LOCUS       sequence-276050-        8491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector with CMV promoter driving mutant form of 
            acetyltransferase and CH3 domains fragment of human p300 (EP300) 
            (WY1466-1467AS in AT domain)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Borger DR, DeCaprio JA
  TITLE     Targeting of p300/CREB binding protein coactivators by simian virus 
            40 is mediated through p53.
  JOURNAL   J Virol. 2006 May . 80(9):4292-303.
  PUBMED    16611888
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2006 May . 80(9):4292-303."
COMMENT     Sequence Label: pLB(N)CX AT-CH3-FLAG-HA WY
FEATURES             Location/Qualifiers
     source          1..8491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1869..1878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2883..2906
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2922..2948
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2922..2948
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             3003..3596
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3699..3721)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3807..3947
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3862..3881
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3962..3979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4133..4721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4213..4232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4892..5683,5684..5752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5515..5534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5753..5857)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5925..5943
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             6515..6690
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    6753..6952
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7153..7569
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7499..7521
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7537..7553
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7612..8010
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccccaag ctttgaacca tggcccagaa caggtatcat ttctgtgaga
      721 agtgtttcaa tgagatccaa ggggagagcg tttctttggg ggatgaccct tcccagcctc
      781 aaactacaat aaataaagaa caattttcca agagaaaaaa tgacacactg gatcctgaac
      841 tgtttgttga atgtacagag tgcggaagaa agatgcatca gatctgtgtc cttcaccatg
      901 agatcatctg gcctgctgga ttcgtctgtg atggctgttt aaagaaaagt gcacgaacta
      961 ggaaagaaaa taagttttct gctaaaaggt tgccatctac cagacttggc acctttctag
     1021 agaatcgtgt gaatgacttt ctgaggcgac agaatcaccc tgagtcagga gaggtcactg
     1081 ttagagtagt tcatgcttct gacaaaaccg tggaagtaaa accaggcatg aaagcaaggt
     1141 ttgtggacag tggagagatg gcagaatcct ttccataccg aaccaaagcc ctctttgcct
     1201 ttgaagaaat tgatggtgtt gacctgtgct tctttggcat gcatgttcaa gagtatggct
     1261 ctgactgccc tccacccaac cagaggagag tatacatatc ttacctcgat agtgttcatt
     1321 tcttccgtcc taaatgcttg aggactgcag tctatcatga aatcctaatt ggatatttag
     1381 aatatgtcaa gaaattaggt tacacaacag ggcatatttg ggcatgtcca ccaagtgagg
     1441 gagatgatta tatcttccat tgccatcctc ctgaccagaa gatacccaag cccaagcgac
     1501 tgcaggaagc tagcaaaaaa atgcttgaca aggctgtatc agagcgtatt gtccatgact
     1561 acaaggatat ttttaaacaa gctactgaag atagattaac aagtgcaaag gaattgcctt
     1621 atttcgaggg tgatttctgg cccaatgttc tggaagaaag cattaaggaa ctggaacagg
     1681 aggaagaaga gagaaaacga gaggaaaaca ccagcaatga aagcacagat gtgaccaagg
     1741 gagacagcaa aaatgctaaa aagaagaata ataagaaaac cagcaaaaat aagagcagcc
     1801 tgagtagggg caacaagaag aaacccggga tgcccaatgt atctaacgac ctctcacaga
     1861 aactatatgc caccatggag aagcataaag aggtcttctt tgtgatccgc ctcattgctg
     1921 gccctgctgc caactccctg cctcccattg ttgatcctga tcctctcatc ccctgcgatc
     1981 tgatggatgg tcgggatgcg tttctcacgc tggcaaggga caagcacctg gagttctctt
     2041 cactccgaag agcccagtgg tccaccatgt gcatgctggt ggagctgcac acgcagagcc
     2101 aggaccgctt tgtctacacc tgcaatgaat gcaagcacca tgtggagaca cgctggcact
     2161 gtactgtctg tgaggattat gacttgtgta tcacctgcta taacactaaa aaccatgacc
     2221 acaaaatgga gaaactaggc cttggcttag atgatgagag caacaaccag caggctgcag
     2281 ccacccagag cccaggcgat tctcgccgcc tgagtatcca gcgctgcatc cagtctctgg
     2341 tccatgcttg ccagtgtcgg aatgccaatt gctcactgcc atcctgccag aagatgaagc
     2401 gggttgtgca gcataccaag ggttgcaaac ggaaaaccaa tggcgggtgc cccatctgca
     2461 agcagctcat tgccctctgc tgctaccatg ccaagcactg ccaggagaac aaatgcccgg
     2521 tgccgttctg cctaaacatc aagcagaagc tccggcagca acagctgcag caccgactac
     2581 agcaggccca aatgcttcgc aggaggatgg ccagcatgca gcggactggt gtggttgggc
     2641 agcaacaggg cctcccttcc cccactcctg ccactccaac gacaccaact ggccaacagc
