LOCUS       sequence-276229-       13091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            C-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-beta-tubulin_SNAP
FEATURES             Location/Qualifiers
     source          1..13091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2407..4335)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGXGGGA
                     GGAGGAGAGGGAGAGGGAGGAGXGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGGAGGXGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGR
                     GGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRP
                     PPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGG
                     RRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTS
                     LYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL
                     KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGD
                     EGEEGQE"
     primer_bind     complement(4770..4788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4856..4960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4961..5029,5030..5821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5179..5198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5992..6580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6481..6500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6769..6789)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6787..7084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7161..7760
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7161..7180)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7657..7677
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7851..7985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8123..8142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8419..8680
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8797..9369
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9377..9396
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9424..9443
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9424..9442
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9453..10073
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9526..9545)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10115..10236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10291..10670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10671..10874
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10825..10845
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10876..10894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10897..10915
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10925..10949
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11052..11076
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12418..12442)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12456..12479
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             13032..13049
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             13050..13073
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 srykrkrmag wssnwkrtra ymmstwatga ggcgcgcccg gccgcggggc agtgcatgta
       61 atcccttcag ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg
      121 ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca
      181 catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa
      241 cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca
      301 tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc
      361 ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc
      421 cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga
      481 ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt
      541 tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat
      601 ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct
      661 gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt
      721 cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga
      781 aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca
      841 ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg
      901 aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa
      961 gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat
     1021 atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg
     1081 ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg
     1141 gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa
     1201 agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca
     1261 cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc
     1321 cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata
     1381 cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa
     1441 attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg
     1501 tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg
     1561 atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     1621 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg
     1681 gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag
     1741 catatgctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     1801 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     1861 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     1921 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     1981 catatgctat cctcatgcat atacagtcag catatgatac ccagtagtag agtgggagtg
     2041 ctatcctttg catatgccgc cacctcccaa gggggcgtga attttcgctg cttgtccttt
     2101 tcctgctggt tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat
     2161 gtctgattgc tcaccaggta aatgtcgcta atgttttcca acgcgagaag gtgttgagcg
     2221 cggagctgag tgacgtgaca acatgggtat gcccaattgc cccatgttgg gaggacgaaa
     2281 atggtgacaa gacagatggc cagaaataca ccaacagcac gcatgatgtc tactggggat
     2341 ttattcttta gtgcggggga atacacggct tttaatacga ttgagggcgt ctcctaacaa
     2401 gttacatcac tcctgccctt cctcaccctc atctccatca cctccttcat ctccgtcatc
     2461 tccgtcatca ccctccgcgg cagccccttc caccataggt ggaaaccagg gaggcaaatc
     2521 tactccatcg tcaaagctgc acacagtcac cctgatattg caggtaggag cgggctttgt
     2581 cataacaagg tccttaatcg catccttcaa aacctcagca aatatatgag tttgtaaaaa
     2641 gaccatgaaa taacagacaa tggactccct tagcgggcca ggttgtgggc cgggtccagg
     2701 ggccattcca aaggggagac gactcaatgg tgtaagacga cattgtggaa tagcaagggc
     2761 agttcctcgc cttaggttgt aaagggaggt cttactacct ccatatacga acacaccggc
     2821 gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc ctagccagga gagctcttaa
     2881 accttctgca atgttctcaa atttcgggtt ggaacctcct tgaccacgat gctttccaaa
     2941 ccaccctcct tttttgcgcc tgcctccatc accctgaccc cggggtccag tgcttgggcc
     3001 ttctcctggg tcatctgcgg ggccctgctc tatcgctccc gggggcacgt caggctcacc
     3061 atctgggcca ccttcttggt ggtattcaaa ataatcggct tcccctacag ggtggaaaaa
     3121 tggccttcta cctggagggg gcctgcgcgg tggagacccg gatgatgatg actgactact
     3181 gggactcctg ggcctctttt ctccacgtcc acgacctctc cccctggctc tttcacgact
     3241 tccccccctg gctctttcac gtcctctacc ccggcggcct ccactacctc ctcgaccccg
     3301 gcctccacta cctcctcgac cccggcctcc actgcctcct cgaccccggc ctccacctcc
     3361 tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     3421 tcctgcccct cctgcccctc ctgctccwsc scctcctgcc cctcctcctg ctcctgcccc
     3481 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     3541 ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     3601 tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc
     3661 tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc
     3721 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc cycctcctkc
     3781 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     3841 ccctcctgcc cctcctccts ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     3901 tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
     3961 tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     4021 tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg
     4081 ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg
     4141 tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc
     4201 tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga
     4261 gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg
     4321 cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc
     4381 tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc
     4441 cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct
     4501 cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg
     4561 cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg
     4621 atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg
     4681 gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt
