LOCUS       sequence-275999-       15713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase 
            subunits: RpoA, RpoB, RpoC(His)10, RpoZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15713)
  AUTHORS   Cartagena AJ, Banta AB, Sathyan N, Ross W, Gourse RL, Campbell EA, 
            Darst SA
  TITLE     Structural basis for transcription activation by Crl through 
            tethering of sigma(S) and RNA polymerase.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Sep 17;116(38):18923-18927. doi: 
            10.1073/pnas.1910827116. Epub 2019 Sep 4.
  PUBMED    31484766
REFERENCE   2  (bases 1 to 15713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1910827116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-09-17- 17"; volume: "116"; issue: "38"; pages: 
            "18923-18927"
COMMENT     Sequence Label: pAJ1
FEATURES             Location/Qualifiers
     source          1..15713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9399..9422
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9432..9461
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10285..10296
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10406..10561
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             10592..10867
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     promoter        10909..11013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11014..11082,11083..11874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11232..11251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(11916..12429)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12111..12132)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12323..12342
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      12540..13128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13029..13048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13282..13299
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13321..13340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13481..13503
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(13497..13688)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(14245..15327)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     15289..15308
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(15328..15405)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     15611..15630
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gcagggttct gtgacagagt ttctaaaacc
      121 gcgcctggtt gatatcgagc aagtgagttc gacgcacgcc aaggtgaccc ttgagccttt
      181 agagcgtggc tttggccata ctctgggtaa cgcactgcgc cgtattctgc tctcatcgat
      241 gccgggttgc gcggtgaccg aggttgagat tgatggtgta ctacatgagt acagcacaaa
      301 agaaggcgtt caggaagata tcctggaaat cctgctcaac ctgaaagggc tggcggtgag
      361 agttcagggc aaagatgaag ttattcttac cttgaataaa tctggcattg gccctgtgac
      421 tgcagccgat atcacccacg acggtgatgt cgaaatcgtc aagccgcagc acgtgatctg
      481 ccacctgacc gatgagaacg cgtctattag catgcgtatc aaagttcagc gcggtcgtgg
      541 ttatgtgccg gcttctaccc gaattcattc ggaagaagat gagcgcccaa tcggccgtct
      601 gctggtcgac gcatgctaca gccctgtgga gcgtattgcc tacaatgttg aagcagcgcg
      661 tgtagaacag cgtaccgacc tggacaagct ggtcatcgaa atggaaacca acggcacaat
      721 cgatcctgaa gaggcgattc gtcgtgcggc aaccattctg gctgaacaac tggaggcttt
      781 cgttgactta cgtgatgtac gtcagcctga agtgaaagaa gagaaaccag agttcgatcc
      841 gatcctgctg cgccctgttg acgatctgga attgactgtc cgctctgcta actgccttaa
      901 agcagaagct atccactata tcggtgatct ggtacagcgt accgaggttg agctccttaa
      961 aacgcctaac cttggtaaaa aatctcttac tgagattaaa gacgtgctgg cttcccgtgg
     1021 actgtctctg ggcatgcgcc tggaaaactg gccaccggca agcatcgctg acgagtaacc
     1081 ggatccccga tccgtggact tgtcagcgac tgaggaaaca gaccatggat ccccgatccg
     1141 tcgacttgtc agcgagctga ggaaccctat ggtttactcc tataccgaga aaaaacgtat
     1201 tcgtaaggat tttggtaaac gtccacaagt tctggatgta ccttatctcc tttctatcca
     1261 gcttgactcg tttcagaaat ttatcgagca agatcctgaa gggcagtatg gtctggaagc
     1321 tgctttccgt tccgtattcc cgattcagag ctacagcggt aattccgagc tgcaatacgt
     1381 cagctaccgc cttggcgaac cggtgtttga cgtccaggaa tgtcaaatcc gtggcgtgac
     1441 ctattccgca ccgctgcgcg ttaaactgcg tctggtgatc tatgagcgcg aagcgccgga
     1501 aggcaccgta aaagacatta aagaacaaga agtctacatg ggcgaaattc cgctcatgac
