtttaaact acgggatcca
     3541 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3601 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3661 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3721 tcgtgaccac cctgagctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     3781 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3841 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3901 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3961 agctggagta caacgccatc cacggcaacg tctatatcac cgccgacaag cagaagaacg
     4021 gcatcaaggc caacttcggc ctcaactgca acatcgagga cggcagcgtg cagctcgccg
     4081 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4141 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4201 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggata
     4261 tctccggagg tatggtcttc acactcgaag atttcgttgg ggactggcga cagacagccg
     4321 gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg
     4381 gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg
     4441 acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa
     4501 aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg
     4561 gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg
     4621 aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca
     4681 acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca
     4741 tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcgtaagaa ttctaccggg
     4801 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     4861 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     4921 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     4981 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     5041 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     5101 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     5161 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     5221 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     5281 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     5341 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5401 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5461 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5521 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5581 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5641 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5701 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5761 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5821 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5881 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     5941 cccgaccgaa aggagcgcac gaccccatgc aggactagtg gtgaccataa cttcgtatag
     6001 tatacattat acgaagttat ggtgaccatt gcattggcta gtcatatgat aatcaacctc
     6061 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6121 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6181 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6241 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6301 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6361 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6421 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6481 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6541 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6601 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcggtacgta tggccaggta
     6661 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6721 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6781 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6841 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6901 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6961 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     7021 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     7081 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     7141 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     7201 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     7261 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     7321 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     7381 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     7441 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     7501 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     7561 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     7621 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     7681 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     7741 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7801 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7861 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7921 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7981 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8041 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8101 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8161 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8221 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8281 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8341 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8401 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8461 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8521 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8581 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8641 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8701 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8761 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8821 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8881 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8941 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9001 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9061 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9121 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9181 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9241 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9301 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9361 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9421 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9481 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9541 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9601 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9661 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9721 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     9781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9841 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9901 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     9961 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10021 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10081 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
    10141 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
    10201 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
    10261 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
    10321 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
    10381 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
    10441 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
    10501 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
    10561 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
    10621 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
    10681 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
    10741 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
    10801 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
    10861 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
    10921 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
    10981 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
    11041 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
    11101 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
    11161 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
    11221 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
    11281 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
    11341 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
    11401 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
    11461 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
    11521 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
    11581 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
    11641 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
    11701 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
    11761 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    11821 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    11881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    11941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    12001 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    12061 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    12121 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
