LOCUS       sequence-275979-       19079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19079)
  AUTHORS   Santos TM, Han S, Bowser M, Sazani K, Beauchamp RL, Murthy V, Bhide 
            PG, Ramesh V
  TITLE     Alternative splicing in protein associated with Myc (Pam) influences
            its binding to c-Myc.
  JOURNAL   J Neurosci Res. 2006 Feb 1;83(2):222-32.
  PUBMED    16342205
REFERENCE   2  (bases 1 to 19079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Res."; date: "2006-02-1"; volume: "83(2)"; pages: "222-32"
COMMENT     Sequence Label: pCMV-Pam-FL
FEATURES             Location/Qualifiers
     source          1..19079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1317..1335
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      1367..1376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1376..1405
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(16205..16221)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16206..16222)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(16277..16296)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(16278..16296)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    16570..16691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(16607..16626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     16661..16680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(16698..17153)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16835..16856)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     17047..17066
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        17180..17284
                     /gene="bla"
                     /label=AmpR promoter
     promoter        17286..17643
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        17286..17477
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(17309..17329)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      17494..17629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             17678..18472
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             17678..18472
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(17732..17751)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     18342..18361
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(18660..18679)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    18704..18751
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg attacgccaa gctcgaaatt
     1321 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ccatggagca
     1381 gaaactcatc tctgaagagg atctgagccc gggcggatcc cccgggctgc aggaattcga
     1441 tcgcggatcc gtcgacatgc cggttcccga cggctccgtg gctgctgcgg ggctggggct
     1501 ggggctaccc gccgcggact cccggggtca ctaccagctg ctgctgtcag gccgggccct
     1561 ggccgaccgc taccggagga tttataccgc tgcgctcaat gacagggacc aggggggcgg
     1621 cagcgctgga cacccagcct ccaggaataa gaaaatttta aataagaaga aattgaaaag
     1681 aaaacagaag agcaaatcaa aagtgaagac aagaagcaag tctgaaaact tagagaatac
     1741 agtaatcata ccagatatca aactacatag caatccttct gctttcaata tttactgtaa
     1801 tgtacgccat tgcgttctgg aatggcagaa gaaggaaata tcattggcag ccgcatctaa
     1861 gaactctgtg cagagtggag aatcagatag tgatgaagaa gaggaatcca aagagccccc
     1921 tatcaagctt ccaaagatta ttgaggttgg cctttgtgaa gtttttgaat tgatcaaaga
     1981 gacacgattt tctcatccat ccctgtgtct caggagtctc caagccctgc tcaacgtgct
     2041 gcagggccag cagccagaag gcctccagtc tgagccacct gaggtcctag agtctctctt
     2101 ccagcttctt ttggaaatca ccgttcgaag tactgggatg aatgacagca caggacagtc
