LOCUS       sequence-275948-        9547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo (without an ER export 
            signal) for Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pUASt-ssBiP-ESCargo*
FEATURES             Location/Qualifiers
     source          1..9547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    144..177
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    210..304
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    229..323
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        342..580
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     527..548
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     sig_peptide     606..659
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1124..1135
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     intron          1773..1838
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1968..1988
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2260..2394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2297..2316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2351..2370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2423..2492
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2711..2729)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2750..2766)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2750..2766)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2763..2785)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2774..2790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2798..2804,2805..2822,2823..2828))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2843..2864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2981..2998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3152..3740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3232..3251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3911..4702,4703..4771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4534..4553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4772..4876)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4902..5357)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5039..5060)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5251..5270
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5484..5506
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5498..5515
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5499..5515
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5522..5541
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5522..5540
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5574..5592)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6044..6115,6517..6790,6865..7519,7581..7896,8100..8231,
                     8302..8916)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt
       61 tatatatatc aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg
      121 gatttcactg gaactaggct agcataactt cgtataatgt atgctatacg aagttatgct
      181 agcggatcca agcttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc
      241 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      301 cgagcggagt actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc
      361 gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga
      421 aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag
      481 ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat
      541 ctgccaagaa gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattca
      601 ccaccatgaa gctgtgcatt ctgctcgctg tagttgcatt tgttggtttg tcgttgggcg
      661 attcgacaga aaatgtcatc aaaccattta tgcggttcaa agtacatatg gaaggtagtg
      721 ttaacggtca cgaatttgaa atcgagggcg aaggagaggg caaaccatat gaaggaacac
      781 agaccgcaaa actccaagtt actaagggtg gacctctgcc gttcgcctgg gatatactgt
      841 cgccccaatt ccagtatgga agcaaggtgt acgtaaagca cccagcggat atcccagatt
      901 ataaaaaact gtcgtttccg gaaggtttca aatgggaacg agtgatgaac ttcgaggatg
      961 gcggagttgt gactgtaaca caagacagta gtctccaaga cggcacattc atatatcatg
     1021 tcaaattcat tggagttaac tttccctcgg atggtccagt catgcagaag aaaaccctcg
     1081 gttgggagcc ttcgactgaa cgcttgtatc cacgagacgg agtattgaag ggcgaaatcc
     1141 ataaggcact gaagctcaag ggcggaggtc actatttggt agagtttaag tccatataca
     1201 tggcaaagaa gcctgtaaag ctcccaggat actactacgt ggatagtaaa ctcgatataa
     1261 cttcccataa tgaggattat actgttgtag agcagtacga gagggcagaa gcgcgtcatc
     1321 atctgtttca gggttcctcc cgaggtgtcc aggtcgaaac gataagtccg ggagatggta
     1381 ggacgttccc aaagaggggt cagaccgtgg tggtgcatta tactggaatg ttggaagacg
     1441 gtaagaaatt ggactcgtcc agggatcgca acaagccgtt taaatttatg ctgggaaaac
     1501 aggaggtcat tcgcggctgg gaagagggcg tcgcacaaat gagtgttggc cagagggcaa
     1561 aactgacaat aagtcctgac tatgcctacg gtgcgactgg ccatccgggt gtcatcccgc
     1621 cacacgcgac gctcgtcttc gatgttgagc tgctcaagct cgagtaagcg gccgcggctc
     1681 gagggtacct ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     1741 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     1801 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     1861 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     1921 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     1981 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     2041 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     2101 tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata
     2161 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2221 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2281 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2341 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccactag
     2401 tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg
     2461 gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt
     2521 ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc
     2581 tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc
     2641 ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca
     2701 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     2761 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2821 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2941 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4201 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     4921 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4981 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     5041 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     5101 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     5161 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     5221 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     5281 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     5341 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5401 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     5461 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     5521 gtaatacgac tcactatagg gcgaattggg tacgtaccgg gcccctagta tgtatgtaag
     5581 ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac
     5641 tggatatcat tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat
     5701 gtaccaggtt ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg
     5761 tttgcctcct tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc
     5821 tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt
     5881 cagcggtttc gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt
     5941 gccaaaactc ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg
     6001 gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca
     6061 ggagctatta attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag
     6121 tagtacatgt gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca
     6181 aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca
     6241 aatagtgaca cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga
     6301 gatcatatca tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa
     6361 aaaatataac aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta
     6421 taaacctact tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg
     6481 cttgtagttg atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag
     6541 agctgcatta accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt
     6601 ccgccggagg actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg
     6661 cacaatatgg acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc
     6721 aaccggacac gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg
     6781 ctcaagaacg gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg
     6841 tccaattacc aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg
     6901 tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc
     6961 cgcagggcat ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg
     7021 ccaaggagat gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc
     7081 taacggccag ggaacacctg attttccaag ccatggtgcg gatgccacga catctgacct
     7141 atcggcagcg agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc
     7201 agcacacgat catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc
     7261 gtctggcatt cgcctccgag gctctaaccg atccgccgct tctgatctgc gatgagccca
     7321 cctccggact ggactccttt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc
     7381 agaagggcaa gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct
     7441 ttgacaagat ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag
     7501 ccgtcgactt cttttcctag tgagttcgat gtgtttatta agggtatcta gtattacata
     7561 acatctcaac tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac
     7621 ttttacgtac aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc
     7681 gccaagatat gcgacaattt tgccattagc aaagtagccc gggatatgga gcagttgttg
     7741 gccaccaaaa atctggagaa gccactggag cagccggaga atgggtacac ctacaaggcc
     7801 acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa
     7861 ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa
     7921 tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagatag aattgcctga
     7981 ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaagata
     8041 aataaatttc atttaactcg cgaatattaa tgagatgcga gtaacatttt aatttgcaga
     8101 tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggtgtga
     8161 tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg
     8221 ccacgataaa tgtaagtcat gtttagaata catttgcatt tcaataattt actaactttc
     8281 taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc
     8341 cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattgccg
     8401 ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg
     8461 gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca
     8521 acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg
     8581 ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg
     8641 gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt
     8701 ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac
     8761 accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat
     8821 ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat
     8881 ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc
     8941 ttgttttttt tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag
     9001 ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt
     9061 ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac
     9121 acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt
     9181 ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt
     9241 ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc
     9301 gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga
     9361 gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc
     9421 tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac
     9481 tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa
     9541 gtgacat
//
