LOCUS       sequence-276163-       12214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Centrin3 
            N-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-Centrin3
FEATURES             Location/Qualifiers
     source          1..12214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4763..4781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4849..4953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4954..5022,5023..5814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5172..5191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5985..6573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6474..6493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6762..6782)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6780..7077
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7154..7753
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7154..7173)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7650..7670
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7844..7978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8116..8135)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8412..8673
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8790..9362
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9370..9389
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9417..9436
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9417..9435
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9446..10066
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9519..9538)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10108..10229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10284..10663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10664..10867
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10818..10838
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10869..10887
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10890..10908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10918..10942
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11042..11065
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11066..11083
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11633..11656
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11669..11693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(12204..12214,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tgcccctcct scccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3541 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     3601 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     3661 ccctcctcct gctcckgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     3721 tcctgcccct cctccygctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     3781 ccctcctgcc cctcctcctc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     3841 scccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     3901 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     3961 cctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     4021 gcccctcctg ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct
     4081 ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc
     4141 cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc
     4201 cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct
     4261 ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg
     4321 tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga
     4381 atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg
     4441 gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc
     4501 atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg
     4561 agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga
     4621 ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac
     4681 ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat
     4741 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4801 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4861 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4921 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4981 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5041 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5101 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5161 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     5221 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5281 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5341 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5401 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5461 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     5521 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5581 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5641 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5701 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5761 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5821 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5881 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6001 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6061 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6121 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6181 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6241 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6361 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6421 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6481 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6541 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6601 ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     6661 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     6721 aaggccatcc agcctcgcgt cgaactagat gatccggctg tggaatgtgt gtcagttagg
     6781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6841 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6901 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6961 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7021 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7081 gtgaccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac ccctcacaag
     7141 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     7201 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7261 cgtcgacccc gaccgccaca tcgaacgcgt caccgagctg caagaactct tcctcacgcg
     7321 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     7381 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     7441 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     7501 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     7561 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     7621 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     7681 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7741 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     7801 cggtccgacg cggcccgacg ggtccgaggg gggtcgacct cgaaacttgt ttattgcagc
     7861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     7981 atccgaaccc cttcctcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
     8041 aacgttgttg ccattgctcg accaattctc atgtttgaca gcttatcatc gcagatccgt
     8101 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8161 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     8221 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     8281 cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc
     8341 ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc gcttttgcat
     8401 agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     8461 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     8521 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     8581 accactgaat tccgcattgc agagatattg tatttaagtg cctagctcga tacaataaac
     8641 gccatttgac cattcaccac attggtgtgc acctccaagc tggccgggga tcctctaggg
     8701 cctctgagct attccagaag tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa
     8761 agctccggat cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct
     8821 ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta
     8881 gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact
     8941 ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt
     9001 cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag
     9061 attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta
     9121 cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc
     9181 atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca
     9241 tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat
     9301 actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac
     9361 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgtaat
     9421 acgactcact atagggcgaa ttgatatgtc tagattagat aaaagtaaag tgattaacag
     9481 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca
     9541 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct
     9601 cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg
     9661 ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag
     9721 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac
     9781 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata
     9841 tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca
     9901 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca
     9961 agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt
    10021 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt acagcggatc
    10081 ccgggaattc agatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa
    10141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10201 ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgaatccatg gcgcagaact
    10261 ggtaggtatg gaagatccgc gttgacattg attattgact agttattaat agtaatcaat
    10321 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    10381 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    10441 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    10501 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    10561 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    10621 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    10681 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    10741 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    10801 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    10861 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
    10921 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
    10981 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttaccaccat
    11041 ggattataaa gacgatgatg acaagcacca ccaccaccac cacgacaagg attgtgaaat
    11101 gaaacgcacc acactggaca gccctttggg gaagctggag ctgtctggtt gtgagcaggg
    11161 tctgcacgaa ataatttttc tgggcaaggg gacgtctgca gctgatgccg tggaggtccc
    11221 agcccccgct gcggttctcg gaggtccgga gcccctgatg caggctacag cctggctgaa
    11281 tgcctatttc caccagcccg aggctatcga agagttcccc gtgccggctc ttcaccatcc
    11341 cgttttccag caagagtcgt tcaccagaca ggtgttatgg aagctgctga aggttgtgaa
    11401 attcggagaa gtgatttctt actctcactt agcagccctg gcaggcaacc ccgcagccac
    11461 ggcagcagtg aagacggcac tgagtggcaa tcctgtccct atcctgatcc cgtgccacag
    11521 agtggtccag ggggatttgg atgtgggcgg ttacgagggt ggactggccg tgaaggaatg
    11581 gcttctggcc catgaaggcc accggttggg gaagccaggc ttgggattcg aactggaggt
    11641 gctgttccag ggccccggta ccgccatcac aagtttgtac aaaaaagcag gcttaatgag
    11701 tttagctctg agaagtgagc ttctagtgga caaaacaaag aggaaaaaaa gaagagaact
    11761 gtctgaggaa cagaaacaag aaattaaaga tgcttttgaa ctatttgata cagacaaaga
    11821 tgaagcaata gattatcatg aattaaaggt ggcaatgaga gccttggggt ttgatgtaaa
    11881 aaaagctgat gtactgaaga ttcttaaaga ttatgacaga gaagccacag ggaaaatcac
    11941 ctttgaagat tttaatgaag ttgtgacaga ctggatattg gaaagagatc cccatgaaga
    12001 aatactcaag gcatttaaac tatttgatga tgatgattca ggtaaaataa gcttgaggaa
    12061 tttgcgacgt gttgctagag aattgggtga aaacatgagt gatgaagaac ttcgagctat
    12121 gatagaagaa tttgacaaag atggtgatgg agaaataaac caagaggagt tcattgctat
    12181 tatgactggt gacatttaat gatacccagc tttc
//
