LOCUS       sequence-276038-       13067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            N-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..13067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2404..4329)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(4764..4782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4850..4954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4955..5023,5024..5815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5173..5192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5986..6574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6475..6494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6763..6783)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6781..7078
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7155..7754
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7155..7174)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7651..7671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7845..7979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8117..8136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8413..8674
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8791..9363
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9371..9390
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9418..9437
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9418..9436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9447..10067
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9520..9539)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10109..10230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10285..10664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10665..10868
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10819..10839
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10870..10888
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10891..10909
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10919..10943
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11043..11066
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11067..11084
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11634..11657
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11670..11694
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13057..13067,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3541 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     3601 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     3661 ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     3721 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     3781 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3841 tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
     3901 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     3961 ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     4021 tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc
     4081 tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg
     4141 ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc
     4201 ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc
     4261 tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc
     4321 gtcagacatg attcacacta aaagagatca atagacatct ttattagacg acgctcagtg
     4381 aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat
     4441 ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct
     4501 catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct
     4561 gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg
     4621 attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa
     4681 cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca
     4741 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4801 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4921 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4981 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5041 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5101 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5161 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     5221 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5281 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5341 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5401 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5461 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     5521 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5581 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5641 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5701 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5761 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5821 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5881 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6121 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6601 gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
     6661 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
     6721 gaaggccatc cagcctcgcg tcgaactaga tgatccggct gtggaatgtg tgtcagttag
     6781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6901 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7021 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7081 ggtgaccgcc acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa
     7141 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7201 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7261 ccgtcgaccc cgaccgccac atcgaacgcg tcaccgagct gcaagaactc ttcctcacgc
     7321 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7381 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7441 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7501 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7561 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7621 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7681 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7741 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     7801 ccggtccgac gcggcccgac gggtccgagg ggggtcgacc tcgaaacttg tttattgcag
     7861 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7921 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     7981 gatccgaacc ccttcctcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     8041 caacgttgtt gccattgctc gaccaattct catgtttgac agcttatcat cgcagatccg
     8101 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8161 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8221 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     8281 gcgatgtacg ggccagatat acgcgtatct gaggggacta gggtgtgttt aggcgaaaag
     8341 cggggcttcg gttgtacgcg gttaggagtc ccctcaggat atagtagttt cgcttttgca
     8401 tagggagggg gaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8461 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8521 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8581 aaccactgaa ttccgcattg cagagatatt gtatttaagt gcctagctcg atacaataaa
     8641 cgccatttga ccattcacca cattggtgtg cacctccaag ctggccgggg atcctctagg
     8701 gcctctgagc tattccagaa gtagtgaaga ggcttttttg gaggcctagg cttttgcaaa
     8761 aagctccgga tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc
     8821 tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt
     8881 agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac
     8941 tttctactct gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt
     9001 tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca
     9061 gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat attatattgt
     9121 acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg
     9181 catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc
     9241 atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa
     9301 tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta
     9361 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgtaa
     9421 tacgactcac tatagggcga attgatatgt ctagattaga taaaagtaaa gtgattaaca
     9481 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
     9541 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
     9601 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
     9661 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
     9721 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
     9781 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
     9841 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
     9901 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
     9961 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
    10021 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgcg tacagcggat
    10081 cccgggaatt cagatcttat taaagcagaa cttgtttatt gcagcttata atggttacaa
    10141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10201 tggtttgtcc aaactcatca atgtatctta tcatgtctgg tcgaatccat ggcgcagaac
    10261 tggtaggtat ggaagatccg cgttgacatt gattattgac tagttattaa tagtaatcaa
    10321 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
    10381 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
    10441 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    10501 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    10561 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    10621 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    10681 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    10741 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    10801 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    10861 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
    10921 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
    10981 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttaccacca
    11041 tggattataa agacgatgat gacaagcacc accaccacca ccacgacaag gattgtgaaa
    11101 tgaaacgcac cacactggac agccctttgg ggaagctgga gctgtctggt tgtgagcagg
    11161 gtctgcacga aataattttt ctgggcaagg ggacgtctgc agctgatgcc gtggaggtcc
    11221 cagcccccgc tgcggttctc ggaggtccgg agcccctgat gcaggctaca gcctggctga
    11281 atgcctattt ccaccagccc gaggctatcg aagagttccc cgtgccggct cttcaccatc
    11341 ccgttttcca gcaagagtcg ttcaccagac aggtgttatg gaagctgctg aaggttgtga
    11401 aattcggaga agtgatttct tactctcact tagcagccct ggcaggcaac cccgcagcca
    11461 cggcagcagt gaagacggca ctgagtggca atcctgtccc tatcctgatc ccgtgccaca
    11521 gagtggtcca gggggatttg gatgtgggcg gttacgaggg tggactggcc gtgaaggaat
    11581 ggcttctggc ccatgaaggc caccggttgg ggaagccagg cttgggattc gaactggagg
    11641 tgctgttcca gggccccggt accgccatca caagtttgta caaaaaagca ggcttagtgc
    11701 gtgagtgcat ctccatccac gttggccagg ctggtgtcca gattggcaat gcctgctggg
    11761 agctctactg cctggaacac ggcatccagc ccgatggcca gatgccaagt gacaagacca
    11821 ttgggggagg agatgactcc ttcaacacct tcttcagtga gacgggcgct ggcaagcacg
    11881 tgccccgggc tgtgtttgta gacttggaac ccacagtcat tgatgaagtt cgcactggca
    11941 cctaccgcca gctcttccac cctgagcagc tcatcacagg caaggaagat gctgccaata
    12001 actatgcccg agggcactac accattggca aggagatcat tgaccttgtg ttggaccgaa
    12061 ttcgcaagct ggctgaccag tgcaccggtc ttcagggctt cttggttttc cacagctttg
    12121 gtgggggaac tggttctggg ttcacctccc tgctcatgga acgtctctca gttgattatg
    12181 gcaagaagtc caagctggag ttctccattt acccagcacc ccaggtttcc acagctgtag
    12241 ttgagcccta caactccatc ctcaccaccc acaccaccct ggagcactct gattgtgcct
    12301 tcatggtaga caatgaggcc atctatgaca tctgtcgtag aaacctcgat atcgagcgcc
    12361 caacctacac taaccttaac cgccttatta gccagattgt gtcctccatc actgcttccc
    12421 tgagatttga tggagccctg aatgttgacc tgacagaatt ccagaccaac ctggtgccct
    12481 acccccgcat ccacttccct ctggccacat atgcccctgt catctctgct gagaaagcct
    12541 accatgaaca gctttctgta gcagagatca ccaatgcttg ctttgagcca gccaaccaga
    12601 tggtgaaatg tgaccctcgc catggtaaat acatggcttg ctgcctgttg taccgtggtg
    12661 acgtggttcc caaagatgtc aatgctgcca ttgccaccat caaaaccaag cgcagcatcc
    12721 agtttgtgga ttggtgcccc actggcttca aggttggcat caactaccag cctcccactg
    12781 tggtgcctgg tggagacctg gccaaggtac agagagctgt gtgcatgctg agcaacacca
    12841 cagccattgc tgaggcctgg gctcgcctgg accacaagtt tgacctgatg tatgccaagc
    12901 gtgcctttgt tcactggtac gtgggtgagg ggatggagga aggcgagttt tcagaggccc
    12961 gtgaagatat ggctgccctt gagaaggatt atgaggaggt tggtgtggat tctgttgaag
    13021 gagagggtga ggaagaagga gaggaatact aatgataccc agctttc
//
