LOCUS       sequence-275934-       13996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep76 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13996)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep76
FEATURES             Location/Qualifiers
     source          1..13996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5072..5090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5158..5262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5263..5331,5332..6123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5481..5500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6294..6882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6783..6802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7071..7091)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7089..7386
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7463..8062
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7463..7482)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7959..7979
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8153..8287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8425..8444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8721..8982
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9099..9671
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9679..9698
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9726..9745
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9726..9744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9755..10375
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9828..9847)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10417..10538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10593..10972
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10973..11176
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11127..11147
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11178..11196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11199..11217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11227..11251
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11351..11374
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11375..11392
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11942..11965
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11978..12002
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13986..13996,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctmctg cccctcctcc tcctcctgcy cctcctgcyc
     3841 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     3901 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctmccgcc ycctccccct
     3961 nnccctkccc ctcctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc
     4021 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
     4081 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     4141 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     4201 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     4261 gctcctgccc ctcctscccc tcctscccct cctgcccctc ctcctgctcc tgcccctcct
     4321 cctgctcctg cccctcctgc tcctgcccct cccgctcctg ctcctgctcc tgttccaccg
     4381 tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac accatctcta
     4441 tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt
     4501 cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc actgccgccg
     4561 gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct
     4621 ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac
     4681 gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc
     4741 cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc
     4801 ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt
     4861 tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag
     4921 ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac
     4981 tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct
     5041 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5101 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5161 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5221 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5281 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5341 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5401 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5461 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     5521 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5581 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5641 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5701 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5761 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     5821 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5881 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5941 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6001 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6061 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6121 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6181 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6241 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6361 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6421 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6481 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6541 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6601 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6661 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6721 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6781 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6841 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6901 tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc
     6961 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     7021 ataatgggga aggccatcca gcctcgcgtc gaactagatg atccggctgt ggaatgtgtg
     7081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7381 ttttggaggg tgaccgccac gaccggtgcc gccaccatcc cctgacccac gcccctgacc
     7441 cctcacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7501 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7561 gcgccacacc gtcgaccccg accgccacat cgaacgcgtc accgagctgc aagaactctt
     7621 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7681 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7741 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7801 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     7861 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7921 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     7981 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8041 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     8101 cgcacgaccc ggtccgacgc ggcccgacgg gtccgagggg ggtcgacctc gaaacttgtt
     8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8281 ctggatctga tccgaacccc ttcctcgacc aattctcatg tttgacagct tatcatcgca