     2701 caaccacccc gcagacgccc cagcccactt ctcagcctca gcctacccct cccaatagca
     2761 tgccacccta cttgcccagg actcaagctg ctggccctgt gtcccagggt aaggcagcag
     2821 gccaggtgac ccctccaacc cctcctcaga ctgctcagcc accccttcca gggcccggag
     2881 gagactacaa ggacgacgat gacaagtcgg ccgctggagg atacccctac gacgtgcccg
     2941 actacgccta ggttaacatc gataaaataa aagattttat ttagtctcca gaaaaagggg
     3001 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     3061 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
     3121 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     3181 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     3241 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
     3301 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     3361 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     3421 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     3481 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     3541 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
     3601 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa
     3661 gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3721 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3781 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     3841 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     3901 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     3961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4561 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     5221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     5521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5881 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     5941 ggccctttcg tcttcaagaa ttcataccag atcaccgaaa actgtcctcc aaatgtgtcc
     6001 ccctcacact cccaaattcg cgggcttctg cctcttagac cactctaccc tattccccac
     6061 actcaccgga gccaaagccg cggcccttcc gtttctttgc ttttgaaaga ccccacccgt
     6121 aggtggcaag ctagcttaag taacgccact ttgcaaggca tggaaaaata cataactgag
     6181 aatagaaaag ttcagatcaa ggtcaggaac aaagaaacag ctgaatacca aacaggatat
     6241 ctgtggtaag cggttcctgc cccggctcag ggccaagaac agatgagaca gctgagtgat
     6301 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcggggcc aagaacagat
     6361 ggtccccaga tgcggtccag ccctcagcag tttctagtga atcatcagat gtttccaggg
     6421 tgccccaagg acctgaaaat gaccctgtac cttatttgaa ctaaccaatc agttcgcttc
     6481 tcgcttctgt tcgcgcgctt ccgctctccg agctcaataa aagagcccac aacccctcac
     6541 tcggcgcgcc agtcttccga tagactgcgt cgcccgggta cccgtattcc caataaagcc
     6601 tcttgctgtt tgcatccgaa tcgtggtctc gctgttcctt gggagggtct cctctgagtg
     6661 attgactacc cacgacgggg gtctttcatt tgggggctcg tccgggattt ggagacccct
     6721 gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg
     6781 tccgattgtc tagtgtctat gtttgatgtt atgcgcctgc gtctgtacta gttagctaac
     6841 tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac
     6901 cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag
     6961 tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa
     7021 cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt
     7081 ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
     7141 tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
     7201 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
     7261 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
     7321 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
     7381 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
     7441 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
     7501 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
     7561 ctaggcgccg gaattccgat ctgatcaaga gacaggatgg ggtcgtttcg catggccaag
     7621 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     7681 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     7741 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     7801 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     7861 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     7921 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     7981 ctgccctctg gttatgtgtg ggagggctaa gcgggactct ggggttcgaa atgaccgacc
     8041 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     8101 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8161 tgctggagtt cttcgcccac cccgggctcg atcccctcgc gagttggttc agctgctgcc
     8221 tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa atccgtcggc atccaggaaa
     8281 ccagcagcgg ctatccgcgc atccatgccc ccgaactgca ggagtgggga ggcacgatgg
     8341 ccgctttggt cgaggcggat ccggccatta gccatattat tcattggtta tatagcataa
     8401 atcaatattg gctattggcc attgcatacg ttgtatccat atcataatat gtacatttat
     8461 attggctcat gtccaacatt accgccatgt t
//
