LOCUS       sequence-276075-        6907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector driving CH3 domain fragment of human 
            p300 (EP300), with N-terminal FLAG tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Borger DR, DeCaprio JA
  TITLE     Targeting of p300/CREB binding protein coactivators by simian virus 
            40 is mediated through p53.
  JOURNAL   J Virol. 2006 May . 80(9):4292-303.
  PUBMED    16611888
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2006 May . 80(9):4292-303."
COMMENT     Sequence Label: pLB(N)CX-FLAG-CH3
FEATURES             Location/Qualifiers
     source          1..6907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             699..722
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             1419..2012
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2115..2137)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2223..2363
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2278..2297
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2378..2395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2549..3137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2629..2648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3308..4099,4100..4168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3931..3950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4169..4273)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4341..4359
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4931..5106
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5169..5368
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5569..5985
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5915..5937
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5953..5969
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6028..6426
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccccaag cttggatcct gaaccatgga ctacaaggac gacgatgaca
      721 agtcggccgc tggaggaggc cttggcttag atgatgagag caacaaccag caggctgcag
      781 ccacccagag cccaggcgat tctcgccgcc tgagtatcca gcgctgcatc cagtctctgg
      841 tccatgcttg ccagtgtcgg aatgccaatt gctcactgcc atcctgccag aagatgaagc
      901 gggttgtgca gcataccaag ggttgcaaac ggaaaaccaa tggcgggtgc cccatctgca
      961 agcagctcat tgccctctgc tgctaccatg ccaagcactg ccaggagaac aaatgcccgg
     1021 tgccgttctg cctaaacatc aagcagaagc tccggcagca acagctgcag caccgactac
     1081 agcaggccca aatgcttcgc aggaggatgg ccagcatgca gcggactggt gtggttgggc
     1141 agcaacaggg cctcccttcc cccactcctg ccactccaac gacaccaact ggccaacagc
     1201 caaccacccc gcagacgccc cagcccactt ctcagcctca gcctacccct cccaatagca
     1261 tgccacccta cttgcccagg actcaagctg ctggccctgt gtcccagggt aaggcagcag
     1321 gccaggtgac ccctccaacc cctcctcaga cttaggaatt caagcttgtt aacatcgata
     1381 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     1441 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     1501 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     1561 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     1621 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     1681 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     1741 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     1801 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     1861 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     1921 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     1981 gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc
     2041 ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt
     2101 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2161 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2221 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     2281 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     2341 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2401 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2461 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2521 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2581 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2641 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2701 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2761 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2821 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2881 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2941 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3001 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3061 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3121 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3181 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3241 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3301 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3361 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3421 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3481 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3541 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3601 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3661 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3721 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3781 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3841 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3901 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     3961 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4021 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4081 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4141 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4201 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4261 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4321 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattca
     4381 taccagatca ccgaaaactg tcctccaaat gtgtccccct cacactccca aattcgcggg
     4441 cttctgcctc ttagaccact ctaccctatt ccccacactc accggagcca aagccgcggc
     4501 ccttccgttt ctttgctttt gaaagacccc acccgtaggt ggcaagctag cttaagtaac
     4561 gccactttgc aaggcatgga aaaatacata actgagaata gaaaagttca gatcaaggtc
     4621 aggaacaaag aaacagctga ataccaaaca ggatatctgt ggtaagcggt tcctgccccg
     4681 gctcagggcc aagaacagat gagacagctg agtgatgggc caaacaggat atctgtggta
     4741 agcagttcct gccccggctc ggggccaaga acagatggtc cccagatgcg gtccagccct
     4801 cagcagtttc tagtgaatca tcagatgttt ccagggtgcc ccaaggacct gaaaatgacc
     4861 ctgtacctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttccgc
     4921 tctccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ttccgataga
     4981 ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca tccgaatcgt
     5041 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccacg acgggggtct
     5101 ttcatttggg ggctcgtccg ggatttggag acccctgccc agggaccacc gacccaccac
     5161 cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgttt
     5221 gatgttatgc gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt
     5281 ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt
     5341 gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca
     5401 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
     5461 ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt
     5521 ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt
     5581 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
     5641 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
     5701 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
     5761 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
     5821 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
     5881 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
     5941 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat tccgatctga
     6001 tcaagagaca ggatggggtc gtttcgcatg gccaagcctt tgtctcaaga agaatccacc
     6061 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     6121 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     6181 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     6241 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     6301 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     6361 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     6421 ggctaagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6481 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6541 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg
     6601 ggctcgatcc cctcgcgagt tggttcagct gctgcctgag gctggacgac ctcgcggagt
     6661 tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag cagcggctat ccgcgcatcc
     6721 atgcccccga actgcaggag tggggaggca cgatggccgc tttggtcgag gcggatccgg
     6781 ccattagcca tattattcat tggttatata gcataaatca atattggcta ttggccattg
     6841 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg
     6901 ccatgtt
//
