LOCUS       sequence-276016-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for phycocyanobilin (PCB) synthesis in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, Aoki K
  TITLE     Improvement of Phycocyanobilin Synthesis for Genetically Encoded 
            Phytochrome-Based Optogenetics.
  JOURNAL   ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 
            10.1021/acschembio.0c00477. Epub 2020 Nov 9.
  PUBMED    33164485
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.0c00477"; journalName: "ACS Chem Biol"; date: 
            "2020-11-20- 20"; volume: "15"; issue: "11"; pages: "2896-2906"
COMMENT     Sequence Label: pT2Apuro-TRE-synPCB2.0
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(2242..2253,2254..2441)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        2496..2810
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2504..2522
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2540..2558
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2575..2593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2611..2629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2647..2665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2682..2700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2718..2736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2783..2807
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2824..2898
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3345..3354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3634..3657
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3667..3723
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3727..3801
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3832..3858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4594..4650
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4651..4725
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     misc_feature    6067..6616
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6211..6228)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6438..6457
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6619..7218
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6619..6638)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7115..7135
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7272..7406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7296..7315)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7350..7369
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7528..7690,7691..7702)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7732..7751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7733..7751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7761..7778)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7761..7777)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7770..7792)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7919..8346
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8005..8024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8215..8236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcggatctg ctgggcttgc
     2221 tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact
     2281 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
     2341 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
     2401 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggccctct agatgcatgc
     2461 tcgacattga ttattgacta gttttggggg gggtcgagtt tactccctat cagtgataga
     2521 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     2581 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2641 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     2701 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     2761 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattc
     2821 accatgtcag tgctgactcc actgctgctg aggggcctga ctgggtccgc acgccgcctg
     2881 cctgtcccaa gagccaaaat ccatagcctg ggcgggtctg gaggcatgag tctgaggcag
     2941 caccagcatc ctctgatcca gcgcctggcc gatcgaatcg aggcaatttg gcaggccttc
     3001 tttcccctgg caccttacgc cctgccagaa gacctgggct atgtggaggg aaaactggag
     3061 ggcgaacgcc tgacaatcga gaaccactgc tatcaggccc ccccttttcg gaagctgcat
     3121 ctggaactgg ctagagtggg cgagtctctg gacattctgc actgcgtcat gtttcccgag
     3181 cctagatacg atctgcccat gttcggatgt gacctggtgg ggggacgcgg acagatcagt
     3241 gccgctattg tggatctgtc accagtcact ggacagctgc cagcagcata cacctgcgct
     3301 ctgaatgcac tgcctaagct gacctttaga cagccaaggg agctgccacc atggggacat
     3361 atcttctctc ccttttgtat cttcattaga cctcagggcg aagccgagga acagcagttc
     3421 ctggatagga ttggggagta tctgactctg cactgccagc tgagccagca ggcagtgcct
     3481 accgaccatc cacaggccgt catcgctgga cagcggcagt attgtcagca gcagcagcag
     3541 aacgataaaa ctcggagagt gctggaaaag gcttttggcg tcccatgggc agagagatac
     3601 atgaccacag tgctgttcga tgtgcctcca gtggactata aagacgatga cgataaaggc
     3661 agcggagcga cgaattttag tctactaaaa caagcgggtg atgtagaaga aaatccgggt
     3721 ccgggcatgt cagttctgac acctctgctg ctgcgtggac tgactggaag cgccaggcgc
     3781 ctgcctgtgc cacgagcaaa aatccactca ctgggcggga gcggaggcat gtacccctat
     3841 gatgtccctg actacgcctc tagaatgact accagcctgg ctacaaagct gagggaaggc
