LOCUS       sequence-27599-e         513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes both 
            mCherry and shRNA to be recombined out of the construct, turning OFF
            shRNA expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, 
            Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, 
            Ramalho-Santos M
  TITLE     Chd1 regulates open chromatin and pluripotency of embryonic stem 
            cells.
  JOURNAL   Nature. 2009 Aug 13;460(7257):863-8. doi: 10.1038/nature08212. Epub 
            2009 Jul 8.
  PUBMED    19587682
REFERENCE   2  (bases 1 to 513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08212"; journalName: "Nature"; date: "2009-08-13- 
            13"; volume: "460"; issue: "7257"; pages: "863-8"
COMMENT     Sequence Label: pSicoR-mCh-empty
FEATURES             Location/Qualifiers
     source          1..513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..140)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg
       61 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa cttcgtatag
      121 catacattat acgaagttat acatgtcaca aaaggaaact caccctaact gtaaagtaat
      181 tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgttaacgc gcggtgaccc
      241 tcgagtacta ggatccatta ggcggccgcg tggataaccg tattaccgcc atgcattagt
      301 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      361 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      421 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      481 gtggagtatt tacggtaaac tgcccacttg gca
//
