LOCUS       sequence-276007-       13392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            C-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13392)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-beta-tubulin_SNAP
FEATURES             Location/Qualifiers
     source          1..13392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(82..99)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    88..199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2711..4636)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5071..5089)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5157..5261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5262..5330,5331..6122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5480..5499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6293..6881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6782..6801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7070..7090)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7088..7385
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7462..8061
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7462..7481)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7958..7978
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8152..8286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8424..8443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8720..8981
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9098..9670
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9678..9697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9725..9744
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9725..9743
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9754..10374
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9827..9846)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10416..10537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10592..10971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10972..11175
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11126..11146
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11177..11195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11198..11216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11226..11250
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11353..11377
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12719..12743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12757..12780
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             13333..13350
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             13351..13374
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgctcgagtc wmgnwgrksm crwktannga ggcgcgcccg gccgctcgag tctagagggc
       61 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      121 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      181 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
      241 tggggcagga cagcaagggg gaggattggg aagacaatag caggctagag gatcgatccc
      301 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
      361 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
      421 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
      481 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
      541 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
      601 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
      661 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
      721 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
      781 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
      841 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
      901 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
      961 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     1021 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     1081 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     1141 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     1201 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     1261 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     1321 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     1381 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     1441 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     1501 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     1561 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     1621 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     1681 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     1741 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     1801 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     1861 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     1921 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     1981 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     2041 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     2101 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     2161 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     2221 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     2281 gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg
     2341 agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc
     2401 cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc
     2461 gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg
     2521 agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac
     2581 gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg
     2641 ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta
     2701 acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt
     2761 catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca
     2821 aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct
     2881 ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta
     2941 aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc
     3001 caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa
     3061 gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac
     3121 cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagctc
     3181 ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca cgatgctttc
     3241 caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt ccagtgcttg
     3301 ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc acgtcaggct
     3361 caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct acagggtgga
     3421 aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat gatgactgac
     3481 tactgggact cctgggcctc ttttctccac gtccacgacc tctccccctg gctctttcac
     3541 gacttccccc cctggctctt tcacgtcctc taccccggcg gcctccacta cctcctcgac
     3601 cccggcctcc actacctcct cgaccccggc ctccactgcc tcctcgaccc cggcctccac
     3661 ctcctgctcc tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     3721 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     3781 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     3841 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
     3901 ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc
     3961 ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc
     4021 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
     4081 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4141 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     4201 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     4261 ctcctgcccc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     4321 ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt
     4381 gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat
     4441 gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc
     4501 ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg
     4561 agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg
     4621 gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg
     4681 ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc
     4741 ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc
     4801 tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt
     4861 gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg
     4921 gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact
     4981 ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg
     5041 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5101 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     5521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     5821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6421 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6901 ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct
     6961 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     7021 taatggggaa ggccatccag cctcgcgtcg aactagatga tccggctgtg gaatgtgtgt
     7081 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7141 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7201 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7261 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7321 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7381 tttggagggt gaccgccacg accggtgccg ccaccatccc ctgacccacg cccctgaccc
     7441 ctcacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7501 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7561 cgccacaccg tcgaccccga ccgccacatc gaacgcgtca ccgagctgca agaactcttc
     7621 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7681 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7741 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7801 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7861 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7921 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7981 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8041 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     8101 gcacgacccg gtccgacgcg gcccgacggg tccgaggggg gtcgacctcg aaacttgttt
     8161 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8221 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8281 tggatctgat ccgaacccct tcctcgacca attctcatgt ttgacagctt atcatcgcag
     8341 atccgggcaa cgttgttgcc attgctcgac caattctcat gtttgacagc ttatcatcgc
     8401 agatccgtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8461 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     8521 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     8581 ctgcttcgcg atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg
     8641 cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc
     8701 ttttgcatag ggagggggaa atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     8761 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     8821 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     8881 ttggacgaac cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata
     8941 caataaacgc catttgacca ttcaccacat tggtgtgcac ctccaagctg gccggggatc
     9001 ctctagggcc tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt
     9061 ttgcaaaaag ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg
     9121 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     9181 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     9241 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     9301 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     9361 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     9421 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     9481 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat
     9541 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     9601 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt
     9661 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     9721 attgtaatac gactcactat agggcgaatt gatatgtcta gattagataa aagtaaagtg
     9781 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
     9841 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg
     9901 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct
     9961 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct
    10021 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag
    10081 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat
    10141 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa
    10201 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta
    10261 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc
    10321 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac
    10381 agcggatccc gggaattcag atcttattaa agcagaactt gtttattgca gcttataatg
    10441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg aatccatggc
    10561 gcagaactgg taggtatgga agatccgcgt tgacattgat tattgactag ttattaatag
    10621 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    10681 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    10741 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    10801 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    10861 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    10921 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    10981 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    11041 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    11101 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    11161 tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt
    11221 cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
    11281 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt
    11341 ggtaccgcca tcacaagttt gtacaaaaaa gcaggcttaa ccaccatgag ggaaatcgtg
    11401 cacatccagg ctggtcagtg tggcaaccag atcggtgcca agttctggga ggtgatcagt
    11461 gatgaacatg gcatcgaccc caccggcacc taccacgggg acagcgacct gcagctggac
    11521 cgcatctctg tgtactacaa tgaagccaca ggtggcaaat atgttcctcg tgccatcctg
    11581 gtggatctag aacctgggac catggactct gttcgctcag gtccttttgg ccagatcttt
    11641 agaccagaca actttgtatt tggtcagtct ggggcaggta acaactgggc caaaggccac
    11701 tacacagagg gcgccgagct ggtcgattct gtcctggatg tggtacggaa ggaggcagag
    11761 agctgtgact gcctgcaggg cttccagctg acccactcac tgggcggggg cacaggctct
    11821 ggaatgggca ctctccttat cagcaagatc cgagaagaat accctgatcg catcatgaat
    11881 accttcagtg tggtgccttc acccaaagtg tctgacaccg tggtcgagcc ctacaatgcc
    11941 accctctccg tccatcagtt ggtagagaat actgatgaga cctattgcat tgacaacgag
    12001 gccctctatg atatctgctt ccgcactctg aagctgacca caccaaccta cggggatctg
    12061 aaccaccttg tctcagccac catgagtggt gtcaccacct gcctccgttt ccctggccag
    12121 ctcaatgctg acctccgcaa gttggcagtc aacatggtcc ccttcccacg tctccatttc
    12181 tttatgcctg gctttgcccc tctcaccagc cgtggaagcc agcagtatcg agctctcaca
    12241 gtgccggaac tcacccagca ggtcttcgat gccaagaaca tgatggctgc ctgtgacccc
    12301 cgccacggcc gatacctcac cgtggctgct gtcttccgtg gtcggatgtc catgaaggag
    12361 gtcgatgagc agatgcttaa cgtgcagaac aagaacagca gctactttgt ggaatggatc
    12421 cccaacaatg tcaagacagc cgtctgtgac atcccacctc gtggcctcaa gatggcagtc
    12481 accttcattg gcaatagcac agccatccag gagctcttca agcgcatctc ggagcagttc
    12541 actgccatgt tccgccggaa ggccttcctc cactggtaca caggcgaggg catggacgag
    12601 atggagttca ccgaggctga gagcaacatg aacgacctcg tctctgagta tcagcagtac
    12661 caggatgcca ccgcagaaga ggaggaggat ttcggtgagg aggccgaaga ggaggcctac
    12721 ccagctttct tgtacaaagt ggtgatggca gctagcctgg aggtgctgtt ccagggcccc
    12781 ttcgaaatgg acaaggattg tgaaatgaaa cgcaccacac tggacagccc tttggggaag
    12841 ctggagctgt ctggttgtga gcagggtctg cacgaaataa tttttctggg caaggggacg
    12901 tctgcagctg atgccgtgga ggtcccagcc cccgctgcgg ttctcggagg tccggagccc
    12961 ctgatgcagg ctacagcctg gctgaatgcc tatttccacc agcccgaggc tatcgaagag
    13021 ttccccgtgc cggctcttca ccatcccgtt ttccagcaag agtcgttcac cagacaggtg
    13081 ttatggaagc tgctgaaggt tgtgaaattc ggagaagtga tttcttactc tcacttagca
    13141 gccctggcag gcaaccccgc agccacggca gcagtgaaga cggcactgag tggcaatcct
    13201 gtccctatcc tgatcccgtg ccacagagtg gtccaggggg atttggatgt gggcggttac
    13261 gagggtggac tggccgtgaa ggaatggctt ctggcccatg aaggccaccg gttggggaag
    13321 ccaggcttgg gacaccacca ccaccaccac gattataaag acgatgatga caagtaatga
    13381 ggcgcgcccg gc
//