     2161 cttaacagca ctttcctgtg cttgcctctt tagtctggtg gcttcttggg gagaaacagg
     2221 aaggacactt caggccatct ctgctatcct caccaacaat ggaagccatg cttgccaaac
     2281 tattcaggtg ccaacaattc taaattcgct acagagaagt gtacaagcag ttttggtggg
     2341 aaaaattcaa attcaggact ggtttagtaa tggcattaag aaagcagctt taatgcacaa
     2401 gtggccatta aaagaaatat ctattgatga agatgaccaa tgtctacttc agaatgatgg
     2461 attttttctt tatctattat gcaaggatgg attatataaa ataggctctg gatacagtgg
     2521 aacagttagg ggccatatat acaattctac atcccgtatt agaaacagaa aagaaaaaaa
     2581 gtcttggtta gggtatgctc agggttattt attatataga gatgtgaata accacagcat
     2641 gacagccata aggataagcc ctgaaacact ggagcaagat ggtactgtga tgttaccaga
     2701 ttgccacact gaaggtcaaa atattttatt cactgatgga gaatatatta atcagatagc
     2761 tgcttcaaga gatgatggct ttgttgtcag aatatttgcc acaagcactg aacctgttct
     2821 acagcaagaa ttgcaactta aactggctag aaaatgctta catgcctgtg gtatctcact
     2881 attcgatctg gaaaaggact tgcatattat aagtacagga tttgatgagg agtcagcaat
     2941 tcttggtgca ggacgagagt ttgcgctaat gaaaacagca aatggaaaga tatattacac
     3001 tggcaaatac cagagtcttg gaatcaaaca aggtggtcct tcagcaggaa aatgggttga
     3061 gctaccaatt acaaaatctc caaagatagt acacttctca gttggacacg atggctctca
     3121 cgccctttta gttgcagaag atgggagcat attctttaca ggatctgcta gtaaaggaga
     3181 agatggagaa tcaactaaga gcagacggca atccaaacct tataaaccta aaaagataat
     3241 taagatggaa ggaaagattg tggtatatac agcctgcaat aatggaagta gttctgttat
     3301 ttctaaagat ggagaactct acatgtttgg aaaagatgcc atttactctg atagttcaag
     3361 tttggtaact gatttgaagg gccattttgt aactcaggta gctatgggca aagctcacac
     3421 ttgtgtttta atgaagaatg gagaggtgtg gacatttggt gtaaataata aaggacagtg
     3481 tggacgagat actggtgcca tgaaccaagg tgggaaaggg tttggagttg aaaatatggc
     3541 aacagcaatg gatgaagacc tggaagaaga actagatgaa aaagatgaga agtctatgat
     3601 atgccctcca ggcatgcaca aatggaagct ggagcagtgc atggtttgca ctgtctgtgg
     3661 agactgtaca ggttatggag ccagctgtgt cagtagtgga cggccagaca gagtccccgg
     3721 agggatctgt ggttgtggtt ccggagaatc tggttgtgct gtgtgtggat gttgcaaggc
     3781 ctgtgcaaga gagttagatg gtcaagaggc aagacaaaga ggaattcttg atgcagtgaa
     3841 agaaatgata cctttagatc ttcttttagc tgtcccagtg cccggggtta acattgaaga
     3901 acaccttcag ttacgacaag aagaaaaacg gcaacgtgta atcagaaggc acagattaga
     3961 ggaaggaaga gatggaagtg gagaaagagg cgaaaaggat gcaagcaaaa tcacaacata
     4021 ccctccaggc tctgtgcgat ttgactgtga gctccgggca gtccaagtca gctgtggatt
     4081 tcaccattca gtggttttaa tggaaaatgg agatgtctat acatttggtt atgggcagca
     4141 tgggcagcta ggacatggag atgtcaactc caggggatgt cccactcttg ttcaagcatt
     4201 gccaggccct agcacacaag tcactgcagg cagcaaccat acggcagtac ttttaatgga
     4261 tggacaggtc ttcacatttg gaagtttttc taaaggacaa ctgggcagac caattttgga
     4321 tgtgccatat tggaatgcaa agccagctcc catgcctaac attggatcaa aatatggaag
     4381 aaaagctact tggataggtg caagtgggga ccaaactttt ttacgaattg atgaagcact
     4441 tattaattct catgtacttg ctacatcaga aatttttgcc agtaaacaca taataggctt
     4501 ggtacctgct tctatatcag aacctcctcc atttaaatgc cttctgataa ataaagtgga
     4561 tgggagttgt aaaactttta atgactcaga acaagaggat ctgcaaggat ttggtgtgtg
     4621 tcttgatcct gtatacgatg taatttggag gtttcgacca aatactagag agctgtggtg
     4681 ttacaatgcg gtggttgctg atgccaggct tccctctgca gcagacatgc agtccagatg
     4741 tagtatccta agtcctgaac ttgccttacc aacaggatca agggccctca ctacccgatc
     4801 tcatgcagct ttgcacattt taggttgtct tgataccttg gcagctatgc aggacttaaa
     4861 aatgggtgtt gcaagtacag aggaagagac tcaagcagta atgaaggttt attctaaaga