     8341 gatccgggca acgttgttgc cattgctcga ccaattctca tgtttgacag cttatcatcg
     8401 cagatccgta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8461 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8521 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     8581 gctgcttcgc gatgtacggg ccagatatac gcgtatctga ggggactagg gtgtgtttag
     8641 gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc ctcaggatat agtagtttcg
     8701 cttttgcata gggaggggga aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     8761 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     8821 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     8881 attggacgaa ccactgaatt ccgcattgca gagatattgt atttaagtgc ctagctcgat
     8941 acaataaacg ccatttgacc attcaccaca ttggtgtgca cctccaagct ggccggggat
     9001 cctctagggc ctctgagcta ttccagaagt agtgaagagg cttttttgga ggcctaggct
     9061 tttgcaaaaa gctccggatc gatcctgaga acttcagggt gagtttgggg acccttgatt
     9121 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     9181 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     9241 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     9301 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     9361 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     9421 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     9481 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     9541 tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     9601 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     9661 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     9721 aattgtaata cgactcacta tagggcgaat tgatatgtct agattagata aaagtaaagt
     9781 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
     9841 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
     9901 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
     9961 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
    10021 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
    10081 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
    10141 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
    10201 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
    10261 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
    10321 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgcgta
    10381 cagcggatcc cgggaattca gatcttatta aagcagaact tgtttattgc agcttataat
    10441 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10501 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggtc gaatccatgg
    10561 cgcagaactg gtaggtatgg aagatccgcg ttgacattga ttattgacta gttattaata
    10621 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
    10681 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    10741 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    10801 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    10861 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    10921 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    10981 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    11041 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    11101 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    11161 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
    11221 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
    11281 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
    11341 taccaccatg gattataaag acgatgatga caagcaccac caccaccacc acgacaagga
    11401 ttgtgaaatg aaacgcacca cactggacag ccctttgggg aagctggagc tgtctggttg
    11461 tgagcagggt ctgcacgaaa taatttttct gggcaagggg acgtctgcag ctgatgccgt
    11521 ggaggtccca gcccccgctg cggttctcgg aggtccggag cccctgatgc aggctacagc
    11581 ctggctgaat gcctatttcc accagcccga ggctatcgaa gagttccccg tgccggctct
    11641 tcaccatccc gttttccagc aagagtcgtt caccagacag gtgttatgga agctgctgaa
    11701 ggttgtgaaa ttcggagaag tgatttctta ctctcactta gcagccctgg caggcaaccc
    11761 cgcagccacg gcagcagtga agacggcact gagtggcaat cctgtcccta tcctgatccc
    11821 gtgccacaga gtggtccagg gggatttgga tgtgggcggt tacgagggtg gactggccgt
    11881 gaaggaatgg cttctggccc atgaaggcca ccggttgggg aagccaggct tgggattcga
    11941 actggaggtg ctgttccagg gccccggtac cgccatcaca agtttgtaca aaaaagcagg
    12001 caccatgtcg ctgcctccgg agaaagcctc cgagctgaag cagctcatcc accagcagct
    12061 gagcaagatg gatgtccatg gtagaataag agaaatcctt gctgagacta tacgggaaga
    12121 attggcacct gatcaacagc atttatcaac agaagatttg atcaaagccc ttagacgtcg
    12181 aggaatcatt gacgatgtga tgaaagaact taattttgtt actgacagtg ttgagcaaga
    12241 actcccttcc tctccaaaac aacctatttg ttttgataga caatcgacat taaaaaaaac
    12301 taatattgat ccaacacgga ggtatcttta ccttcaggtt ttgggtggaa aagctttctt
    12361 ggaacatctg caagaacctg agcctttacc tggacaagtt tgttcaacgt ttactttatg
    12421 tttacattat cgaaaccaac gttttcgttc taaacctgtt ccatgtgcct gtgaaccaga
    12481 ttttcatgat ggctttttac ttgaagtaca cagagaaagc ttgggtgatg gaactagaat
    12541 ggctgattca acaacaatgt tatcaataag tgatccaatt catatggtgc taatcaaaac
    12601 agacatattt ggtgagacga ctttagtagc atcatatttt ctggaatggc gatcggtttt
    12661 gggctcagaa aatggagtga ccagtctgac tgtggaactt atgggtgtag gcacagaatc
    12721 aaaagtttct gtgggaattt taaatataaa acttgaaatg tatccaccac tcaatcaaac
    12781 gttatctcaa gaagtagtga acacacagct tgctttggaa cgtcagaaaa ctgcagagaa
    12841 agagcgatta tttcttgtat atgctaagca gtggtggaga gaatatttgc aaattcgacc
    12901 ctcacacaac tcacgactgg ttaagatttt tgcacaggat gaaaatggga taaatagacc
    12961 agtctgttcc tatgttaaac cacttcgagc tggacggctt cttgatactc caaggcaagc
    13021 agcaagattt gttaatgtcc ttggttatga acgagcccct gttattggag gaggaggtaa
    13081 acaggagcag tggtgcactc tgctggcctt tctctgtaga aacaagggtg actgtgaaga
    13141 tcacgctaac cttctgtgca gccttcttct tggatatgga ttagaagcct ttgtttgtgt
    13201 tgggaccaag gcaaaaggag tacctcatgc atgggttatg acttgtggaa ctgatggggc
    13261 catcactttt tgggagagtt taacaggaca caggtacatc cataaaccta ccaatcctga
    13321 tgaacctcca gttgctgaac agcccaaacc actgtaccca tatcgaacaa ttggttgtgt
    13381 tttcaaccat cagatgttcc tgggaaattg tcaaccctct gatgcagtag aaacctgtgt
    13441 atttgatttg aacgatgaat ccaaatggaa acccatgagt gaggaagcaa ttaaatctgt
    13501 gtgtgctcct ggagctacaa catcccttcc tccctttcca cctctgtgtg catccacaat
    13561 tgacgcgtca gtaacaagta atgaaattga aatgcagctg aggctcctgg tgtcagaaca
    13621 caggaaggat cttggcctca ctactgtttg ggaagaccag ctctcctacc ttttatcacc
    13681 agctttggct tcttatgaat ttgagcgtac aacaagtata tcagcaggca atgaagaatt
    13741 tcaagatgcc ataagaaggg ctgtacctga tggtcacaca tttaaagggt tcccaataca
    13801 ttttgtgtat agaaatgcaa gacgtgcatt tgccacatgt cttcgatctc ctttctgtga
    13861 agaaataatc tgttgccgtg gagaccaagt gcgactggca gttcgtgtcc gagtatttac
    13921 ttaccctgaa tctgcatgtg ctgtttggat catgtttgct tgtaaatatc gctcggtatt
    13981 atagaaccca gctttc
//
