LOCUS       sequence-27601-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, 
            Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, 
            Ramalho-Santos M
  TITLE     Chd1 regulates open chromatin and pluripotency of embryonic stem 
            cells.
  JOURNAL   Nature. 2009 Aug 13;460(7257):863-8. doi: 10.1038/nature08212. Epub 
            2009 Jul 8.
  PUBMED    19587682
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08212"; journalName: "Nature"; date: "2009-08-13- 
            13"; volume: "460"; issue: "7257"; pages: "863-8"
COMMENT     Sequence Label: pSicoR-mCh-Chd1i4
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..143)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        400..703
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat
       61 ctgttctttt taatactagc tacattttac atgataggct tggatttcta taacttcgta
      121 tagcatacat tatacgaagt tatacatgtc acaaaaggaa actcacccta actgtaaagt
      181 aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgtttg tgctactaca
      241 accatttatt caagagataa atggttgtag tagcactttt ttctcgagta ctaggatcca
      301 ttaggcggcc gcgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
      361 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      421 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      481 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      541 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      601 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      661 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
//
