LOCUS       sequence-27593-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK
  TITLE     Oligomerized pool engineering (OPEN): an 'open-source' protocol for 
            making customized zinc-finger arrays.
  JOURNAL   Nat Protoc. 2009. 4(10):1471-501.
  PUBMED    19798082
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2009. 4(10):1471-501."
COMMENT     Sequence Label: pKJ1267 (aka pAC-alphaGal4)
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacggattca ctggaactct agaccaaaga
       61 gaggacacaa tgcagggttc tgtgacagag tttctaaaac cgcgcctggt tgatatcgag
      121 caagtgagtt cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg ctttggccat
      181 actctgggta acgcactgcg ccgtattctg ctctcatcga tgccgggttg cgcggtgacc
      241 gaggttgaga ttgatggtgt actacatgag tacagcacca aagaaggcgt tcaggaagat
      301 atcctggaaa tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg caaagatgaa
      361 gttattctta ccttgaataa atctggcatt ggccctgtga ctgcagccga tatcacccac
      421 gacggtgatg tcgaaatcgt caagccgcag cacgtgatct gccacctgac cgatgagaac
      481 gcgtctatta gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc
      541 cgaattcatt cggaagaaga tgagcgccca atcggccgtc tgctggtcga cgcatgctac
      601 agccctgtgg agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac
      661 ctggacaagc tggtcatcga aatggaaacc aacggcacaa tcgatcctga agaggcgatt
      721 cgtcgtgcgg caaccattct ggctgaacaa ctggaagctt tcgttgactt acgtgatgta
      781 cgtcagcctg aagtgaaaga agagaaacca ga
//