     4921 agattatagt gtggtaaaca ggtttgaaag tcatggagga ggctggggtt attctgccca
     4981 ttcagtagaa gctatacgtt tcagtgccga cactgatatt ttacttggtg gtcttggtct
     5041 gtttggaggt agaggagaat atactgctaa aattaagctg tttgaattgg gtcctgatgg
     5101 aggagatcat gaaactgatg gtgaccttct tgcagagact gatgtattgg cttatgactg
     5161 tgctgctaga gaaaaatatg caatggtgtt tgatgagcct gttctcctgc aagctgggtg
     5221 gtggtatgtg gcatgggccc gagtgtcagg acccagcagt gactgtggat ctcatggaca
     5281 ggcatctatt accacagatg atggggttgt tttccagttc aagagttcaa agaaatcaaa
     5341 taatggtaca gatgttaatg cgggtcagat acctcagtta ttatacagac ttccaaccag
     5401 tgatggcagt gcttcaaaag gcaaacagca aaccagtgaa cctgtacaca ttttaaagag
     5461 gtcttttgca agaactgtct cagtggaatg ttttgagtca ttgttgagta ttcttcactg
     5521 gagctggacc accttagtct taggagttga agaacttaga ggattaaaag gattccagtt
     5581 cacagctaca ctcctagatt tagagagact gcgctttgtg ggtacctgtt gtctgaggtt
     5641 attgcgtgtc tatacctgtg aaatttaccc agtgtcagct acaggaaaag cagttgtaga
     5701 agaaactagc aaattagcag agtgtattgg aaaaaccaga actttgttaa gaaaaatttt
     5761 atcagaagga gttgatcact gcatggtgaa attggataat gatcctcaag gatatctcag
     5821 tcaacccttg agtcttctag aagctgtcct tcaggaatgt cataatactt tcactgcctg
     5881 ctttcattct ttctacccaa ctcctgcctt acagtgggct tgcctttgtg atctgctgaa
     5941 ttgtttggat caggatatcc aagaagcaaa cttcaagaca tcaagtagcc gactccttgc
     6001 agctgttatg tcagctctgt gtcacacgtc tgttaagctg acttccatct tcccgattgc
     6061 gtatgatgga gaagtattac tacgatcaat tgttaaacaa gttagtacag agaacgactc
     6121 aacactagtt catcgttttc cccttttggt ggcacatatg gaaaaactca gccagagtga
     6181 agagaatatc tcagggatga caagcttccg tgaagttctg gagaaaatgc tggtcattgt
     6241 tgtgctacca gtcaggaaca gcctgaggag agaaaatgaa ctcttctcct cccacctcgt
     6301 ctctaacacc tgtggattac tggccagcat tgtcagtgaa ctgacagtgt cagccctggg
     6361 atctgaggtt gatggactta attctcttca ctctgtaaaa gctagtgcta accgatttac
     6421 aaaaacaagt cagggcagaa gttggaacac tgggaacggg tcccctgatg caatctgttt
     6481 ttcagtagac aaacctggaa tagttgtggt tggtttctct gtctatggag gaggtggaat
     6541 tcatgaatat gaattagagg tgttagttga tgatagtgaa catgcaggag attcaactca
     6601 ttcccacaga tggacatctc tggaattagt gaaaggaacg tacacaacgg atgactcacc
     6661 cagtgatata gctgagatca gacttgacaa agtggttcct ttaaaggaaa atgttaaata
     6721 tgctgtgcgc ttgaggaact atggaagccg tacagccaat ggagatggag gaatgaccac
     6781 agttcagtgc cctgatggtg tgacattcac attcagcacg tgcagcttga gcagtaacgg
     6841 cacaaaccaa accagaggac agatcccaca gatactctac tataggagtg aatttgatgg
     6901 agatttacaa tcccaacttc tgagtaaagc caatgaagaa gataaaaact gtagcagagc
     6961 attgtctgtt gtaagcactg tcgttcgagc ctctaaggac ctcctgcaca gagctcttgc
     7021 tgtggatgct gatgacattc cagaactgct gagttcttcc agtctgtttt ccatgctgct
     7081 cccccttatt atagcctaca taggaccagt agctgctgct attcccaagg tggctgtaga
     7141 agtctttggc cttgtccaac aattgcttcc gtcagttgcc attttgaatc agaagtatgc
     7201 accgcctgcc ttcaacccta atcagtcgac agatagcacc acaggaaacc agcctgaaca
     7261 gggcctctct gcttgtacaa cctccagtca ctatgctgtc atagagagtg agcacccgta
     7321 taaacctgcc tgtgtgatgc attacaaggt gacattccca gaatgcgtga ggtggatgac
     7381 aatcgaattt gaccctcagt gtggtactgc acagtcagaa gatgtccttc gtttgttgat
     7441 tcctgtcaga actgttcaga attcaggata tggaccaaaa ttgacatctg ttcatgaaaa
     7501 tcttaattca tggatagaat taaagaaatt ttcaggatcc tctgggtggc ctactatggt
     7561 tttggtgttg ccaggaaatg aggccctttt ttcattggag actgcatcag attatgtgaa
     7621 agatgacaaa gcttctttct atggttttaa gtgttttgca attggatatg aatttagccc
