LOCUS       sequence-275927-       13501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep63 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13501)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep63
FEATURES             Location/Qualifiers
     source          1..13501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5069..5087)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5155..5259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5260..5328,5329..6120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5478..5497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6291..6879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6780..6799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7068..7088)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7086..7383
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7460..8059
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7460..7479)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7956..7976
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8150..8284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8422..8441)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8718..8979
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9096..9668
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9676..9695
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9723..9742
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9723..9741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9752..10372
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9825..9844)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10414..10535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10590..10969
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10970..11173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11124..11144
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11175..11193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11196..11214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11224..11248
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11348..11371
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11372..11389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11939..11962
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11975..11999
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             12217..12225
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(join(13491..13501,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     3841 ctcctgctcc tgcccctcct scccctcctg ctcctgcccc tcctgcccct cctgctccts
     3901 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     3961 ctgcccctcc tcctgctcct gcccctcctg ctcctkcccc tcctgcccct cctgcccctc
     4021 ctgctcctgc ccctcctcct gctcctcccc cycctscycc yccyscccct cctcctgctc
     4081 ctgcccctcc tgcccctcct cctsctcctg cccctcctcc tgctcctscc cctcctgccc
     4141 ctcctgcccc tcctcctgct cctscccctc ctgcccctcc tcctgcycct kccccyacct
     4201 cctsctccys cccctccwsc ccctcctscc cctcctcctg ctcctgcccc tcctcctgct
     4261 cctgcccctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     4321 gctcctgccc ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg
     4381 gtccctttgc agccaatgca acttggacgt ttttggggtc tccggacacc atctctatgt
     4441 cttggccctg atcctgagcc gcccggggct cctggtcttc cgcctcctcg tcctcgtcct
     4501 cttccccgtc ctcgtccatg gttatcaccc cctcttcttt gaggtccact gccgccggag
     4561 ccttctggtc cagatgtgtc tcccttctct cctaggccat ttccaggtcc tgtacctggc
     4621 ccctcgtcag acatgattca cactaaaaga gatcaataga catctttatt agacgacgct
     4681 cagtgaatac agggagtgca gactcctgcc ccctccaaca gcccccccac cctcatcccc
     4741 ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc ccatcctccg aaccatcctc
     4801 gtcctcatca ccaattactc gcagcccgga aaactcccgc tgaacatcct caagatttgc
     4861 gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc tttttgctgg acggtaggga
     4921 tggggattct cgggacccct cctcttcctc ttcaaggtca ccagacagag atgctactgg
     4981 ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag cttatcgatg ataagctgtc
     5041 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5101 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5221 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5281 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5341 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5401 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5461 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5521 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5581 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5641 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5701 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5761 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     5821 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5881 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5941 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6001 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6061 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6421 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6901 ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc atctacctgc
     6961 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
     7021 atggggaagg ccatccagcc tcgcgtcgaa ctagatgatc cggctgtgga atgtgtgtca
     7081 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7141 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7201 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7321 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7381 tggagggtga ccgccacgac cggtgccgcc accatcccct gacccacgcc cctgacccct
     7441 cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     7501 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     7561 ccacaccgtc gaccccgacc gccacatcga acgcgtcacc gagctgcaag aactcttcct
     7621 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     7681 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     7741 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     7801 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7861 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7921 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7981 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8041 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     8101 acgacccggt ccgacgcggc ccgacgggtc cgaggggggt cgacctcgaa acttgtttat
