LOCUS       sequence-27600-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes both 
            mCherry and shRNA to be recombined out of the construct, turning OFF
            shRNA expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, 
            Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, 
            Ramalho-Santos M
  TITLE     Chd1 regulates open chromatin and pluripotency of embryonic stem 
            cells.
  JOURNAL   Nature. 2009 Aug 13;460(7257):863-8. doi: 10.1038/nature08212. Epub 
            2009 Jul 8.
  PUBMED    19587682
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08212"; journalName: "Nature"; date: "2009-08-13- 
            13"; volume: "460"; issue: "7257"; pages: "863-8"
COMMENT     Sequence Label: pSicoR-mCh-empty
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(161..364)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(190..210)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        365..668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgn tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgn ttatcctcct cgcccttgct caccatggtg
      121 gcgaccggta gcgctagcgg atctgacggt tcactaaacc agctctgctt atatagacct
      181 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      241 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      301 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
      361 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
      421 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
      481 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
      541 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
      601 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
      661 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
      721 aataactaat gcatggcggt aatacggtta tccacgcggc cgcctatgga tcctagtact
      781 cgaggtcacg cgcgt
//