     7681 tggacctgat gagggagtca tccaattgga aaaagaatta gccaatcttg gtggggtttg
     7741 tgcagcagct ctgatgaaga aggacctagc acttcctatt ggtaatgaat tagaagaaga
     7801 ccttgaaatt cttgaggagg ctgcattgca ggtgtgcaaa acccattctg gaattcttgg
     7861 aaagggtcta gctctttctc attcaccaac tatattagaa gcacttgagg gaaatttacc
     7921 actccaaatc caaagcaatg aacagtcttt tctggatgat tttattgcct gtgtcccagg
     7981 atcaagtggt ggaaggcttg caaggtggct tcagccagat tcatatgcgg atcctcagaa
     8041 aacatctttg atcctgaata aggatgatat tcgttgtggt tggcctacca ccataactgt
     8101 tcaaacaaaa gaccagtatg gggatgtggt acatgttccc aatatgaagg cggaagtgaa
     8161 agctgtccct gtttctcaga aaaaaatgtc tttacaacaa gatcaagcaa agaaacctca
     8221 aaggattcct ggcagtcctg cagtaacagc tgcatcttct aatactgaca tgacttatgg
     8281 agggctggca tcaccaaagc tagatgtttc atatgaacca atgatagtga aggaagctcg
     8341 atatattgcc ataacaatga tgaaggttta tgaaaattat tcatttgaag aactacgttt
     8401 tgcatcacca actcctaaga gacccagtga gaatatgctg atccgtgtca ataatgatgg
     8461 gacttattgt gcaaattgga ctccaggggc tattggactc tacactcttc atgttaccat
     8521 tgatggcatt gaaatcgatg ctggtctgga agtaagagta aaagacccac caaaagggat
     8581 gataccacca ggaactcagt tggtcaaacc aaagtctgaa cctcagccta ataaggttcg
     8641 aaaatttgtg gccaaggaca gtgcggggct tcgcatccgt agccaccctt cccttcagag
     8701 tgagcagata ggcatagtga aagtcaatgg aactatcact tttattgatg agatccataa
     8761 tgatgatggt gtgtggctga ggctgaatga tgagacaata aagaagtatg tccctaacat
     8821 gaatggttac actgaagcct ggtgcctctc ttttaatcaa catcttggca agagtcttct
     8881 ggtccctgtt gacgaatcta aaactaatac tgatgacttt ttcaaagaca taaactcctg
     8941 ctgcccacag gaagcaacaa tgcaagaaca agatatgcca ttcttgcgag gagggccagg
     9001 catgtacaag gtagtgaaga cgggaccttc aggtcacaac atcagaagct gccctaacct
     9061 tagaggtatc ccaattggaa tgttagttct gggaaacaaa gtcaaagcag tgggagaggt
     9121 aaccaattct gaagggacat gggtgcaact ggatcagaac agcatggtag agttctgtga
     9181 gagtgatgaa ggagaggcat ggtccttagc tagagacaga ggcggaaacc agtacctccg
     9241 acatgaagat gaacaagctc ttctggatca gaattctcaa actcctcctc caagcccttt
     9301 ctcagtgcaa gcttttaata aaggggcaag ttgcagtgcc caaggatttg attatggact
     9361 cggaaatagc aaagttttga ctgttttacc cactgcaggt gaccaactga gtgccatatt
     9421 gaattccatt cagtcacgac ccaatctccc agctccttcc atctttgatc aagctgcaaa
     9481 acctccctct tccctagtac acagcccatt tgtgttcgga cagccccttt ccttccagca
     9541 gcctcagctt cagagtgatc gaggaaacat ctcaacatct tctaaaccag cctctacatc
     9601 aggaaaatca gagctgtcct ctaaacacag cagatcgctt aaacctgatg gacgtatgag
     9661 ccggactact gctgatcaga agaagccaag gggcacagaa agtttatctg ctagtgaatc
     9721 cctcatctta aaatctgatg ctgcaaagtt gaggtcagat tcccacagta ggtcattatc
     9781 ccccaaccat aacaccttgc agacattgaa atctgatggg aggatgcctt ctagctccag
     9841 agctgaatcc ccaggaccag gttctcggtt gtcgtctcct aagccaaaga ctctcccagc
     9901 caataggtct agcccatcgg gtgctagttc tccacgctcc tcctcaccac atgataaaaa
     9961 tctacctcaa aaaagtactg ctcctgttaa gacaaagctt gatcctcctc gggaacgttc
    10021 taaatcagac tcttacacac ttgatccaga taccctccgc aagaagaaaa tgcccctcac
    10081 agaacctttg agaggacggt caacgtcacc aaaaccaaaa tcagtaccaa aggattctac
    10141 agattcccct ggatctgaaa atagagctcc ctctccccat gtggtacagg aaaacctcca
    10201 cagtgaggtg gtcgaagtct gcacctcaag tactttaaaa acaaatagtc taacagacag
    10261 cacctgcgat gacagcagtg aatttaagag tgtggatgaa ggttcaaata aagttcattt
    10321 tagcattgga aaagcaccac tgaaagatga acaggaaatg agagcatctc ccaaaataag
    10381 tcgaaaatgt gctaatagac acaccaggcc caaaaaagaa aaatcgagtt ttcttttcaa
