LOCUS       sequence-275977-       14209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep78 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14209)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 14209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep78
FEATURES             Location/Qualifiers
     source          1..14209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5066..5084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5152..5256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5257..5325,5326..6117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5475..5494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6288..6876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6777..6796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7065..7085)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7083..7380
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7457..8056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7457..7476)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7953..7973
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8147..8281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8419..8438)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8715..8976
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9093..9665
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9673..9692
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9720..9739
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9720..9738
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9749..10369
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9822..9841)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10411..10532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10587..10966
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10967..11170
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11121..11141
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11172..11190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11193..11211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11221..11245
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11345..11368
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11369..11386
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11936..11959
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11972..11996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(14199..14209,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     3841 ctcctgctcc tgcccctcct gcccctccts ctcctscscc tcctscycct cctgctcctg
     3901 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     3961 ctgccccctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct
     4021 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctscccctcc tcctgctcct
     4081 gcccctcctg cccctcctcc tgctcctscc cctcctccts ctcctgcycc tcctccycct
     4141 cctgcccctc ctcctsctcc tscccctcct scccctcctc cysctcctcc ycctcctcct
     4201 sctcctsccc ckcctscccc tcctscycct cctcctgctc ctgcccctcc tcctgctcct
     4261 gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     4321 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
     4381 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
     4441 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
     4501 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
     4561 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
     4621 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
     4681 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
     4741 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
     4801 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
     4861 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
     4921 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
     4981 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
     5041 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5101 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5161 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5221 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5281 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5341 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5401 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5461 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5521 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5581 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5641 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5701 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5761 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5821 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5881 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5941 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6001 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6061 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6121 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6181 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6421 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6901 cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
     6961 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     7021 gggaaggcca tccagcctcg cgtcgaacta gatgatccgg ctgtggaatg tgtgtcagtt
     7081 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7141 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7201 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7261 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7321 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7381 agggtgaccg ccacgaccgg tgccgccacc atcccctgac ccacgcccct gacccctcac
     7441 aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7501 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7561 caccgtcgac cccgaccgcc acatcgaacg cgtcaccgag ctgcaagaac tcttcctcac
     7621 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7681 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7741 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7801 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     7861 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     7921 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     7981 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     8041 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     8101 acccggtccg acgcggcccg acgggtccga ggggggtcga cctcgaaact tgtttattgc
     8161 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8221 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     8281 ctgatccgaa ccccttcctc gaccaattct catgtttgac agcttatcat cgcagatccg
     8341 ggcaacgttg ttgccattgc tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     8401 cgtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8461 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8521 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8581 tcgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa
     8641 agcggggctt cggttgtacg cggttaggag tcccctcagg atatagtagt ttcgcttttg
     8701 catagggagg gggaaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     8761 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     8821 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     8881 cgaaccactg aattccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata
     8941 aacgccattt gaccattcac cacattggtg tgcacctcca agctggccgg ggatcctcta
     9001 gggcctctga gctattccag aagtagtgaa gaggcttttt tggaggccta ggcttttgca
     9061 aaaagctccg gatcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt
     9121 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     9181 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     9241 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     9301 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     9361 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     9421 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     9481 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
     9541 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     9601 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
     9661 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt
     9721 aatacgactc actatagggc gaattgatat gtctagatta gataaaagta aagtgattaa
     9781 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     9841 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     9901 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     9961 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
    10021 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
    10081 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
    10141 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
    10201 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
    10261 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
    10321 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg
    10381 atcccgggaa ttcagatctt attaaagcag aacttgttta ttgcagctta taatggttac
    10441 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10501 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgaatcc atggcgcaga
    10561 actggtaggt atggaagatc cgcgttgaca ttgattattg actagttatt aatagtaatc
    10621 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    10681 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    10741 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    10801 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    10861 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    10921 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    10981 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    11041 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    11101 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    11161 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
    11221 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
    11281 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttaccac
    11341 catggattat aaagacgatg atgacaagca ccaccaccac caccacgaca aggattgtga
    11401 aatgaaacgc accacactgg acagcccttt ggggaagctg gagctgtctg gttgtgagca
    11461 gggtctgcac gaaataattt ttctgggcaa ggggacgtct gcagctgatg ccgtggaggt
    11521 cccagccccc gctgcggttc tcggaggtcc ggagcccctg atgcaggcta cagcctggct
    11581 gaatgcctat ttccaccagc ccgaggctat cgaagagttc cccgtgccgg ctcttcacca
    11641 tcccgttttc cagcaagagt cgttcaccag acaggtgtta tggaagctgc tgaaggttgt
    11701 gaaattcgga gaagtgattt cttactctca cttagcagcc ctggcaggca accccgcagc
    11761 cacggcagca gtgaagacgg cactgagtgg caatcctgtc cctatcctga tcccgtgcca
    11821 cagagtggtc cagggggatt tggatgtggg cggttacgag ggtggactgg ccgtgaagga
    11881 atggcttctg gcccatgaag gccaccggtt ggggaagcca ggcttgggat tcgaactgga
    11941 ggtgctgttc cagggccccg gtaccgccat cacaagtttg tacaaaaaag caggcttgaa
    12001 ggaattcggt accatgatcg actccgtgaa gctgcgccgc gacagcgcgg cggacttctt
    12061 ctcccactac gagtacctgt gcgcgctgca gaactccgtg ccgctgcccg ccgtgcgcgc
    12121 ctgtctccgg gagggcgtgc tggatttcaa cgccgacggc ctccgcgggg tggactgggc
    12181 gcctctgctg agcaccctca agatcaataa agacctgccc ttggtctcca tcaagagctt
    12241 cttccagccc tggctggggg acacaggttc tgacatgaat aaattttgca gaagtcgtgt
    12301 tcctgcgata agatacaaag atgtgacctt ccagttgtgt aaagctctta aaggctgttt
    12361 aagtatatca agtgtgctaa agaacctgga gctaaatgga ctaattctga gagagaggga
    12421 tttaactatt ctagcaaagg gattgaataa atcggcttct ttggtgcacc tgtctcttgc
    12481 aaattgtcca attggagatg gaggtttaga aattatttgt caaggtataa ggagctctat
    12541 cactcttaag acagtcaact tcacaggatg taatctgaca tggcagggag cagatcacat
    12601 ggccaagatc ttaaagtatc agaccatgag aaggcatgaa gaaacctggg ctgagagtct
    12661 tcgctatagg agacctgatc ttgactgtat ggctggctta agacgtatca cactgaattg
    12721 caacacactt attggtgacc taggtgcatg tgcttttgca gactctctca gtgaggattt
    12781 atggctgaga gctcttgacc tgcaacagtg cggcctcacc aatgaaggag caaaggcttt
    12841 gctagaggcc cttgaaacca atacaactct ggtcgttctg gatataagaa aaaatccact
    12901 cattgatcat tctatgatga aagcagttat caaaaaagtc ctccagaatg gaaggagtgc
    12961 caaatcagag taccagtgga taacttctcc atcagtgaag gaaccatcca aaactgctaa
    13021 acagaaaagg agaactataa ttctaggaag tggtcacaaa ggaaaagcta ctattagaat
    13081 tgtaggattg gctacaaaga aacctgtaag tagtggcaga aaacactccc ttggtaaaga
    13141 atattatgcg cccgcacctc ttccacctgg tgtgtctggt ttcttgccgt ggcgtactgc
    13201 agaacgtgca aaaagacaca ggggtttccc attaatcaaa acacgtgata tatgtaatca
    13261 gttgcagcaa ccaggttttc ctgtgactgt gacagtagag agtccttcat cctctgaagt
    13321 tgaagaggtt gatgattctt cagagagtgt tcatgaagtg cctgagaaaa ctagtataga
    13381 acaagaagca ttacaggaaa aactggagga gtgcctaaag cagttaaagg aagaaagagt
    13441 gataaggctt aaggttgata aacgagtcag tgagctggaa catgaaaatg cccagttaag
    13501 aaatataaat ttctctttgt ctgaagccct tcatgcacag tcattgacaa atatgatcct
    13561 ggatgatgaa ggtgttttgg gcagcattga gaattctttt cagaagtttc atgctttctt
    13621 ggatctcctt aaagatgctg ggcttgggca gcttgccaca atggctggga tagatcagtc
    13681 agattttcaa ttactaggtc atccccagat gacttctact gttagtaatc cacctaaaga
    13741 agaaaagaag gcgcttgaag atgaaaaacc agaaccgaag cagaatgccc tagggcaaat
    13801 gcaaaatatc caggtttcta tttgtatgca gtcagcttac aatgaaggaa cactaatgaa
    13861 gtttcagaaa attacaggtg atgctagaat tcctttgcct ctcgactcct ttcctgtccc
    13921 agtttctact ccagagggct taggaacttc cagcaacaac ctaggagtcc cagctactga
    13981 gcagcggcag gagtcttttg aaggattcat tgctagaatg tgttctcctt caccagatgc
    14041 gacttctgga actggaagtc aaagaaaaga agaggagttg tccagaaata gcagatcttc
    14101 ttcagagaaa aagaccaaaa caggtgaata taccaaaaaa cactctgata agcaacaccc
    14161 tggaaaggac ctgcattcct agctcgagtg cggccgcaac ccagctttc
//