     8161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8281 gatctgatcc gaaccccttc ctcgaccaat tctcatgttt gacagcttat catcgcagat
     8341 ccgggcaacg ttgttgccat tgctcgacca attctcatgt ttgacagctt atcatcgcag
     8401 atccgtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     8461 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     8521 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     8581 gcttcgcgat gtacgggcca gatatacgcg tatctgaggg gactagggtg tgtttaggcg
     8641 aaaagcgggg cttcggttgt acgcggttag gagtcccctc aggatatagt agtttcgctt
     8701 ttgcataggg agggggaaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     8761 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     8821 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     8881 ggacgaacca ctgaattccg cattgcagag atattgtatt taagtgccta gctcgataca
     8941 ataaacgcca tttgaccatt caccacattg gtgtgcacct ccaagctggc cggggatcct
     9001 ctagggcctc tgagctattc cagaagtagt gaagaggctt ttttggaggc ctaggctttt
     9061 gcaaaaagct ccggatcgat cctgagaact tcagggtgag tttggggacc cttgattgtt
     9121 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
     9181 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
     9241 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
     9301 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
     9361 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
     9421 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     9481 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc
     9541 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     9601 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt
     9661 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     9721 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat
     9781 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     9841 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     9901 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     9961 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
    10021 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
    10081 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
    10141 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
    10201 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
    10261 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
    10321 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
    10381 cggatcccgg gaattcagat cttattaaag cagaacttgt ttattgcagc ttataatggt
    10441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10501 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggtcgaa tccatggcgc
    10561 agaactggta ggtatggaag atccgcgttg acattgatta ttgactagtt attaatagta
    10621 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    10681 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    10741 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    10801 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
    10861 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
    10921 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
    10981 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
    11041 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
    11101 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
    11161 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
    11221 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
    11281 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttac
    11341 caccatggat tataaagacg atgatgacaa gcaccaccac caccaccacg acaaggattg
    11401 tgaaatgaaa cgcaccacac tggacagccc tttggggaag ctggagctgt ctggttgtga
    11461 gcagggtctg cacgaaataa tttttctggg caaggggacg tctgcagctg atgccgtgga
    11521 ggtcccagcc cccgctgcgg ttctcggagg tccggagccc ctgatgcagg ctacagcctg
    11581 gctgaatgcc tatttccacc agcccgaggc tatcgaagag ttccccgtgc cggctcttca
    11641 ccatcccgtt ttccagcaag agtcgttcac cagacaggtg ttatggaagc tgctgaaggt
    11701 tgtgaaattc ggagaagtga tttcttactc tcacttagca gccctggcag gcaaccccgc
    11761 agccacggca gcagtgaaga cggcactgag tggcaatcct gtccctatcc tgatcccgtg
    11821 ccacagagtg gtccaggggg atttggatgt gggcggttac gagggtggac tggccgtgaa
    11881 ggaatggctt ctggcccatg aaggccaccg gttggggaag ccaggcttgg gattcgaact
    11941 ggaggtgctg ttccagggcc ccggtaccgc catcacaagt ttgtacaaaa aagcaggcac
    12001 catggaggct ttgttagaag gaatacaaaa tcgagggcat ggtgggggat ttttgacatc
    12061 ttgtgaagca gaactacagg agctcatgaa acagattgac ataatggtgg ctcataaaaa
    12121 atctgaatgg gaaggacgta cacatgctct agaaacttgc ttgaaaatcc gtgaacagga
    12181 acttaagagt cttaggagtc agttggatgt gacacataag gaggttggaa tgttgcatca
    12241 gcaggtagaa gaacatgaaa aaatcaagca agagatgacc atggaatata agcaggagtt
    12301 gaagaaacta catgaagaat tatgcatact gaagagaagc tatgaaaagc ttcagaaaaa
    12361 gcaaatgagg gaattcagag gaaataccaa aaatcacagg gaagatcggt ctgaaattga
    12421 gaggttaact gcaaaaatag aggaattccg tcagaaatcg ctggactggg agaagcaacg
    12481 cttgatttat cagcaacagg tatcttcact ggaggcacaa aggaaggctc tggctgaaca
    12541 atcagagata attcaggctc agcttgtcaa tcggaaacag aaattagagt ctgtggaact
    12601 ttctagccaa tcagaaattc aacacttaag cagtaaactg gagcgggcta atgacactat
    12661 ctgtgccaat gagttggaaa tagagcgcct caccatgagg gtcaatgact tggttggaac
    12721 cagtatgact gtcctacagg agcagcagca aaaagaagaa aaattgaggg aatctgaaaa
    12781 actattagag gctctgcagg aagaaaagag agaattgaag gcagctcttc agtctcaaga
    12841 aaatctcata catgaggcca gaatacaaaa ggagaagtta caagaaaaag taaaggcaac
    12901 taacactcaa catgctgtag aagctataag tttggaatct gtgagtgcaa cgtgtaaaca
    12961 gctgagccaa gaactaatgg aaaaatatga agaactgaag aggatggaag cacataacaa
    13021 tgaatacaaa gcagagatta agaagttgaa agaacagatt ttacagggtg aacaaagtta
    13081 cagttctgca ctagaaggaa tgaagatgga aatctcccat ctaactcagg agttacatca
    13141 gcgagatatc actattgctt ccaccaaagg ttcttcctca gacatggaaa agcgactcag
    13201 agcagagatg caaaaggcag aagacaaagc agtagagcat aaggagattt tggatcagct
    13261 ggagtcactc aaattagaaa atcgtcatct ttctgaaatg gtgatgaaat tggaattggg
    13321 tttacatgag tgttccttgc ctgtatctcc ccttggttca atagctacca gatttttgga
    13381 agaggaggaa ctgaggtctc atcacattct agagcgcttg gatgcccata ttgaagaact
    13441 aaaaagagag agtgaaaaga cagtgagaca attcacagcc ttaaagtaga acccagcttt
    13501 c
//