    10441 aggagatgga tccaagcctt tagagccagc caagcaagcc atgtctcctt ctgtggccga
    10501 atgtgccaga gctgtgtttg cttccttcct ctggcatgaa ggcatagtac atgatgcaat
    10561 ggcttgttct tctttcctaa agtttcatcc tgaactttcc aaagaacatg ctcctataag
    10621 gagtagttta aatagccaac aacctacaga ggaaaaagaa accaagttaa aaaatagaca
    10681 ttcattagaa atatcatctg cactgaatat gtttaatatt gcaccccatg gaccagatat
    10741 atctaagatg ggtagcatca acaaaaacaa ggtattgtct atgcttaagg aaccacctct
    10801 gcatgaaaaa tgtgaggatg ggaaaaccga gaccactttt gaaatgtcca tgcataacac
    10861 aatgaagtct aagtctcctc ttcccttaac tttacaacat ttagtggctt tttgggaaga
    10921 catctctttg gctactatca aagctgcttc ccagaatatg atttttccaa gtcctggttc
    10981 ctgtgcagtt cttaaaaaga aagagtgtga gaaagagaat aagaagtcca aaaaggaaaa
    11041 aaagaaaaaa gaaaaggcag aagttaggcc caggggtaat ttgtttggag agatggccca
    11101 gctggcagta ggaggaccag agaaagatac catctgtgaa ctgtgtgggg agtcacatcc
    11161 atacccggtg acctatcaca tgagacaagc tcacccaggt tgtggccgat atgctggtgg
    11221 acaaggttac aatagcattg ggtatttttg tggaggatgg gctggtaact gtggtgatgg
    11281 tggcatagga ggaagcactt ggtatctggt atgtgatcgc tgtagagaaa aatacctccg
    11341 cgaaaaacag gctgctgcaa gggagaaggt caaacaatct aggagaaagc caatgcaagt
    11401 caagacccct cgtgccttgc ccaccatgga agctcaccag gtgattaaag ccaatgcact
    11461 cttcctgctg tccctgagca gtgcagcaga accgagcatt ctgtgttacc atcctgcaaa
    11521 gccattccaa tctcagttgc ccagtgtaaa agaaggcatt tctgaggatc ttcctgtgaa
    11581 aatgccttgt ctctacctgc agacattagc taggcatcat catgaaaatt ttgtgggcta
    11641 tcaagatgac aatctattcc aggatgaaat gagatatcta cgttcaacat ctgtacctgc
    11701 cccgtatata tcagtaactc ctgatgcaag tcctaatgta tttgaagagc cagagagcaa
    11761 tatgaagtct atgccaccaa gtttagaaac cagtcccata actgatactg atcttgcaaa
    11821 gagaactgtc ttccaaagat catactcagt tgttgcttcc gaatatgata aacaacactc
    11881 cattttacct gcacgagtta aagctattcc tagaagaaga gttaacagtg gagacactga
    11941 agttggttct tcccttttga gacatccgtc tcctgagctt tctcggctaa tctcagccca
    12001 cagctctctt tctaaaggag aacgaaattt ccagtggcca gttttagctt ttgttataca
    12061 acatcatgat ctagaaggtc ttgaaatagc aatgaaacag gccctaagga aatctgcttg
    12121 tcgagttttt gctatggagg ctttcaactg gcttctgtgt aatgtcatcc aaaccacttc
    12181 tctccatgat attctgtggc attttgtggc atcactgact cctgcaccag tggaaccaga
    12241 ggaagaagag gatgaagaaa ataaaacaag caaagaaaat tcagaacaag agaaagatac
    12301 aagagtatgt gaacatccac tctcagacat agtgattgcc ggggaagctg ctcatccttt
    12361 accacacacc tttcaccgct tgctgcagac catctcagac cttatgatgt ctctccccag
    12421 cggcagttca ttacagcaaa tggccctgag gtgctggagt ctcaaattca agcaatctga
    12481 tcaccagttc cttcatcaga gcaacgtctt tcatcacatt aacaatattt tgtcaaagtc
    12541 agatgatggc gatagtgaag agagttttag catcagtata cagtctggct ttgaagctat
    12601 gagtcaggaa ttatgcatag taatgtgctt aaaggactta accagcattg ttgacataaa
    12661 aacttcaagc cgacctgcca tgattggcag tttgacagac ggctccacag aaaccttttg
    12721 ggaatcagga gatgaagata aaaacaaaac taagaacatc accatcaact gtgtaaaagg
    12781 aatcaatgcc cgctatgtgt ctgttcacgt ggacaattcc cgagatcttg ggaataaagt
    12841 tacctcaatg accttcttaa ctggcaaagc agtagaagat ttgtgcagaa taaagcaggt
    12901 tgatctggat tccaggcaca ttggctgggt aacaagtgaa cttccaggag gggataatca
    12961 catcataaaa attgaattaa aaggcccaga aaatacactg agagttcgac aagtcaaagt
    13021 cctgggctgg aaagatggtg aaagcacaaa aatagctggc cagatttcag ccagtgtggc
    13081 ccagcagagg aactgtgaag ctgagactct gcgagtattc agactgatta cgtctcaagt