     3901 actaagaaag ctcataccat ggcagagaac gtggggtttg tccggtgttt cctgaaggga
     3961 actgtggaga aaagctccta tagaaagctg gtggcctctc tgtaccacgt ctatagtgct
     4021 atggagcagg aaatggagcg cctgaaagac catccaatct tgggcaaaat ctactttccc
     4081 gaactgaaca ggaagtctag tctggagcgc gatctgacat actatttcgg gtccaattgg
     4141 agggaggaaa ttcccccttc tcccgcaact caggcctacg tggctcgcat ccacgaagtc
     4201 gcaaacaccg cccctgagct gctggtggcc catagttaca cacggtatct gggagacctg
     4261 tcagggggac agatcctgaa gggcattgct gaacgggcaa tgaatctgca ggatggggag
     4321 ggaaccgcct tctacagatt cgaaagtatc tcagacgaga aggccttcaa acagctgtat
     4381 cgacagcggc tggatgaact gcccgtggac gaggctacag cagatagaat tgtggacgag
     4441 gctaacgctg catttggcat gaatatgaag attttccagg aactggaggg caacctgatc
     4501 cgggccattg ggcagctgct gtttaatacc ctgacacgac ggaaacagag aggcagcacc
     4561 gagctggcca cagctgatag atctggctca ggtgcgacga attttagtct actgaaacaa
     4621 gcgggagacg tggaggaaaa ccctggacct atgtccgtcc tgaccccact gctgctgagg
     4681 ggactgacag gatctgcaag aaggctgcca gtgcccagag ccaaaattca ctccctggga
     4741 ggctctggag gcctcgagaa tatttaccga cctaacaatc cttgcattgg gaaagtcatt
     4801 agcaatgagg agctggtgcg agagggggga gaagggactg taaaacatat aatcttcgat
     4861 atatcaggca cggagcttcg gtatcttgag gggcaatcta ttggaatcat accggcgggt
     4921 actgatgcca atggaaaacc gcataaattg cgcctctact ctatagcttc tactaggcac
     4981 ggggatttcc aggatgataa gaccgtctct ttgtgtgtcc gcaggctgga atacaaggat
     5041 aaggaaaccg gagagacaat atatggggta tgttcctcat acctgaatca attgcaacca
     5101 ggagatgaag tgaagataac ggggcctgtt ggtaaggaga tgctcctgtc agatgaccca
     5161 gaagctacaa ttataatgct ggcgactggg actggcatag cgcctttccg agccttcctc
     5221 tggagaatgt tcaaggaaaa caaccccgat tatcaattca aggggcttgc ctggttgttt
     5281 tttggtgtag catacacggc taacatcctt tacaaagatg aactcgaagc gattcaggcg
     5341 cagtacccgg atcattttcg cttgacatac gccatatcta gggaacagaa gacccccgac
     5401 gggggtaaaa tgtatatcca agggcgaata gccgaacatg cagatgaaat ctggcaactg
     5461 ctgcaaaaga aaaatactca cgtctatatg tgtggtctca gaggaatgga gccgggcata
     5521 gacgaagcga tgaccgctgc ggcggcaaag aacggcgccg attggcaaga atttctcaag
     5581 ggtacactta agaaggaggg gcgctggcac gtcgagacgt acggcggatc cggaggttcc
     5641 ggcggctcag gaggttctat ggctacctat aaggtcacgc tggttcgacc agatggtagc
     5701 gagactacca ttgatgtccc tgaagacgaa tatattctgg acgtagctga ggagcaaggc
     5761 ttggatcttc ctttcagctg ccgggctggt gcttgcagca cgtgcgcggg aaaacttctg
     5821 gagggagagg tcgatcagag cgatcagtcc ttcctcgacg atgaccaaat agaaaagggt
     5881 ttcgttctca cttgcgttgc ctacccaaga agcgattgca aaatactcac aaaccaggag
     5941 gaagagctgt acggcggccg catggcttca atgacaggag gacagcagat ggggtaagtc
     6001 gacgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc
     6061 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6121 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6181 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     6241 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     6301 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6361 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6421 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6481 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6541 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6601 gaaaaacacg ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6661 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6721 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6781 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6841 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6901 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6961 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7021 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7081 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7141 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7201 cgcaagcccg gtgcctgagg atccaatgga ggccgccacc gcggtggagc tcgaattaat
     7261 tcatcgatga tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     7321 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     7381 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     7441 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     7501 ctgattatga tcctctagat cagatctttc ttgcttttac ttttacttcc ttaatactca
     7561 agtacaattt taatggagta cttttttact tttactcaag taagattcta gccagatact
     7621 tttactttta attgagtaaa attttcccta agtacttgta ctttcacttg agtaaaattt
     7681 ttgagtactt tttacacctc tgtcaagaac catatgccgg tacccaattc gccctatagt
     7741 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7801 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7861 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     7921 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccacc
     7981 acacccgccg cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8041 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8101 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8161 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8221 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8281 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8341 cgaattttaa caaaatatta acgcttacaa tttaggtgg
//
