LOCUS       sequence-275973-        7784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prey vector GSO1 L3_pECIA14 should be used with bait vector GSO1 
            L3_pECIA2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: GSO1 L3_pECIA14
FEATURES             Location/Qualifiers
     source          1..7784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             3080..3103
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4778..4801
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4817..4834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4857..4874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4907..5041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4931..4950)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4985..5004
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5185..5201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5185..5201)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5198..5220)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5209..5225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5233..5239,5240..5257,5258..5263))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5278..5299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5416..5433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5587..6175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5667..5686)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6346..7137,7138..7206))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6969..6988
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7207..7311)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7379..7397
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7435..7457)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7557..7576
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(7770..7784,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg caaccgggta tcatcaacaa
      541 cgatcttcag actcttctcg aggtgaagaa atctttggtc acgaatccac aagaagacga
      601 tcctctccga caatggaact ctgataacat caactactgt tcttggaccg gagtcacatg
      661 cgacaatacc ggtttatttc gtgtaattgc tctaaacctt accggtttag gattaaccgg
      721 ttcaatttca ccttggtttg gccggtttga caacctaatc caccttgatc tatcttccaa
      781 caatctagtt ggtcccatcc caactgctct ctccaacctt acttccttgg agtctctgtt
      841 tctcttttct aaccaactca ccggcgagat tccgagccag ctcggctccc tcgttaatat
      901 ccggtcgcta aggatcggag acaatgagct tgttggagat atcccggaga cgttagggaa
      961 cttggtgaat ctccagatgc ttgctctggc ttcttgtaga ctcaccggtc ctataccgag
     1021 tcagctcggt cgtctcgtcc gagttcagtc gttgatattg caagataact atctcgaagg
     1081 accgattccg gctgagttag gaaactgctc tgatctcact gtgttcactg cggcggagaa
     1141 catgctcaac gggacgatac cggcggagct tggacgcctt gaaaacctcg aaatactcaa
     1201 cctggcgaac aacagtctca ccggagagat accgagtcaa ttaggtgaaa tgagccaact
     1261 ccagtatctt agcttgatgg caaatcaact tcaaggtctt atccccaaga gtttggctga
     1321 tctgggaaat cttcaaactc ttgatttatc ggcgaataat cttaccggag agatacctga
     1381 agaattctgg aacatgagtc agcttcttga tttggttcta gcaaacaatc atctctctgg
     1441 ttcattacca aagagcattt gttctaacaa cacaaacttg gagcaactgg ttttgtctgg
     1501 aactcagctt tctggtgaaa tccctgtgga attaagcaaa tgtcaatcgc ttaagcagct
     1561 tgatttgtcg aacaattctc tcgctggctc gatccctgaa gcattgtttg agctagtcga
     1621 actcacagat ctttatctcc acaataatac cttggagggt acgttatctc cttcgatatc
     1681 aaacctcact aatctacagt ggcttgtgtt gtatcacaat aacttggaag gcaagttacc
     1741 gaaggagatc agcgcactca gaaaattaga ggttctgttt ctgtatgaga atcgtttctc
     1801 aggggaaatc ccacaggaga ttggaaactg cacaagcttg aagatgattg atatgttcgg
     1861 gaatcatttc gaaggagaga tacctccttc aataggaaga ttaaaagagc ttaacttgct
     1921 tcacttgaga cagaacgagc ttgtgggtgg acttcccgcc agtttaggta actgtcatca
     1981 actgaacatc ttggatttgg cggataatca gctctcggga tcaattcctt catccttcgg
     2041 gttcttaaag ggcttggaac agttgatgct ttacaacaat tctcttcaag gaaatcttcc
     2101 tgattcactt atcagtctga ggaacctcac cagaatcaat ctctctcaca acaggctcaa
     2161 tggtacgatt catccattat gtggttcgag ctcatatctc tcattcgatg tcaccaacaa
     2221 cgggtttgaa gatgaaatcc ccctggagct aggaaactct caaaatcttg atcgtctcag
     2281 gttaggcaag aatcaattga caggaaaaat cccttggacg ttggggaaaa tccgcgagct
     2341 gtccttgtta gatatgtcaa gcaacgctct cacaggaacc attccgctac agctcgtctt
     2401 gtgcaagaaa ctgacacaca tcgatctcaa taacaatttt ctatcaggtc ctatacctcc
     2461 atggcttgga aaactttcac agttaggaga gctcaagctt tcttcgaatc agtttgttga
     2521 gtctttacct actgaactgt tcaactgtac aaagctgctt gtgttgtctc ttgatggaaa
     2581 ttcgcttaat gggtcgattc cacaagagat tggtaacttg ggagctctca atgtccttaa
     2641 ccttgataag aaccagtttt caggttcact tcctcaagct atgggaaagc tcagcaagct
     2701 gtatgagctt cggttatcaa gaaacagctt aacaggagaa atcccagttg agattggaca
     2761 gcttcaggat cttcaaagtg ctttagacct cagctacaat aacttcactg gagatatacc