    13141 atttggaaag ctcatctctg gagatgctga acctacacca gaacaagagg aaaaagcact
    13201 attgtcatca cctgaaggag aagaaaaagt atacaatgca acatcagatg ctgacctgaa
    13261 agaacatatg gttggaatca tattcagcag gagtaagctg actaacttac aaaaacaggt
    13321 gtgtgctcat attgtccaag ctattcgcat ggaagctacc agagtccgtg aagaatggga
    13381 acatgctata tcaagcaaag aaaatgccaa ttctcagcca aatgatgaag atgcctcctc
    13441 tgatgcctac tgctttgagc tgctctctat ggttttagca ctgagtggct ctaacgttgg
    13501 ccggcaatat ctggctcaac agctaaccct gcttcaggat ctcttctcgc tgcttcacac
    13561 agcctctcct agagtccaga gacaggtaac ctctttacta agaagagttt tgcctgaagt
    13621 aacccctagt cgtctggcca gcatcatagg agtgaaatcc ctccccccag cagatatcag
    13681 tgatatcatt cactcaacag agaaaggaga ctggaataag ctgggtatct tggacatgtt
    13741 tctaggatgc attgccaaag cactcactgt acagctaaaa gccaaaggaa ccaccatcac
    13801 tggaacagct ggtaccactg tgggcaaagg agttacaaca gttactcttc cgatgatttt
    13861 caattccagt tatctccgac gaggtgaaag tcattggtgg atgaagggct caacccctac
    13921 ccagatctca gagatcatca ttaaacttat caaggatatg gcagcaggtc atctgtcaga
    13981 agcttggtcc cgagtgacaa aaaatgctat tgcagaaacc atcattgcct tgaccaagat
    14041 ggaagaagaa tttaggtctc cagtgagatg tattgcaaca actagactct ggcttgctct
    14101 cgcatcccta tgtgttcttg atcaggacca cgtagatcgt ctctcctcgg ggagatggat
    14161 gggaaaggat ggacaacaaa aacaaatgcc tatgtgtgat aaccatgatg atggtgaaac
    14221 tgcagcaatc attttatgca atgtctgtgg aaatttatgt acagactgtg acagattcct
    14281 tcaccttcat cgaagaacca aaactcatca aagacaggtc ttcaaagaag aagaagaagc
    14341 tataaaggtt gaccttcatg aaggttgtgg tagaaccaaa ttgttctggt tgatggcact
    14401 ggcagattct aaaacaatga aggcaatggt ggaattccga gaacacacag gcaaacccac
    14461 cacgagtagc tcagaagcat gtcgcttctg tggttccagg agtggaacag agttatctgc
    14521 tgttggcagt gtttgttctg atgcagattg ccaggaatac gctaagatag cctgtagtaa
    14581 gacgcatcct tgtggccatc catgcggggg tgttaaaaac gaagagcact gtctgccctg
    14641 tctacacggc tgtgacaaaa gtgccacaag cctgaagcaa gacgccgatg acatgtgcat
    14701 gatatgtttc accgaagcgc tctcggcagc accagccatt cagctggatt gtagtcacat
    14761 attccactta cagtgctgtc ggcgagtatt agaaaatcga tggcttggcc caaggataac
    14821 atttggattt atatcttgtc ccatttgcaa gaacaaaatt aatcacatag tactaaaaga
    14881 cctacttgat ccaataaaag aactctatga ggatgtcaga agaaaagcct taatgagatt
    14941 ggaatatgaa ggtctgcata agagtgaagc tatcacaact cctggtgtga ggttttataa
    15001 tgacccagct ggctatgcaa tgaatagata tgcatattat gtgtgctaca aatgcagaaa
    15061 ggcatatttt ggtggtgaag ctcgctgcga tgctgaggct ggacggggag atgattatga
    15121 tcccagagag ctcatttgtg gtgcctgttc tgatgtttcc agggctcaga tgtgtcccaa
    15181 acatggcaca gactttttgg aatataaatg tcgctactgc tgttcagtgg ctgttttttt
    15241 ctgttttgga acaacacatt tttgtaatgc ttgtcatgat gattttcaaa gaatgactag
    15301 cattcctaag gaagaactac cacactgtcc tgcaggtccc aaaggcaagc agttagaagg
    15361 aactgaatgt ccactccatg ttgttcatcc acccactggg gaagagtttg ctctgggatg
    15421 tggagtgtgc agaaatgccc acacttttta gaacacgcag atcctttgtc tacagagaga
    15481 aaaattgcct tcatccccca agaggatgcg gtgaagttta aactctgctc aggataagga
    15541 cgggaccatt tttacatcca tgaaaatgaa ccattcacag tgcaagaagg ataccaaata
    15601 ccatgtacat aattcttgct atgaaaagtt tccccattat tttggtttat cttcttttga
    15661 acaaatgaca tcaaacttgt gaggtgtttg catgtggcca ttaccgtcat tggcctgtga
    15721 agcattggac atttatagat aattgatata aaagaatcgc catgcccatg gactaagaac
    15781 gatgctggct ttcaagcaaa aaagaaaaat aatcattgtt tattgtatac tgcctttttg
    15841 taatcctgta caattgcatc acgggtgggg ataaaaagag gaatattctg gtttatttcc