     1561 agacaacggt acctttgtta tcaacggtac tgagcgtgtt atcgtttccc agctgcaccg
     1621 tagtccgggc gtcttctttg actccgacaa aggtaaaacc cactcttcgg gtaaagtgct
     1681 gtataacgcg cgtatcatcc cttaccgtgg ttcctggctg gacttcgaat tcgatccgaa
     1741 ggacaacctg ttcgtacgta tcgaccgtcg ccgtaaactg cctgcgacca tcattctgcg
     1801 cgccctgaac tacaccacag agcagatcct cgacctgttc tttgaaaaag ttatctttga
     1861 aatccgtgat aacaagctgc agatggaact ggtgccggaa cgcctgcgtg gtgaaaccgc
     1921 atcttttgac atcgaagcta acggtaaagt gtacgtagaa aaaggccgcc gtatcactgc
     1981 gcgccacatt cgccagctgg aaaaagacga cgtcaaactg atcgaagtcc cggttgagta
     2041 catcgcaggt aaagtggttg ctaaagacta tattgatgag tctaccggcg agctgatctg
     2101 cgcagcgaac atggagctga gcctggatct gctggctaag ctgagccagt ctggtcacaa
     2161 gcgtatcgaa acgctgttca ccaacgatct ggatcacggc ccatatatct ctgaaacctt
     2221 acgtgtcgac ccaactaacg accgtctgag cgcactggta gaaatctacc gcatgatgcg
     2281 ccctggcgag ccgccgactc gtgaagcagc tgaaagcctg ttcgagaacc tgttcttctc
     2341 cgaagaccgt tatgacttgt ctgcggttgg tcgtatgaag ttcaaccgtt ctctgctgcg
     2401 cgaagaaatc gaaggttccg gtatcctgag caaagacgac atcattgatg ttatgaaaaa
     2461 gctcatcgat atccgtaacg gtaaaggcga agtcgatgat atcgaccacc tcggcaaccg
     2521 tcgtatccgt tccgttggcg aaatggcgga aaaccagttc cgcgttggcc tggtacgtgt
     2581 agagcgtgcg gtgaaagagc gtctgtctct gggcgatctg gataccctga tgccacagga
     2641 tatgatcaac gccaagccga tttccgcagc agtgaaagag ttcttcggtt ccagccagct
     2701 gtctcagttt atggaccaga acaacccgct gtctgagatt acgcacaaac gtcgtatctc
     2761 cgcactcggc ccaggcggtc tgacccgtga acgtgcaggc ttcgaagttc gagacgtaca
     2821 cccgactcac tacggtcgcg tatgtccaat cgaaacccct gaaggtccga acatcggtct
     2881 gatcaactct ctgtccgtgt acgcacagac taacgaatac ggcttccttg agactccgta
     2941 tcgtaaagtg accgacggtg ttgtaactga cgaaattcac tacctgtctg ctatcgaaga
     3001 aggcaactac gttatcgccc aggcgaactc caacttggat gaagaaggcc acttcgtaga
     3061 agacctggta acttgccgta gcaaaggcga atccagcttg ttcagccgcg accaggttga
     3121 ctacatggac gtatccaccc agcaggtggt atccgtcggt gcgtccctga tcccgttcct
     3181 ggaacacgat gacgccaacc gtgcattgat gggtgcgaac atgcaacgtc aggccgttcc
     3241 gactctgcgc gctgataagc cgctggttgg tactggtatg gaacgtgctg ttgccgttga
     3301 ctccggtgta actgcggtag ctaaacgtgg tggtgtcgtt cagtacgtgg atgcttcccg
     3361 tatcgttatc aaagttaacg aagacgagat gtatccgggt gaagcaggta tcgacatcta
     3421 caacctgacc aaatacaccc gttctaacca gaacacctgt atcaaccaga tgccgtgtgt
     3481 gtctctgggt gaaccggttg aacgtggcga cgtgctggca gacggtccgt ccaccgacct
     3541 cggtgaactg gcgcttggtc agaacatgcg cgtagcgttc atgccgtgga atggttacaa
     3601 cttcgaagac tccatcctcg tatccgagcg tgttgttcag gaagaccgtt tcaccaccat
     3661 ccacattcag gaactggcgt gtgtgtcccg tgacaccaag ctgggtccgg aagagatcac
     3721 cgctgacatc ccgaacgtgg gtgaagctgc gctctccaaa ctggatgaat ccggtatcgt
     3781 ttacattggt gcggaagtga ccggtggcga cattctggtt ggtaaggtaa cgccgaaagg
     3841 tgaaactcag ctgaccccag aagaaaaact gctgcgtgcg atcttcggtg agaaagcctc
     3901 tgacgttaaa gactcttctc tgcgcgtacc aaacggtgta tccggtacgg ttatcgacgt
     3961 tcaggtcttt actcgcgatg gcgtagaaaa agacaaacgt gcgctggaaa tcgaagaaat
     4021 gcagctcaaa caggcgaaga aagacctgtc tgaagaactg cagatcctcg aagcgggtct
     4081 gttcagccgt atccgtgctg tgctggtagc cggtggcgtt gaagctgaga agctcgacaa
     4141 actgccgcgc gatcgctggc tggagctggg cctgacagac gaagagaaac aaaatcagct
     4201 ggaacagctg gctgagcagt atgacgaact gaaacacgag ttcgagaaga aactcgaagc
     4261 gaaacgccgc aaaatcaccc agggcgacga tctggcaccg ggcgtgctga agattgttaa
     4321 ggtatatctg gcggttaaac gccgtatcca gcctggtgac aagatggcag gtcgtcacgg
     4381 taacaagggt gtaatttcta agatcaaccc gatcgaagat atgccttacg atgaaaacgg
     4441 tacgccggta gacatcgtac tgaacccgct gggcgtaccg tctcgtatga acatcggtca
     4501 gatcctcgaa acccacctgg gtatggctgc gaaaggtatc ggcgacaaga tcaacgccat
     4561 gctgaaacag cagcaagaag tcgcgaaact gcgcgaattc atccagcgtg cgtacgatct
     4621 gggcgctgac gttcgtcaga aagttgacct gagtaccttc agcgatgaag aagttatgcg
     4681 tctggctgaa aacctgcgca aaggtatgcc aatcgcaacg ccggtgttcg acggtgcgaa
     4741 agaagcagaa attaaagagc tgctgaaact tggcgacctg ccgacttccg gtcagatccg
     4801 cctgtacgat ggtcgcactg gtgaacagtt cgagcgtccg gtaaccgttg gttacatgta
     4861 catgctgaaa ctgaaccacc tggtcgacga caagatgcac gcgcgttcca ccggttctta
     4921 cagcctggtt actcagcagc cgctgggtgg taaggcacag ttcggtggtc agcgtttcgg
