LOCUS       sequence-276041-       12336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PhyB621-mCherry-HRasCT in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, Aoki K
  TITLE     Improvement of Phycocyanobilin Synthesis for Genetically Encoded 
            Phytochrome-Based Optogenetics.
  JOURNAL   ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 
            10.1021/acschembio.0c00477. Epub 2020 Nov 9.
  PUBMED    33164485
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.0c00477"; journalName: "ACS Chem Biol"; date: 
            "2020-11-20- 20"; volume: "15"; issue: "11"; pages: "2896-2906"
COMMENT     Sequence Label: pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
FEATURES             Location/Qualifiers
     source          1..12336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2592..2906
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2600..2618
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2636..2654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2671..2689
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2707..2725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2743..2761
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2778..2796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2814..2832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2879..2903
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4949..4967)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5217..5236
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5649..6198
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5793..5810)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6020..6039
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6201..6995
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6255..6274)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6865..6884
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7031..7619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7084..7104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7502..7513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8138..8318
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8344..8361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8350..8574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8620..9048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8707..8726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8917..8938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9057..9077)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9062..9391
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9242..9377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9304..9323
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9439..9486
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9505..9879
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10009..10130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10046..10065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10100..10119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10179..10195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10179..10195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10192..10214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10203..10219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10227..10233,10234..10251,10252..10257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10272..10293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10410..10427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10581..11169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10661..10680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11340..12131,12132..12200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11963..11982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12201..12305)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcggggctt
     2581 tgggggggct ggagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     2641 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2701 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     2761 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2821 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2881 ctcgtttagt gaaccgtcag atcgcctgga gaattcacca tggtgtcagg agtgggagga
     2941 agcggaggcg gcagaggagg cgggagagga ggcgaggaag agccaagctc ctctcacact
     3001 cccaacaatc ggagaggggg agagcaggct cagagttcag gcactaagtc cctgaggccc
     3061 cgctctaaca ccgaatccat gtctaaagcc atccagcagt acaccgtgga tgctaggctg
     3121 catgccgtgt ttgaacagtc cggggagtct ggaaagagtt tcgactacag tcagtcactg
     3181 aaaaccacaa cttatgggag ctccgtgccc gagcagcaga ttacagctta cctgagcagg
     3241 atccagaggg gaggatatat tcagcctttt ggatgcatga tcgctgtgga tgagtctagt
     3301 ttccggatca ttgggtactc tgaaaatgcc agagagatgc tgggaatcat gccacagagt
     3361 gtgcccactc tggaaaagcc tgagattctg gccatgggca ccgacgtgcg gtctctgttt
     3421 acatcaagct cctctatcct gctggagcgg gctttcgtgg ccagagaaat cactctgctg
     3481 aacccagtgt ggattcacag caagaatacc ggcaaaccct tttatgccat cctgcatagg
     3541 attgacgtgg gggtggtcat cgatctggaa cctgctcgca ccgaggatcc agccctgagc
     3601 attgctggcg ccgtgcagtc ccagaagctg gctgtgagag ccatcagcca gctgcaggcc
     3661 ctgccaggag gcgacatcaa gctgctgtgc gatacagtgg tggagtccgt gagggacctg
     3721 actggatacg atcgcgtgat ggtgtataag ttccacgaag acgagcatgg cgaagtggtg
     3781 gccgagagca aaagggacga tctggagcct tacatcggcc tgcactatcc tgctaccgat
     3841 attccacagg cctcccgctt cctgtttaag cagaaccggg tgagaatgat cgtggactgt
     3901 aatgctacac cagtgctggt ggtgcaggac gatcggctga cccagagtat gtgcctggtg
     3961 ggatcaacac tgagagcccc ccacggctgt catagccagt acatggctaa catggggtca
     4021 attgccagcc tggctatggc cgtgatcatt aacggcaatg aggacgatgg gagcaatgtg
     4081 gcctccggga ggagttcaat gcgcctgtgg ggactggtgg tgtgccacca tacaagctcc
     4141 cggtgtatcc catttcccct gagatatgct tgcgagtttc tgatgcaggc cttcggcctg
     4201 cagctgaaca tggagctgca gctggccctg cagatgagcg aaaagcgggt gctgagaacc
     4261 cagacactgc tgtgcgacat gctgctgagg gattccccag ctggaatcgt gacccagagc
     4321 ccttccatta tggacctggt gaagtgtgat ggagccgctt tcctgtacca cggcaaatac
     4381 tatccactgg gagtggctcc ttccgaggtg cagatcaagg atgtggtgga atggctgctg
     4441 gctaatcatg ccgactctac aggcctgtcc actgactctc tgggcgatgc tggataccct
     4501 ggagccgctg ccctgggaga tgccgtgtgc ggaatggctg tggcctatat tacaaaaagg
     4561 gacttcctgt tttggttccg cagccacact gctaaggaga tcaaatgggg gggagccaag
     4621 caccatcctg aagataaaga cgatggccag cggatgcatc caagatctag ttttcaggcc
     4681 ttcctggagg tggtgaagtc tcggagtcag ccatgggaaa ccgctgagat ggatgccatc
     4741 cacagtctgc agctgattct gagagactct tttaaggaga gtggaggtag tggaggcggc
     4801 cgcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     4861 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     4921 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4981 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     5041 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     5101 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     5161 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     5221 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     5281 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     5341 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     5401 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5461 gaacgcgccg agggccgcca ctccactctc ggcatggacg agctgtctag aaagctgaac
     5521 cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc ctgactagag
     5581 gatccgttaa ctaacttaag ctagcaacgg tttccctcta gcgggatcaa ttccgccccc
     5641 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     5701 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5761 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5821 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5881 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5941 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     6001 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     6061 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     6121 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     6181 ttgaaaaaca cgataatacc atgattgaac aagatggatt gcatgcaggt tcgccggccg
     6241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6361 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6721 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6961 tcgccttcta tcgccttctt gacgagttct tctgaactag agttaacatc gagggatcaa
     7021 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7081 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7141 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7201 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7261 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7321 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7381 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7441 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7501 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7561 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7621 tcgataccgt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     7681 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     7741 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7801 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     7861 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     7921 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     7981 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     8041 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     8101 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     8161 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8221 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8281 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     8341 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8401 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8461 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8521 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8581 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     8641 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8701 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8761 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8821 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8881 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8941 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9001 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     9061 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9121 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9181 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9241 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9301 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     9361 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     9421 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     9481 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     9541 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     9601 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     9661 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     9721 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     9781 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     9841 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     9901 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9961 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    10021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    10081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    10141 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    10201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10981 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    11041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12301 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
