LOCUS       sequence-276131-       14306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep97 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 14306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-Cep97
FEATURES             Location/Qualifiers
     source          1..14306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2404..4326)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGXGGGAGAGGGAGGXGXGGGAGGAGGAGAGGGAGAGGAGAGGXGAGGAGAGGG
                     AGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGGGAGGAGGAGAGGGAGGAGAGG
                     GAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGG
                     SGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPP
                     GRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRR
                     KKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLY
                     NLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKD
                     AIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEG
                     EEGQE"
     primer_bind     complement(4761..4779)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4847..4951
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4952..5020,5021..5812)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5170..5189)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5983..6571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6472..6491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6760..6780)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6778..7075
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7152..7751
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7152..7171)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7648..7668
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7842..7976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8114..8133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8410..8671
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8788..9360
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9368..9387
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9415..9434
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9415..9433
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9444..10064
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9517..9536)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10106..10227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10282..10661
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10662..10865
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10816..10836
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10867..10885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10888..10906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10916..10940
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11040..11063
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11064..11081
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11631..11654
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11667..11691
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(14296..14306,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3541 tgctcccccc ccycctgccc ctcctgctcc ygcccctcct gcccctcctg ctcctgcccc
     3601 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     3661 ccctcctcct gctcctgccc ctcctgctcc trcccctcct gctcctgccc ctcctgctcc
     3721 tgcccctcct cctgctcctg cccctcctgc ycctcctgcc cctcctccts ctcctscccc
     3781 tcctgcccct cctcctgctc ctgccccycc tcctsctcct gcccctcctg cccctcctgc
     3841 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     3901 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     3961 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     4021 ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt
     4081 gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc
     4141 tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg
     4201 tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg
     4261 tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc
     4321 agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat
     4381 acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt
     4441 cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat
     4501 caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag
     4561 cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt
     4621 ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg
     4681 aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga
     4741 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4801 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4861 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4921 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4981 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5041 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5101 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5161 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     5221 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5281 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5341 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5401 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5461 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     5521 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5581 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5641 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5701 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5761 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5821 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5881 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5941 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6001 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6061 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6121 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6181 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6241 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6301 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6361 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6421 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6481 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6541 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6601 cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     6661 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     6721 ggccatccag cctcgcgtcg aactagatga tccggctgtg gaatgtgtgt cagttagggt
     6781 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6841 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6901 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7021 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggagggt
     7081 gaccgccacg accggtgccg ccaccatccc ctgacccacg cccctgaccc ctcacaagga
     7141 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     7201 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     7261 tcgaccccga ccgccacatc gaacgcgtca ccgagctgca agaactcttc ctcacgcgcg
     7321 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     7381 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7441 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7501 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7561 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7621 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7681 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7741 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccg
     7801 gtccgacgcg gcccgacggg tccgaggggg gtcgacctcg aaacttgttt attgcagctt
