LOCUS       sequence-275951-        9565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo for Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pUASt-ssBiP-ESCargo
FEATURES             Location/Qualifiers
     source          1..9565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    61..94
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    127..221
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    146..240
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        259..497
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     444..465
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     sig_peptide     523..576
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1059..1070
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     intron          1708..1773
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1903..1923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2195..2329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2232..2251)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2286..2305
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2358..2427
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2646..2664)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2685..2701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2685..2701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2698..2720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2709..2725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2733..2739,2740..2757,2758..2763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2778..2799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2916..2933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3087..3675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3167..3186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3846..4637,4638..4706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4469..4488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4707..4811)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4837..5292)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4974..4995)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5186..5205
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5419..5441
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5433..5450
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5434..5450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5457..5476
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5457..5475
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5509..5527)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5979..6050,6452..6725,6800..7454,7516..7831,8035..8166,
                     8237..8851)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa ctaggctagc
       61 ataacttcgt ataatgtatg ctatacgaag ttatgctagc ggatccaagc ttgcatgcct
      121 gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      181 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
      241 cggagactct agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt
      301 caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc
      361 agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta
      421 accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat
      481 acaagaagag aactctgaat agggaattgg gaattcacca ccatgaagct gtgcattctg
      541 ctcgctgtag ttgcatttgt tggtttgtcg ttgggcgcgc ccgtaaacac cactgattcg
      601 acagaaaatg tcatcaaacc atttatgcgg ttcaaagtac atatggaagg tagtgttaac
      661 ggtcacgaat ttgaaatcga gggcgaagga gagggcaaac catatgaagg aacacagacc
      721 gcaaaactcc aagttactaa gggtggacct ctgccgttcg cctgggatat actgtcgccc
      781 caattccagt atggaagcaa ggtgtacgta aagcacccag cggatatccc agattataaa
      841 aaactgtcgt ttccggaagg tttcaaatgg gaacgagtga tgaacttcga ggatggcgga
      901 gttgtgactg taacacaaga cagtagtctc caagacggca cattcatata tcatgtcaaa
      961 ttcattggag ttaactttcc ctcggatggt ccagtcatgc agaagaaaac cctcggttgg
     1021 gagccttcga ctgaacgctt gtatccacga gacggagtat tgaagggcga aatccataag
     1081 gcactgaagc tcaagggcgg aggtcactat ttggtagagt ttaagtccat atacatggca
     1141 aagaagcctg taaagctccc aggatactac tacgtggata gtaaactcga tataacttcc
     1201 cataatgagg attatactgt tgtagagcag tacgagaggg cagaagcgcg tcatcatctg
     1261 tttcagggtt cctcccgagg tgtccaggtc gaaacgataa gtccgggaga tggtaggacg
     1321 ttcccaaaga ggggtcagac cgtggtggtg cattatactg gaatgttgga agacggtaag
     1381 aaattggact cgtccaggga tcgcaacaag ccgtttaaat ttatgctggg aaaacaggag
     1441 gtcattcgcg gctgggaaga gggcgtcgca caaatgagtg ttggccagag ggcaaaactg
     1501 acaataagtc ctgactatgc ctacggtgcg actggccatc cgggtgtcat cccgccacac
     1561 gcgacgctcg tcttcgatgt tgagctgctc aagctcgagt aagcggccgc ggctcgaggg
     1621 tacctctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     1681 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     1741 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     1801 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     1861 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     1921 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     1981 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     2041 tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag
     2101 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2161 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2221 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2281 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc actagtgtcg
     2341 acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc
     2401 ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg
     2461 tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc
     2521 gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc
     2581 agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga cactagttct agccagcttt
     2641 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     2701 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2761 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2821 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2881 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2941 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3001 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3061 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4801 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     4861 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     4921 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     4981 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5041 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     5101 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     5161 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     5221 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     5281 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5341 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     5401 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     5461 acgactcact atagggcgaa ttgggtacgt accgggcccc tagtatgtat gtaagttaat
     5521 aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac tgcactggat
     5581 atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgatgtacc
     5641 aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc
     5701 ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt
     5761 cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg
     5821 gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa
     5881 aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt
     5941 gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc
     6001 tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta
     6061 catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg
     6121 tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac aaacaaatag
     6181 tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt tttgagatca
     6241 tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt tacaaaaaat
     6301 ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac
     6361 ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca taacgcttgt
     6421 agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt cgcagagctg
     6481 cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac ccagtccgcc
     6541 ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa
     6601 tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc tggtcaaccg
     6661 gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa
     6721 gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa
     6781 ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg
     6841 ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag
     6901 ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag
     6961 gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg
     7021 gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct gacctatcgg
     7081 cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac
     7141 acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg
     7201 gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga gcccacctcc
     7261 ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag
     7321 ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga gctctttgac
     7381 aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc
     7441 gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt acataacatc
     7501 tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta
     7561 cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa
     7621 gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt tgttggccac
     7681 caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg
     7741 gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact
     7801 cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata
     7861 taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg cctgattcca
     7921 cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga agataaataa
     7981 atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt gcagatggtt
     8041 gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg tgtgatgaat
     8101 atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg
     8161 ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa ctttctaatg
     8221 aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag
     8281 tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt
     8341 tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg
     8401 agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc
     8461 cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc
     8521 gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc
     8581 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
     8641 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
     8701 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
     8761 gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc
     8821 tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt
     8881 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
     8941 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
     9001 tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg
     9061 cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata
     9121 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
     9181 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
     9241 ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt
     9301 tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa
     9361 tatatttgta tatatttccc tcacagctat atttattcta atttaatatt atgacttttt
     9421 aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac
     9481 atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg
     9541 tttatatata tcaaaaataa caact
//