    15901 tagactgtta tttaaaaaaa aaaaaacatt gtgttaggac agcatataaa tgtaataagt
    15961 atcacactgt atataaacat atcaatgttt gtcctgtata agaattacta aattacaaat
    16021 gcaatttcat ttaaacttct aggttaagtt tgagcctgaa attttaatga agtgcaatac
    16081 tgagtgtgcc tcattatctt gcagctgtaa acatattgga atgtacatgt caataaaacc
    16141 actgtacatt tttatacagt gataaagtct aaaaaaaaaa aaaaagggcg gccatcaagc
    16201 ttatcgatac cgtcgacctc gagggggggc ccggtacctt aattaattaa ggtaccaggt
    16261 aagtgtaccc aattcgccct atagtgagtc gtattacaat tcactcgatc gcccttccca
    16321 acagttgcgc agcctgaatg gcgaatggag atccaatttt taagtgtata atgtgttaaa
    16381 ctactgattc taattgtttg tgtattttag attcacagtc ccaaggctca tttcaggccc
    16441 ctcagtcctc acagtctgtt catgatcata atcagccata ccacatttgt agaggtttta
    16501 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    16561 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    16621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    16681 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    16741 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    16801 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    16861 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    16921 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    16981 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    17041 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    17101 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
    17161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    17221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    17281 gaatcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
    17341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
    17401 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    17461 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    17521 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    17581 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    17641 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
    17701 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
    17761 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
    17821 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
    17881 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
    17941 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
    18001 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
    18061 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
    18121 ggaagccggt cttgtcgatc aggatgatct ggacgaagaa catcaggggc tcgcgccagc
    18181 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
    18241 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
    18301 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
    18361 tgctgaagaa cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
    18421 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
    18481 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
    18541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    18601 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
    18661 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
    18721 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    18781 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    18841 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    18901 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
    18961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    19021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
