LOCUS       sequence-275943-       12157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the OgNLuc LumiFluor in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Parag-Sharma K, O'Banion CP, Henry EC, Musicant AM, Cleveland JL, 
            Lawrence DS, Amelio AL
  TITLE     Engineered BRET-Based Biologic Light Sources Enable Spatio-Temporal 
            Control Over Diverse Optogenetic Systems.
  JOURNAL   ACS Synth Biol. 2019 Dec 13. doi: 10.1021/acssynbio.9b00277.
  PUBMED    31834783
REFERENCE   2  (bases 1 to 12157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Dec 13. doi: 10.1021/acssynbio.9b00277."
COMMENT     Sequence Label: pWXLd-RIEP-OgNLuc
FEATURES             Location/Qualifiers
     source          1..12157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2154..3332
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2385..3323
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3280..3300
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3380..3497
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3540..4247
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3953..3972
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4263..4778
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     promoter        4789..5288
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4824..4847)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5221..5240
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5309..5908
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5309..5328)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5805..5825
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(5978..6011)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6042..6630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6095..6115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6513..6524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6719..6952
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6977..6995)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6978..6995)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7285..7304)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7518..7537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7637..7659
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7697..7715)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7783..7887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7888..7956,7957..8748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8106..8125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8919..9507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9408..9427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9661..9678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9748..9768)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9753..10082
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9933..10068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11216..11281
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11411..11431
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11856..11990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11893..11912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11947..11966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgt cgaacgcgtt cgacgataag ctttgcaaag atggataaag ttttaaacag
     2101 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     2161 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2221 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2281 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2341 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     2401 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     2461 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     2521 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     2581 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     2641 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     2701 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     2761 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     2821 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     2881 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     2941 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     3001 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     3061 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3121 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3181 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3241 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3301 aaagtttttt tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaat
     3361 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     3421 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     3481 caaaaattca aaattttatc gatcacgaga ctagcctcga ggtttaaact acgggatcca
     3541 tggtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     3601 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     3661 gcccctacga gggctttcag accgttaagc tgaaggtgac caagggtggc cccctgccct
     3721 tcgcctggga catcttgtcc cctcagttca cctacggctc caaggcctac gtgaagcacc
     3781 ccgccgacat ccccgactac ctcaagctgt ccttccccga gggcttcaag tgggagcgcg
     3841 tgatgaactt cgaggacggc ggcgtggtga ccgtgactca ggactcctcc ctgcaggacg
     3901 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     3961 tgcagaagaa gaccatgggc atggaggcct cctccgagcg gatgtacccc gaggacggcg
     4021 ccctgaaggg cgaggacaag ctcaggctga agctgaagga cggcggccac tacacctccg
     4081 aggtcaagac cacctacaag gccaagaagc ccgtgcagtt gcccggcgcc tacatcgtcg
     4141 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     4201 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggat atctccggag
     4261 gtatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc ggctacaacc
     4321 tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg
     4381 taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg
     4441 tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa aaaattttta
     4501 aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat ggcacactgg
     4561 taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat gaaggcatcg
     4621 ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc aacaaaatta
     4681 tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc atcaacggag
     4741 tgaccggctg gcggctgtgc gaacgcattc tggcgtaaga attctaccgg gtaggggagg
     4801 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     4861 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     4921 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     4981 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     5041 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     5101 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     5161 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     5221 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     5281 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     5341 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5401 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     5461 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     5521 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     5581 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     5641 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     5701 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     5761 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     5821 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     5881 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     5941 aaggagcgca cgaccccatg caggactagt ggtgaccata acttcgtata gtatacatta
     6001 tacgaagtta tggtgaccat tgcattggct agtcatatga taatcaacct ctggattaca
     6061 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6121 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6181 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6241 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6301 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6361 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6421 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6481 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6541 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6601 gtcggatctc cctttgggcc gcctccccgc atcggtacgt atggccaggt acctttaaga
     6661 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6721 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6841 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6901 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     6961 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     7021 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     7081 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     7141 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     7201 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     7261 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     7321 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     7381 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     7441 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     7501 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     7561 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7621 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7681 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7741 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7801 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7861 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7921 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7981 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8041 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8101 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8161 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8221 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8281 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8341 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8401 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8461 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8521 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8581 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8641 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8701 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8761 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8821 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8881 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8941 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9001 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9061 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9121 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9181 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9241 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9301 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9361 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9421 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9481 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9541 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9601 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9661 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9721 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     9781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     9841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     9901 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     9961 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10021 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10081 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
    10141 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
    10201 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
    10261 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
    10321 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
    10381 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
    10441 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
    10501 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
    10561 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
    10621 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
    10681 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
    10741 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
    10801 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
    10861 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
    10921 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
    10981 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
    11041 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
    11101 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
    11161 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
    11221 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
    11281 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
    11341 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
    11401 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
    11461 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
    11521 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
    11581 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
    11641 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
    11701 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
    11761 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    11821 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    11881 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11941 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    12001 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    12061 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    12121 attgtatttg ttaaatatgt actacaaact tagtagt
//
