LOCUS       sequence-27611-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  TITLE     David Ron plasmids
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBABE
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..64)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        49..378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      229..364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      406..415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(457..478)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtggtggtac gtaggaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta
       61 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      121 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
      181 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
      241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
      301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
      361 ggcctaggct tttgcaaaaa gctggccgct ctagcgctac cggtcgccac catggtgagc
      421 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      481 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      541 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      601 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      661 ttcttcaagt ccgccatgcc cganngctac gtccagganc gcaccatctt cttcnnggac
      721 gacggcanct acaagacccg cgccgangtg aagttcgagg gcgacnncct gg
//
