LOCUS       sequence-275929-        6299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments 
            of mouse Cd36 enhancers at Chr5:17848003_17849903.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Kuang Z, Wang Y, Li Y, Ye C, Ruhn KA, Behrendt CL, Olson EN, Hooper 
            LV
  TITLE     The intestinal microbiota programs diurnal rhythms in host 
            metabolism through histone deacetylase 3.
  JOURNAL   Science. 2019 Sep 27;365(6460):1428-1434. doi: 
            10.1126/science.aaw3134.
  PUBMED    31604271
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1428-1434"
COMMENT     Sequence Label: pGL4.24-Cd36-enhancer3
FEATURES             Location/Qualifiers
     source          1..6299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..32
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..1714
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1718..1837
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1889..2010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1900..1919)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1954..1973
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2258..2275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2429..3017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2509..2528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3217..4008,4009..4077))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4182..4230
                     /note="synthetic polyadenylation signal"
     misc_feature    4244..4335
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4284..4303
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc
       61 caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga
      121 cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac
      181 catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat
      241 gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt
      301 ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg
      361 tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg
      421 catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt
      481 gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca
      541 gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta
      601 cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag
      661 tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt
      721 cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag
      781 cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg
      841 ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga
      901 ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac
      961 tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct
     1021 cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg
     1081 ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc
     1141 tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg
     1201 taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag
     1261 cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca
     1321 cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa
     1381 gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct
     1441 gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga
     1501 gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt
     1561 ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt
     1621 ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct
     1681 cattaaggcc aagaagggcg gcaagatcgc cgtgaattct cacggcttcc ctcccgaggt
     1741 ggaggagcag gccgccggca ccctgcccat gagctgcgcc caggagagcg gcatggatag
     1801 acaccctgct gcttgcgcca gcgccaggat caacgtctaa ggccgcgact ctagagtcgg
     1861 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     1921 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1981 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2041 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2101 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     2161 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     2221 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2281 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2341 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2401 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2461 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2521 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2581 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2641 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2701 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2761 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2821 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2881 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2941 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3001 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3061 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3181 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     3241 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     3301 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     3361 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     3421 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     3481 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     3541 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     3601 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     3661 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     3721 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     3781 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     3841 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     3901 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     3961 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     4021 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     4081 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     4141 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     4201 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     4261 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     4321 ccagaacatt tctctggcct aactggccgg taccatgctg gatgccaggt ttactttggt
     4381 tcagaaactt cactgctgca ctagagtgtc aatcaaaatt ccacagccat tctaggacac
     4441 ttggaggtgg ggctaagtgg tgagcagcag tgatttgact tgtggcaaac agggctggag
     4501 atatcaggga ctctgtaacc ttgcaactgc attttgatgg ttcctttaaa cttgtggcct
     4561 tgcactctct catcggactt ctagctgatt acttctgtgt agtaagtaaa acttaactag
     4621 acaaaaattt ggtgatccag acttgtgctt gcaaatctaa aatcagttct tttgatagga
     4681 ttatttgatt tgttcttgta cagtttagag aataattggg attttctcac agaaaggggg
     4741 ataagtggga agtattatat gtattcagtt atcctgctta aatctgtaat tgagaaggaa
     4801 agtagacact ttgtcagcta tactaattat actctaattt tgacagtgat gtttaggtta
     4861 ctaagcgatt aaaaaggatt cagaaatatt taaaattata ataccatata tatgtgtata
     4921 tctatctatc tatctatcta tctatctatc tatctatcta tctatttatc tatattcata
     4981 tatgttgtat tagaggcata tcttatctta atggggttaa atacatctct attttctgtc
     5041 tatctgtcta tctatctgtc tgtctctcta tctgcctatc tatctgtcta tctgcctgtt
     5101 tatctaccta tctgcctatc tatttatgta tcccaagaag actgttaaac agcggatagt
     5161 cttttttttt ttctaaagaa ggaatcataa taaacattat aaccaaggct ttagcctagg
     5221 aagattttgc agcagagtgt ttgtctttga aatctttcaa aaagaagact ataaccttgc
     5281 ctgtttctgg tgaccgatta taataaacag aggtcacagg cagtccttgc tactggcttt
     5341 cctggcatgg tgcaagtcaa caggtcctct gggaaatatt tgtaaggtga ttttgcaact
     5401 ttgtcttttt tttctcaagt taaaaatttc atcactgtga tatgtaactc atttaacata
     5461 agctactgaa acataatgaa atagttaata gggtaataag aaatactaaa atcctaaagt
     5521 taaattaaaa agtgttcaat ccacaaatta ttaccaaggt gttttcatgg taataatttg
     5581 ctgataatcg aaggtatacg aaatcaggta ttgaaattaa gcaaagttgt aggcacaatt
     5641 gctttattgg ctatatataa tacttctact actcagtgct ttatgttttg acttacattg
     5701 tgatgcaatg ctctatagca atatttgaac agcttgactg tagttacatt acagggttgg
     5761 caatattgcc tagggaatta gaccatgggg caacaggcat acttagcctc agtgggcact
     5821 gggagaatgc agggttgaat gtctttctag attggaggag ataatgctaa cacatatttc
     5881 cccccaaatc aaagctgtgc ttgtgtctag atctgtccaa ttctctttct cccttgggca
     5941 actagaagtg gtacttacat attgtaaact atactttcac aatctcaggt aatttctact
     6001 tcatataatc tagcttacct aaagctttaa atactaacta caaaccagtg ttgtattgtg
     6061 tagtaatgct acaaaacata ttttatatta agtgacaatg aactcattaa ctttagaatg
     6121 atataaataa ctatctctga agagctagat gttgtgtttt atcttgtttg ttgaatggat
     6181 atgaaatact gattattttc taaacttaat tttatgcact tagcactgta aacattcaaa
     6241 gaattcccag tgcaactcga ggatatcaag atctggcctc ggcggccaag cttagacac
//
