LOCUS       sequence-276219-        5221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMP71 expression vector containing AzamiGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Bresser K, Dijkgraaf FE, Pritchard CEJ, Huijbers IJ, Song JY, Rohr 
            JC, Scheeren FA, Schumacher TN
  TITLE     A mouse model that is immunologically tolerant to reporter and 
            modifier proteins.
  JOURNAL   Commun Biol. 2020 May 29;3(1):273. doi: 10.1038/s42003-020-0979-0.
  PUBMED    32472011
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-29"; volume: "3(1):273. doi"; pages: " 
            10.1038/s42003-020-0979-0"
COMMENT     Sequence Label: pMP71-AzamiGreen
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1100..1109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1106..1786
                     /codon_start=1
                     /product="Azami-Green fluorescent protein (Karasawa et al.,
                     2003)"
                     /label=Azami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGVNFPPNGPVMQKKTLKWEPSTEKMYVRDGVLKGDVNMALL
                     LEGGGHYRCDFKTTYKAKKDVRLPDYHFVDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     LTR             1979..2569
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2594..2610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2594..2610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2607..2629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2618..2634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2642..2648,2649..2666,2667..2672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2687..2708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2825..2842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2996..3584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3076..3095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3755..4546,4547..4615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4378..4397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4616..4720)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4788..4806
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4844..4866)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4966..4985
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5179..5201
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5193..5210
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5194..5210
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
       61 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
      121 aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat atgggccaaa
      181 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
      241 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
      301 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
      361 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
      421 cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt
      481 ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt gattgactgc
      541 ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc tgcccaggga
      601 ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc tgtctctgtc
      661 tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta gttggctaac
      721 tagatctgta tctggcggtc ccgcggaaga actgacgagt tcgtattccc ggccgcagcc
      781 cctgggagac gtcccagcgg cctcgggggc ccgttttgtg gcccattctg tatcagttaa
      841 cctacccgag tcggactttt tggagctccg ccactgtccg aggggtacgt ggctttgttg
      901 ggggacgaga gacagagaca cttcccgccc ccgtctgaat ttttgctttc ggttttacgc
      961 cgaaaccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact
     1021 gtgtttctgt atttgtctga aaattagctc gacaaagtta agtaatagtc cctctctcca
     1081 agctcactta caggcggccg ccaccatggt gagtgtgatt aaaccagaga tgaagatcaa
     1141 gctgtgtatg agaggcactg taaacgggca taatttcgtg attgaaggag aaggaaaagg
     1201 aaatccttac gagggaacgc agattttaga cctgaacgtc actgaaggcg cacctctgcc
     1261 tttcgcttac gatatcttga caacagtgtt ccagtacggc aacagggcat tcaccaagta
     1321 cccagcagat attcaggact atttcaagca gacttttcct gaggggtatc actgggaaag
     1381 aagcatgact tatgaagacc agggcatttg caccgccaca agcaacataa gcatgagggg
     1441 cgactgtttt ttctatgaca ttcgttttga tggtgtgaac tttcctccca atggtccggt
     1501 tatgcagaag aagactctta aatgggagcc atccactgag aaaatgtacg tacgtgatgg
     1561 agtgctgaag ggtgatgtta acatggctct gttgcttgaa ggaggtggcc attatcgatg
     1621 tgatttcaaa actacttaca aagcaaagaa ggatgtccgt ttgccagact atcactttgt
     1681 ggaccaccgc attgagattt tgaagcatga caaagattac aacaaggtca agctctatga
     1741 gaatgccgtt gctcgctatt ctatgctgcc gagtcaggcc aagtaaggat ccaagcttag
     1801 gcctgctcgc tttcttgctg tcccatttct attaaaggtt cctttgttcc ctaagtccaa
     1861 ctactaaact gggggatatt atgaagggcc ttgagcatct ggattctgcc tagcgctaag
     1921 cttaacacga gccatagata gaataaaaga ttttatttag tctccagaaa aaggggggaa
     1981 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     2041 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
     2101 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     2161 acagttggaa cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2221 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     2281 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2341 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     2401 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     2461 cccgtgttct caataaaccc tcttgcagtt gcatccgact cgtggtctcg ctgttccttg
     2521 ggagggtctc ctctgagtga ttgactgccc acctcggggg tctttcattc tcgagcagct
     2581 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2641 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2701 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2761 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3421 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3661 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3721 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3781 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3841 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3901 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3961 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4021 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4081 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4141 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4201 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4261 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4321 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4381 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4441 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4501 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4561 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4621 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4681 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4741 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4801 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4861 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4921 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4981 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5041 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5101 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     5161 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattagtac
     5221 t
//
