LOCUS       sequence-27619-e         414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Harding HP, Zhang Y, Ron D
  TITLE     Protein translation and folding are coupled by an 
            endoplasmic-reticulum-resident kinase.
  JOURNAL   Nature. 1999 Jan 21. 397(6716):271-4.
  PUBMED    9930704
REFERENCE   2  (bases 1 to 414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1999 Jan 21. 397(6716):271-4."
COMMENT     Sequence Label: PERK 3: PERK.dC.9E10.pCDNA
FEATURES             Location/Qualifiers
     source          1..414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             173..202
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(233..251)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(234..251)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          335..400
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gactgaaagt aaatacgact ccgtgagtgc cgatgtcagt gacaacagct ggaatgacat
       61 gaagtactca ggatacggga aaacggttct gagacagcgg tcccgctcca tgagttcatc
      121 tggaacaaaa cattccagac agcccagctg ctcgtacacg ccactgcctg gcgagcagaa
      181 gctgatctct gaggaggatc tgtgactcga gcatgcatct agagggccct attctatagt
      241 gtcacctaaa tgctagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      301 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      361 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atgg
//
