LOCUS       sequence-276165-       13183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep63 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-Cep63
FEATURES             Location/Qualifiers
     source          1..13183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4751..4769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4837..4941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4942..5010,5011..5802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5160..5179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5973..6561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6462..6481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6750..6770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6768..7065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7142..7741
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7142..7161)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7638..7658
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7832..7966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8104..8123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8400..8661
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8778..9350
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9358..9377
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9405..9424
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9405..9423
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9434..10054
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9507..9526)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10096..10217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10272..10651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10652..10855
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10806..10826
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10857..10875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10878..10896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10906..10930
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11030..11053
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11054..11071
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11621..11644
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11657..11681
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             11899..11907
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(join(13173..13183,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tgcccctcct scccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3541 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     3601 tcctgctccc ccccccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     3661 cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     3721 cctgctcctg cccctcctgc ccctcctgcc cctcctccts ctcctscccc tcctgcccct
     3781 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
     3841 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     3901 gcccctcctg cccctcctcc tgctcctgcc cctcctccts ctcctgcccc tcctgcccct
     3961 ccygcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
     4021 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
     4081 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
     4141 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
     4201 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
     4261 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
     4321 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
     4381 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
     4441 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
     4501 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
     4561 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
     4621 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
     4681 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
     4741 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4801 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4921 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4981 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5041 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5101 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5161 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     5221 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5281 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5341 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5401 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5461 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     5521 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5581 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5641 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5701 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5761 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5821 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5881 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5941 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6001 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6061 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6121 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6181 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6241 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6301 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6361 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6421 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6481 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6541 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6601 tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     6661 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     6721 cctcgcgtcg aactagatga tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6781 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6841 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6901 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7021 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggagggt gaccgccacg
     7081 accggtgccg ccaccatccc ctgacccacg cccctgaccc ctcacaagga gacgaccttc
     7141 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     7201 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaccccga
     7261 ccgccacatc gaacgcgtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     7321 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     7381 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7441 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7501 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7561 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7621 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7681 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7741 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccg gtccgacgcg
     7801 gcccgacggg tccgaggggg gtcgacctcg aaacttgttt attgcagctt ataatggtta
     7861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat ccgaacccct
     7981 tcctcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc
     8041 attgctcgac caattctcat gtttgacagc ttatcatcgc agatccgtat ggtgcactct
     8101 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8161 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8221 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8281 cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt
     8341 gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa
     8401 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     8461 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8521 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc
     8581 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca
     8641 ttcaccacat tggtgtgcac ctccaagctg gccggggatc ctctagggcc tctgagctat
     8701 tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag ctccggatcg
     8761 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
     8821 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
     8881 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
     8941 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
     9001 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
     9061 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
     9121 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
     9181 gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt
     9241 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
     9301 aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     9361 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat
     9421 agggcgaatt gatatgtcta gattagataa aagtaaagtg attaacagcg cattagagct
     9481 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     9541 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     9601 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     9661 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     9721 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     9781 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     9841 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
     9901 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
     9961 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
    10021 attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac agcggatccc gggaattcag
    10081 atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10201 ctcatcaatg tatcttatca tgtctggtcg aatccatggc gcagaactgg taggtatgga
    10261 agatccgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    10321 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    10381 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    10441 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    10501 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    10561 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    10621 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
    10681 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
    10741 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
    10801 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
    10861 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
    10921 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
    10981 ccgatccagc ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga
    11041 cgatgatgac aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac
    11101 actggacagc cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat
    11161 aatttttctg ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc
    11221 ggttctcgga ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca
    11281 ccagcccgag gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca
    11341 agagtcgttc accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt
    11401 gatttcttac tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa
    11461 gacggcactg agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg
    11521 ggatttggat gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca
    11581 tgaaggccac cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg
    11641 ccccggtacc gccatcacaa gtttgtacaa aaaagcaggc accatggagg ctttgttaga
    11701 aggaatacaa aatcgagggc atggtggggg atttttgaca tcttgtgaag cagaactaca
    11761 ggagctcatg aaacagattg acataatggt ggctcataaa aaatctgaat gggaaggacg
    11821 tacacatgct ctagaaactt gcttgaaaat ccgtgaacag gaacttaaga gtcttaggag
    11881 tcagttggat gtgacacata aggaggttgg aatgttgcat cagcaggtag aagaacatga
    11941 aaaaatcaag caagagatga ccatggaata taagcaggag ttgaagaaac tacatgaaga
    12001 attatgcata ctgaagagaa gctatgaaaa gcttcagaaa aagcaaatga gggaattcag
    12061 aggaaatacc aaaaatcaca gggaagatcg gtctgaaatt gagaggttaa ctgcaaaaat
    12121 agaggaattc cgtcagaaat cgctggactg ggagaagcaa cgcttgattt atcagcaaca
    12181 ggtatcttca ctggaggcac aaaggaaggc tctggctgaa caatcagaga taattcaggc
    12241 tcagcttgtc aatcggaaac agaaattaga gtctgtggaa ctttctagcc aatcagaaat
    12301 tcaacactta agcagtaaac tggagcgggc taatgacact atctgtgcca atgagttgga
    12361 aatagagcgc ctcaccatga gggtcaatga cttggttgga accagtatga ctgtcctaca
    12421 ggagcagcag caaaaagaag aaaaattgag ggaatctgaa aaactattag aggctctgca
    12481 ggaagaaaag agagaattga aggcagctct tcagtctcaa gaaaatctca tacatgaggc
    12541 cagaatacaa aaggagaagt tacaagaaaa agtaaaggca actaacactc aacatgctgt
    12601 agaagctata agtttggaat ctgtgagtgc aacgtgtaaa cagctgagcc aagaactaat
    12661 ggaaaaatat gaagaactga agaggatgga agcacataac aatgaataca aagcagagat
    12721 taagaagttg aaagaacaga ttttacaggg tgaacaaagt tacagttctg cactagaagg
    12781 aatgaagatg gaaatctccc atctaactca ggagttacat cagcgagata tcactattgc
    12841 ttccaccaaa ggttcttcct cagacatgga aaagcgactc agagcagaga tgcaaaaggc
    12901 agaagacaaa gcagtagagc ataaggagat tttggatcag ctggagtcac tcaaattaga
    12961 aaatcgtcat ctttctgaaa tggtgatgaa attggaattg ggtttacatg agtgttcctt
    13021 gcctgtatct ccccttggtt caatagctac cagatttttg gaagaggagg aactgaggtc
    13081 tcatcacatt ctagagcgct tggatgccca tattgaagaa ctaaaaagag agagtgaaaa
    13141 gacagtgaga caattcacag ccttaaagta gaacccagct ttc
//