     4981 ggagatggaa gtgtgggcgc tggaagcata cggcgcagca tacaccctgc aggaaatgct
     5041 caccgttaag tctgatgacg tgaacggtcg taccaagatg tataaaaaca tcgtggacgg
     5101 caaccatcag atggagccgg gcatgccaga atccttcaac gtattgttga aagagattcg
     5161 ttcgctgggt atcaacatcg aactggaaga cgagtaattc tcgctcaaac aggtcactgc
     5221 tgtcgggtta aaacccggca gcggattgtg ctaactccga cgggagcaaa tccgtgaaag
     5281 atttattaaa gtttctgaaa gcgcagacta aaaccgaaga gtttgatgcg atcaaaattg
     5341 ctctggcttc gccagacatg atccgttcat ggtctttcgg tgaagttaaa aagccggaaa
     5401 ccatcaacta ccgtacgttc aaaccagaac gtgacggcct tttctgcgcc cgtatctttg
     5461 ggccggtaaa agattacgag tgcctgtgcg gtaagtacaa gcgcctgaaa caccgtggcg
     5521 tcatctgtga gaagtgcggc gttgaagtga cccagactaa agtacgccgt gagcgtatgg
     5581 gccacatcga actggcttcc ccgactgcgc acatctggtt cctgaaatcg ctgccgtccc
     5641 gtatcggtct gctgctcgat atgccgctgc gcgatatcga acgcgtactg tactttgaat
     5701 ccggttctat gggggcggaa gcaatccagg ctctgctgaa gagcatggat ctggagcaag
     5761 agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc cgaaaccaag cgtaaaaagc
     5821 tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc tggtaacaaa ccagagtgga
     5881 tgatcctgac cgttctgccg gtactgccgc cagatctgcg tccgctggtt ccgctggatg
     5941 gtggtcgttt cgcgacttct gacctgaacg atctgtatcg tcgcgtcatt aaccgtaaca
     6001 accgtctgaa acgtctgctg gatctggctg cgccggacat catcgtacgt aacgaaaaac
     6061 gtatgctgca ggaagcggta gacgccctgc tggataacgg tcgtcgcggt cgtgcgatca
     6121 ccggttctaa caagcgtcct ctgaaatctt tggccgacat gatcaaaggt aaacagggtc
     6181 gtttccgtca gaacctgctc ggtaagcgtg ttgactactc cggtcgttct gtaatcaccg
     6241 taggtccata cctgcgtctg catcagtgcg gtctgccgaa gaaaatggca ctggagctgt
     6301 tcaaaccgtt catctacggc aagctggaac tgcgtggtct tgctaccacc attaaagctg
     6361 cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga tatcctggac gaagttatcc
     6421 gcgaacaccc ggtactgctg aaccgtgcac cgactctgca ccgtctgggt atccaggcat
     6481 ttgaaccggt actgatcgaa ggtaaagcta tccagctgca cccgctggtt tgtgcggcat
     6541 ataacgccga cttcgatggt gaccagatgg ctgttcacgt accgctgacg ctggaagccc
     6601 agctggaagc gcgtgcgctg atgatgtcta ccaacaacat cctgtccccg gcgaacggcg
     6661 aaccaatcat cgttccgtct caggacgttg tactgggtct gtactacatg acccgtgact
     6721 gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc gaaagaagca gaacgtctgt
     6781 atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt gcgtatcacc gagtatgaaa
     6841 aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa agacacgact gttggccgtg
     6901 ccattctgtg gatgattgta ccgaaaggtc tgccttactc catcgtcaac caggcgctgg
     6961 gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg cattctcggt ctgaaaccga
     7021 ccgttatttt tgcggaccag atcatgtaca ccggcttcgc ctatgcagcg cgttctggtg
     7081 catctgttgg tatcgatgac atggtcatcc cggagaagaa acacgaaatc atctccgagg
     7141 cagaagcaga agttgctgaa attcaggagc agttccagtc tggtctggta actgcgggcg
     7201 aacgctacaa caaagttatc gatatctggg ctgcggcgaa cgatcgtgta tccaaagcga
     7261 tgatggataa cctgcaaact gaaaccgtga ttaaccgtga cggtcaggaa gagaagcagg
     7321 tttccttcaa cagcatctac atgatggccg actccggtgc gcgtggttct gcggcacaga
     7381 ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc ggatggctcc atcatcgaaa
     7441 cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact ccagtacttc atctccaccc
     7501 acggtgctcg taaaggtctg gcggataccg cactgaaaac tgcgaactcc ggttacctga
     7561 ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac cgaagacgat tgtggtaccc
     7621 atgaaggtat catgatgact ccggttatcg agggtggtga cgttaaagag ccgctgcgcg
     7681 atcgcgtact gggtcgtgta actgctgaag acgttctgaa gccgggtact gctgatatcc
     7741 tcgttccgcg caacacgctg ctgcacgaac agtggtgtga cctgctggaa gagaactctg
     7801 tcgacgcggt taaagtacgt tctgttgtat cttgtgacac cgactttggt gtatgtgcgc
     7861 actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa caagggtgaa gcaatcggtg
     7921 ttatcgcggc acagtccatc ggtgaaccgg gtacacagct gaccatgcgt acgttccaca
     7981 tcggtggtgc ggcatctcgt gcggctgctg aatccagcat ccaagtgaaa aacaaaggta
     8041 gcatcaagct cagcaacgtg aagtcggttg tgaactccag cggtaaactg gttatcactt
     8101 cccgtaatac tgaactgaaa ctgatcgacg aattcggtcg tactaaagaa agctacaaag
     8161 taccttacgg tgcggtactg gcgaaaggcg atggcgaaca ggttgctggc ggcgaaaccg
     8221 ttgcaaactg ggacccgcac accatgccgg ttatcaccga agtaagcggt tttgtacgct