     2821 ttcgacaatt gggacattat caaagctgga aacacttgat ctttctcaca atcagcttac
     2881 aggggaagtt cctggctcgg ttggggatat gaagagtttg ggatatctca atgtttcttt
     2941 caataacctc ggagggaagc tcaagaaaca gttctcaaga tggccagctg attccttttt
     3001 aggcaacaca ggtctttgtg gaagcccact aagtcgctgc aacagagtta gaagtaataa
     3061 caagcaacaa ggtcttagtc tggaggtgct gttccaggga ccaagcggca atcccggcgg
     3121 cgatctggcc ccccagatgc tgcgcgagct gcaggagacc aatgccgccc tgcaggacgt
     3181 gcgcgagctg ctgcgccacc gcgtgaagga gatcaccttc ctgaagaaca ccgtgatgga
     3241 gtgcgacgcc tgcggcatgc agccctctag aggctccggc tccatcatcc cagttgagga
     3301 ggagaacccg gacttctgga accgcgaggc agccgaggcc ctgggtgccg ccaagaagct
     3361 gcagcctgca cagacagccg ccaagaacct catcatcttc ctgggcgatg ggatgggggt
     3421 gtctacggtg acagctgcca ggatcctaaa agggcagaag aaggacaaac tggggcctga
     3481 gatacccctg gccatggacc gcttcccata tgtggctctg tccaagacat acaatgtaga
     3541 caaacatgtg ccagacagtg gagccacagc cacggcctac ctgtgcgggg tcaagggcaa
     3601 cttccagacc attggcttga gtgcagccgc ccgctttaac cagtgcaaca cgacacgcgg
     3661 caacgaggtc atctccgtga tgaatcgggc caagaaagca gggaagtcag tgggagtggt
     3721 aaccaccaca cgagtgcagc acgcctcgcc agccggcacc tacgcccaca cggtgaaccg
     3781 caactggtac tcggacgccg acgtgcctgc ctcggcccgc caggaggggt gccaggacat
     3841 cgctacgcag ctcatctcca acatggacat tgacgtgatc ctaggtggag gccgaaagta
     3901 catgtttcgc atgggaaccc cagaccctga gtacccagat gactacagcc aaggtgggac
     3961 caggctggac gggaagaatc tggtgcagga atggctggcg aagcgccagg gtgcccggta
     4021 tgtgtggaac cgcactgagc tcatgcaggc ttccctggac ccgtctgtga cccatctcat
     4081 gggtctcttt gagcctggag acatgaaata cgagatccac cgagactcca cactggaccc
     4141 ctccctgatg gagatgacag aggctgccct gcgcctgctg agcaggaacc cccgcggctt
     4201 cttcctcttc gtggagggtg gtcgcatcga ccatggtcat catgaaagca gggcttaccg
     4261 ggcactgact gagacgatca tgttcgacga cgccattgag agggcgggcc agctcaccag
     4321 cgaggaggac acgctgagcc tcgtcactgc cgaccactcc cacgtcttct ccttcggagg
     4381 ctaccccctg cgagggagct ccatcttcgg gctggcccct ggcaaggccc gggacaggaa
     4441 ggcctacacg gtcctcctat acggaaacgg tccaggctat gtgctcaagg acggcgcccg
     4501 gccggatgtt accgagagcg agagcgggag ccccgagtat cggcagcagt cagcagtgcc
     4561 cctggacgaa gagacccacg caggcgagga cgtggcggtg ttcgcgcgcg gcccgcaggc
     4621 gcacctggtt cacggcgtgc aggagcagac cttcatagcg cacgtcatgg ccttcgccgc
     4681 ctgcctggag ccctacaccg cctgcgacct ggcgcccccc gccggcacca ccgacgccgc
     4741 gcacccgggt tctagaggaa gcacctccgg cgataacgat tacaaggatg atgatgataa
     4801 ggccagcggc accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     4861 gactgtgcct tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat
     4921 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4981 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     5041 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     5101 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt cgacctgcag
     5161 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5221 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5281 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5341 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5401 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5761 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6001 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6241 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6301 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6361 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6421 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6481 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6541 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6601 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6661 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6721 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6781 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6841 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6901 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6961 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7021 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7081 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7141 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7201 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7261 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7321 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7381 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     7441 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7501 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     7561 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     7621 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     7681 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7741 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgtt
//
