LOCUS       sequence-27596-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, 
            Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, 
            Ramalho-Santos M
  TITLE     Chd1 regulates open chromatin and pluripotency of embryonic stem 
            cells.
  JOURNAL   Nature. 2009 Aug 13;460(7257):863-8. doi: 10.1038/nature08212. Epub 
            2009 Jul 8.
  PUBMED    19587682
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08212"; journalName: "Nature"; date: "2009-08-13- 
            13"; volume: "460"; issue: "7257"; pages: "863-8"
COMMENT     Sequence Label: pSicoR-mCh-GFPi
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(176..379)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(205..225)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        380..683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggccngaggg cggccctgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
       61 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
      121 ttgctcacca tggtggcgac cggtagcgct agcggatctg acggttcact aaaccagctc
      181 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
      241 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
      301 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
      361 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
      421 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
      481 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
      541 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
      601 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
      661 catttaccgt aagttatgta acgcggaact ccatatatgg gctatganct aatgaccccg
      721 taattgatta ctatnataac taatgcatgg cggtaatacg gttatccacg cgncgcctaa
      781 tgnatctagt actcggaaaa aaagcccacg tctat
//