     8281 ttactgacat gatcgacggc cagaccatta cgcgtcagac cgacgaactg accggtctgt
     8341 cttcgctggt ggttctggat tccgcagaac gtaccgcagg tggtaaagat ctgcgtccgg
     8401 cactgaaaat cgttgatgct cagggtaacg acgttctgat cccaggtacc gatatgccag
     8461 cgcagtactt cctgccgggt aaagcgattg ttcagctgga agatggcgta cagatcagct
     8521 ctggtgacac cctggcgcgt attccgcagg aatccggcgg taccaaggac atcaccggtg
     8581 gtctgccgcg cgttgcggac ctgttcgaag cacgtcgtcc gaaagagccg gcaatcctgg
     8641 ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa aggtaaacgt cgtctggtta
     8701 tcaccccggt agacggtagc gatccgtacg aagagatgat tccgaaatgg cgtcagctca
     8761 acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat ttccgacggt ccggaagcgc
     8821 cgcacgacat tctgcgtctg cgtggtgttc atgctgttac tcgttacatc gttaacgaag
     8881 tacaggacgt ataccgtctg cagggcgtta agattaacga taaacacatc gaagttatcg
     8941 ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg tagctccgac ttcctggaag
     9001 gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg cgaactggaa gcgaacggca
     9061 aagtgggtgc aacttactcc cgcgatctgc tgggtatcac caaagcgtct ctggcaaccg
     9121 agtccttcat ctccgcggca tcgttccagg agaccactcg cgtgctgacc gaagcagccg
     9181 ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa cgttatcgtg ggtcgtctga
     9241 tcccggcagg taccggttac gcgtaccacc aggatcgtat gcgtcgccgt gctgcgggtg
     9301 aagctccggc tgcaccgcag gtgactgcag aagacgcatc tgccagcctg gcagaactgc
     9361 tgaacgcagg tctgggcggt tctgataacg agctcgagct ggaagttctg ttccaggggc
     9421 ccagcagcgg ccatcaccat caccatcatc accatcacca ttaaaagctt attttgagtg
     9481 gactattgag gccagagatc tttctctgtt gggttcccat gttcgtaaag ctacctacca
     9541 gacttacgct ccaattcttt atgagaatga ccactttttc gactacatgc aaaaaagtaa
     9601 gtttcatctc accattgaag gtccaaaagt acttgcttat ttacttggtt tatggattgg
     9661 tgatggattg tctgacaggg caactttttc ggttgattcc agagatactt ctttgatgga
     9721 acgtgttact gaatatgctg aaaagttgaa tttgtgcgcc gagtataagg acagaaaaga
     9781 accacaagtt gccaaaactg ttaatttgta ctctaaagtt gtcagaggta atggtattcg
     9841 caataatctt aatactgaga atccattatg ggacgctatt gttggcttag gattcttgaa
     9901 ggacggtgtc aaaaatattc cttctttctt gtctacggac aatatcggta ctcgtgaaac
     9961 atttcttgct ggtctaattg attctgatgg ctatgttact gatgagcatg gtattaaagc
    10021 aacaataaag acaattcata cttctgtcag agatggtttg gtttcccttg ctcgttcttt
    10081 aggcttagta gtctcggtta acgcagaacc tgctaaggtt gacatgaatg tcaccaaaca
    10141 taaaattagt tatgctattt atatgtctgg tggagatgtt ttgcttaacg ttctttcgaa
    10201 gtgtgccggc tctaaaaaat tcaggcctgc tcccgccgct gcttttgcac gtgagtgccg
    10261 cggattttat ttcgagttac aagaattgaa ggaagacgat tattatggga ttactttatc
    10321 tgatgattct gatcatcagt ttttgcttgg atcccaggtt gtcgtccatg catgcggtgg
    10381 cctgaccggt ctgaactcag gcctcacgac aaatcctggt gtatccgctt ggcaggtcaa
    10441 cacagcttat actgcgggac aattggtcac atataacggc aagacgtata aatgtttgca
    10501 gccccacacc tccttggcag gatgggaacc atccaacgtt cctgccttgt ggcagcttca
    10561 atgactgcag acttaagaag gagattaatg tatggcacgc gtaactgttc aggacgctgt
    10621 agagaaaatt ggtaaccgtt ttgacctggt actggtcgcc gcgcgtcgcg ctcgtcagat
    10681 gcaggtaggc ggaaaggatc cgctggtacc ggaagaaaac gataaaacca ctgtaatcgc
    10741 gctgcgcgaa atcgaagaag gtctgatcaa caaccagatc ctcgacgttc gcgaacgcca
    10801 ggaacagcaa gagcaggaag ccgctgaatt acaagccgtt accgctattg ctgaaggtcg
    10861 tcgttaatcc ggataactac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
    10921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    10981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    11041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    11101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    11161 aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat gatgagcact
    11221 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
    11281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    11341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    11401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    11461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    11521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    11581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    11641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    11701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    11761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    11821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    11881 gaccaagttt actcatatat actttagatt gatttacccc ggttgataat cagaaaagcc
    11941 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
    12001 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
    12061 aaatccctta taaatcaaaa gaatagcccg agatagggtt gagtgttgtt ccagtttgga
    12121 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
    12181 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc
    12241 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
    12301 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
    12361 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
    12421 agggcgcgta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    12481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    12541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    12601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    12661 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    12721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    12781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    12841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    12901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    12961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    13021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    13081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    13141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    13201 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    13261 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagctatggt gcactctcag
    13321 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
    13381 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    13441 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    13501 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
    13561 tcgtgcagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
    13621 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
    13681 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
    13741 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
    13801 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
    13861 ggtcaatgcc agccgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg
    13921 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
    13981 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
    14041 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
    14101 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
    14161 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
    14221 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    14281 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
    14341 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
    14401 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
    14461 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
    14521 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
    14581 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
    14641 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
    14701 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
    14761 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
    14821 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
    14881 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
    14941 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
    15001 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
    15061 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
    15121 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
    15181 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
    15241 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
    15301 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
    15361 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct
    15421 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
    15481 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
    15541 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
    15601 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
    15661 tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
//