     7861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7921 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     7981 ccgaacccct tcctcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     8041 cgttgttgcc attgctcgac caattctcat gtttgacagc ttatcatcgc agatccgtat
     8101 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8161 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8221 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8281 atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg
     8341 ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag
     8401 ggagggggaa atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     8461 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     8521 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     8581 cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc
     8641 catttgacca ttcaccacat tggtgtgcac ctccaagctg gccggggatc ctctagggcc
     8701 tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag
     8761 ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
     8821 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
     8881 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
     8941 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
     9001 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
     9061 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
     9121 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
     9181 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc
     9241 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
     9301 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag
     9361 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac
     9421 gactcactat agggcgaatt gatatgtcta gattagataa aagtaaagtg attaacagcg
     9481 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
     9541 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
     9601 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
     9661 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
     9721 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
     9781 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
     9841 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
     9901 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     9961 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
    10021 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac agcggatccc
    10081 gggaattcag atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata
    10141 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10201 tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg aatccatggc gcagaactgg
    10261 taggtatgga agatccgcgt tgacattgat tattgactag ttattaatag taatcaatta
    10321 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
    10381 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
    10441 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
    10501 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
    10561 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
    10621 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
    10681 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
    10741 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
    10801 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
    10861 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
    10921 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
    10981 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt accaccatgg
    11041 attataaaga cgatgatgac aagcaccacc accaccacca cgacaaggat tgtgaaatga
    11101 aacgcaccac actggacagc cctttgggga agctggagct gtctggttgt gagcagggtc
    11161 tgcacgaaat aatttttctg ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag
    11221 cccccgctgc ggttctcgga ggtccggagc ccctgatgca ggctacagcc tggctgaatg
    11281 cctatttcca ccagcccgag gctatcgaag agttccccgt gccggctctt caccatcccg
    11341 ttttccagca agagtcgttc accagacagg tgttatggaa gctgctgaag gttgtgaaat
    11401 tcggagaagt gatttcttac tctcacttag cagccctggc aggcaacccc gcagccacgg
    11461 cagcagtgaa gacggcactg agtggcaatc ctgtccctat cctgatcccg tgccacagag
    11521 tggtccaggg ggatttggat gtgggcggtt acgagggtgg actggccgtg aaggaatggc
    11581 ttctggccca tgaaggccac cggttgggga agccaggctt gggattcgaa ctggaggtgc
    11641 tgttccaggg ccccggtacc gccatcacaa gtttgtacaa aaaagcaggc ttaatggcgg
    11701 tggcgcgcgt ggacgcggct ttgcctcccg gagaaggatc agtggtcaat tggtcaggac
    11761 agggactaca gaaattaggt ccaaatttac cctgtgaagc tgatattcac actttgattc
    11821 tggataaaaa tcagattatt aaattggaaa atctggagaa atgcaaacga ttaatacagt
    11881 tatcagtagc taataatcgg ctggttcgga tgatgggtgt ggccaagctg acgttgcttc
    11941 gtgtattaaa tttgcctcat aatagcattg gctgtgtgga agggctaaag gaactagtac
    12001 atctggaatg gctgaatttg gcaggaaata atcttaaggc catggaacag atcaatagct
    12061 gcacagctct acagcatctc gatttatcag acaataatat atcccagata ggtgatctat
    12121 ctaaattggt atccctgaaa accctgcttt tacatggaaa catcatcacc tctcttagaa
    12181 tggcacctgc ttacctaccc agaagtcttg ctatactttc tttggcagaa aatgaaatcc
    12241 gagacttaaa tgagatctct tttttggcat ccttaactga attggaacag ttgtcgatta
    12301 tgaacaatcc ttgtgtgatg gcaacaccat ccatcccagg atttgactat cggccgtaca
    12361 tcgtcagctg gtgcctaaac ctcagagtcc tagatggata tgtgatttct cagaaggaaa
    12421 gtttgaaagc tgaatggctc tatagtcaag gcaaggggag agcatatcgg cctggccagc
    12481 acatccagct tgtccaatat ctggctacag tctgccccct cacttctaca ctaggtcttc
    12541 aaactgcaga ggatgccaaa ctagagaaga ttttgagcaa acagaggttt caccagaggc
    12601 agttgatgaa ccaaagccaa aatgaagagt tgtctcctct tgttcctgtt gaaacaaggg
    12661 catcccttat tcctgagcat tcaagccctg ttcaagattg ccagatatcc caggaaagtg
    12721 aacccgtcat tcaagtgaat tcttgggttg ggataaacag taatgatgat cagttatttg
    12781 cggttaagaa taattttcca gcctctgtac acactacgag atattctcga aatgatctgc
    12841 acctggaaga catacagacg gatgaggaca agttaaactg tagtcttctc tcttcagagt
    12901 ctacttttat gccagttgca tcaggactgt ctccactatc acctacagtt gagctgaggc
    12961 tgcagggcat taacttgggc ctagaagatg atggtgttgc agatgaatct gtgaaagggc
    13021 tggaaagcca ggtgttggat aaggaagagg aacagccttt atgggctgca aatgagaatt
    13081 ctgttcaaat gatgagaagt gaaatcaata cagaggtaaa tgagaaagct ggactattac
    13141 cttgtcctga gccaacaata atcagtgcta tcttgaagga tgataaccac agtcttacat
    13201 tttttcctga gtcaactgag cagaaacaat cagacataaa gaaaccagaa aatacacaac
    13261 cagaaaataa agaaaccata tctcaagcaa cttcagagaa acttcccatg attttaaccc
    13321 agagatctgt tgctttggga caagacaaag ttgcccttca gaaattaaat gatgcagcca
    13381 ccaagcttca ggcctgttgg cggggatttt atgccaggaa ctacaaccct caagccaaag
    13441 atgtgcgtta cgaaatccgg ctacgcagaa tgcaagagca cattgtctgc ttaactgatg
    13501 aaataaggag attacgaaaa gaaagagatg aagaacgtat taaaaaattt gtacaagaag
    13561 aagctttcag attcctttgg aaccaggtaa ggtctctaca ggtttggcaa cagacagtgg
    13621 accaacgtct aagttcctgg catactgatg ttcctcctat atcaagtact cttgtgccat
    13681 cgaaacatcc attatttacc caaagccagg agtcctcttg tgatcaaaat gctgattggt
    13741 ttattgcttc tgatgtagct cctcaagaga aatcattacc agaatttcca gactctggtt
    13801 ttcattcctc tctaacagaa caagttcatt cattgcagca ttctttggat tttgagaaaa
    13861 gttccacaga aggcagtgaa agctccataa tggggaattc cattgacaca gtcagatatg
    13921 gcaaagaatc agatttaggg gatgttagtg aagaacatgg tgaatggaat aaggaaagct
    13981 caaataacga gcaggacaat agtctgcttg aacagtattt aacttcagtt caacagctgg
    14041 aagatgctga tgagaggacc aattttgata cagagacaag agatagcaaa cttcacattg
    14101 cttgtttccc agtacagtta gatacattgt ctgacggtgc ttctgtagat gagagtcatg
    14161 gcatatctcc tcctttgcaa ggtgaaatta gccagacaca agagaattct aaattaaatg
    14221 cagaagttca agggcagcag ccagaatgtg attctacatt tcagctattg catgttggtg
    14281 ttactgtgta atgataccca gctttc
//
