LOCUS       sequence-276210-        5218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMP71 expression vector containing Kaede.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Bresser K, Dijkgraaf FE, Pritchard CEJ, Huijbers IJ, Song JY, Rohr 
            JC, Scheeren FA, Schumacher TN
  TITLE     A mouse model that is immunologically tolerant to reporter and 
            modifier proteins.
  JOURNAL   Commun Biol. 2020 May 29;3(1):273. doi: 10.1038/s42003-020-0979-0.
  PUBMED    32472011
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-29"; volume: "3(1):273. doi"; pages: " 
            10.1038/s42003-020-0979-0"
COMMENT     Sequence Label: pMP71-Kaede
FEATURES             Location/Qualifiers
     source          1..5218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1106..1783
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVVK
                     EGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIAT
                     NDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALLL
                     KGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLPD
                     NVK"
     LTR             1976..2566
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2591..2607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2591..2607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2604..2626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2615..2631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2639..2645,2646..2663,2664..2669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2684..2705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2822..2839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2993..3581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3073..3092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3752..4543,4544..4612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4375..4394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4613..4717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4785..4803
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4841..4863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4963..4982
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5176..5198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5190..5207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5191..5207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
       61 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
      121 aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat atgggccaaa
      181 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
      241 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
      301 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
      361 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
      421 cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt
      481 ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt gattgactgc
      541 ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc tgcccaggga
      601 ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc tgtctctgtc
      661 tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta gttggctaac
      721 tagatctgta tctggcggtc ccgcggaaga actgacgagt tcgtattccc ggccgcagcc
      781 cctgggagac gtcccagcgg cctcgggggc ccgttttgtg gcccattctg tatcagttaa
      841 cctacccgag tcggactttt tggagctccg ccactgtccg aggggtacgt ggctttgttg
      901 ggggacgaga gacagagaca cttcccgccc ccgtctgaat ttttgctttc ggttttacgc
      961 cgaaaccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact
     1021 gtgtttctgt atttgtctga aaattagctc gacaaagtta agtaatagtc cctctctcca
     1081 agctcactta caggcggccg ccaccatgag tctgattaaa ccagaaatga agatcaagct
     1141 gcttatggaa ggcaatgtaa acgggcacca gtttgttatt gagggagatg gaaaaggcca
     1201 tccttttgag ggaaaacaga gtatggacct tgtagtcaaa gaaggcgcac ctctcccttt
     1261 tgcctacgat atcttgacaa cagcattcca ttatggtaac agggtttttg ctaaataccc
     1321 agaccatata ccagactact tcaagcagtc gtttcccaaa gggttttctt gggagcgaag
     1381 cctgatgttc gaggacgggg gcgtttgcat cgctacaaat gacataacac tgaaaggaga
     1441 cacttttttt aacaaagttc gatttgatgg cgtaaacttt cccccaaatg gtcctgttat
     1501 gcagaagaag actctgaaat gggaggcatc cactgagaaa atgtatttgc gtgatggagt
     1561 gttgacgggc gatattacaa tggctctgct gcttaaagga gatgtccatt accgatgtga
     1621 cttcagaact acttacaaat ctaggcagga gggtgtcaag ttgccaggat atcactttgt
     1681 cgatcactgc atcagcatat tgaggcatga caaagactac aacgaggtta agctgtatga
     1741 gcacgctgtt gcccattctg gattgccgga caacgtcaag taaggatcca agcttaggcc
     1801 tgctcgcttt cttgctgtcc catttctatt aaaggttcct ttgttcccta agtccaacta
     1861 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctag cgctaagctt
     1921 aacacgagcc atagatagaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     1981 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     2041 aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa
     2101 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     2161 gttggaacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     2221 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac
     2281 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     2341 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     2401 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc
     2461 gtgttctcaa taaaccctct tgcagttgca tccgactcgt ggtctcgctg ttccttggga
     2521 gggtctcctc tgagtgattg actgcccacc tcgggggtct ttcattctcg agcagcttgg
     2581 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2641 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2701 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2761 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2881 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4741 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4801 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4861 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4921 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     4981 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     5041 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     5101 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     5161 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttagtact
//