     4741 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4801 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4981 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5041 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5101 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5161 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     5221 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5281 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5341 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5401 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5461 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     5521 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5581 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5641 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5701 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5761 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5821 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5881 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5941 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6001 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6061 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6121 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6181 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6241 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6301 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6361 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6421 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6481 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6541 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6601 tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg
     6661 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     6721 aatggggaag gccatccagc ctcgcgtcga actagatgat ccggctgtgg aatgtgtgtc
     6781 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6841 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6901 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6961 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7021 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7081 ttggagggtg accgccacga ccggtgccgc caccatcccc tgacccacgc ccctgacccc
     7141 tcacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7201 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7261 gccacaccgt cgaccccgac cgccacatcg aacgcgtcac cgagctgcaa gaactcttcc
     7321 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7381 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7441 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7501 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     7561 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     7621 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     7681 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     7741 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     7801 cacgacccgg tccgacgcgg cccgacgggt ccgagggggg tcgacctcga aacttgttta
     7861 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7921 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7981 ggatctgatc cgaacccctt cctcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     8041 tccgggcaac gttgttgcca ttgctcgacc aattctcatg tttgacagct tatcatcgca
     8101 gatccgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8161 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     8221 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     8281 tgcttcgcga tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc
     8341 gaaaagcggg gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct
     8401 tttgcatagg gagggggaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     8461 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     8521 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     8581 tggacgaacc actgaattcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     8641 aataaacgcc atttgaccat tcaccacatt ggtgtgcacc tccaagctgg ccggggatcc
     8701 tctagggcct ctgagctatt ccagaagtag tgaagaggct tttttggagg cctaggcttt
     8761 tgcaaaaagc tccggatcga tcctgagaac ttcagggtga gtttggggac ccttgattgt
     8821 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
     8881 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
     8941 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
     9001 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
     9061 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
     9121 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
     9181 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc
     9241 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
     9301 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt
     9361 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     9421 ttgtaatacg actcactata gggcgaattg atatgtctag attagataaa agtaaagtga
     9481 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     9541 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     9601 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     9661 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     9721 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     9781 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
     9841 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
     9901 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     9961 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
    10021 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca
    10081 gcggatcccg ggaattcaga tcttattaaa gcagaacttg tttattgcag cttataatgg
    10141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggtcga atccatggcg
    10261 cagaactggt aggtatggaa gatccgcgtt gacattgatt attgactagt tattaatagt
    10321 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    10381 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    10441 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    10501 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    10561 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    10621 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    10681 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    10741 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    10801 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    10861 atataagcag agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc
    10921 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
    10981 tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttg
    11041 gtaccgccat cacaagtttg tacaaaaaag caggcttaac caccatgagg gaaatcgtgc
    11101 acatccaggc tggtcagtgt ggcaaccaga tcggtgccaa gttctgggag gtgatcagtg
    11161 atgaacatgg catcgacccc accggcacct accacgggga cagcgacctg cagctggacc
    11221 gcatctctgt gtactacaat gaagccacag gtggcaaata tgttcctcgt gccatcctgg
    11281 tggatctaga acctgggacc atggactctg ttcgctcagg tccttttggc cagatcttta
    11341 gaccagacaa ctttgtattt ggtcagtctg gggcaggtaa caactgggcc aaaggccact
    11401 acacagaggg cgccgagctg gttgattctg tcctggatgt ggtacggaag gaggcagaga
    11461 gctgtgactg cctgcagggc ttccagctga cccactcact gggcgggggc acaggctctg
    11521 gaatgggcac tctccttatc agcaagatcc gagaagaata ccctgatcgc atcatgaata
    11581 ccttcagtgt ggtgccttca cccaaagtgt ctgacaccgt ggtcgagccc tacaatgcca
    11641 ccctctccgt ccatcagttg gtagagaata ctgatgagac ctattgcatt gacaacgagg
    11701 ccctctatga tatctgcttc cgcactctga agctgaccac accaacctac ggggatctga
    11761 accaccttgt ctcagccacc atgagtggtg tcaccacctg cctccgtttc cctggccagc
    11821 tcaatgctga cctccgcaag ttggcagtca acatggtccc cttcccacgt ctccatttct
    11881 ttatgcctgg ctttgcccct ctcaccagcc gtggaagcca gcagtatcga gctctcacag
    11941 tgccggaact cacccagcag gtcttcgatg ccaagaacat gatggctgcc tgtgaccccc
    12001 gccacggccg atacctcacc gtggctgctg tcttccgtgg tcggatgtcc atgaaggagg
    12061 tcgatgagca gatgcttaac gtgcagaaca agaacagcag ctactttgtg gaatggatcc
    12121 ccaacaatgt caagacagcc gtctgtgaca tcccacctcg tggcctcaag atggcagtca
    12181 ccttcattgg caatagcaca gccatccagg agctcttcaa gcgcatctcg gagcagttca
    12241 ctgccatgtt ccgccggaag gccttcctcc actggtacac aggcgagggc atggacgaga
    12301 tggagttcac cgaggctgag agcaacatga acgacctcgt ctctgagtat cagcagtacc
    12361 aggatgccac cgcagaagag gaggaggatt tcggtgagga ggccgaagag gaggcctacc
    12421 cagctttctt gtacaaagtg gtgatggcag ctagcctgga ggtgctgttc cagggcccct
    12481 tcgaaatgga caaggattgt gaaatgaaac gcaccacact ggacagccct ttggggaagc
    12541 tggagctgtc tggttgtgag cagggtctgc acgaaataat ttttctgggc aaggggacgt
    12601 ctgcagctga tgccgtggag gtcccagccc ccgctgcggt tctcggaggt ccggagcccc
    12661 tgatgcaggc tacagcctgg ctgaatgcct atttccacca gcccgaggct atcgaagagt
    12721 tccccgtgcc ggctcttcac catcccgttt tccagcaaga gtcgttcacc agacaggtgt
    12781 tatggaagct gctgaaggtt gtgaaattcg gagaagtgat ttcttactct cacttagcag
    12841 ccctggcagg caaccccgca gccacggcag cagtgaagac ggcactgagt ggcaatcctg
    12901 tccctatcct gatcccgtgc cacagagtgg tccaggggga tttggatgtg ggcggttacg
    12961 agggtggact ggccgtgaag gaatggcttc tggcccatga aggccaccgg ttggggaagc
    13021 caggcttggg acaccaccac caccaccacg attataaaga cgatgatgac aagtaatgag
    13081 gcgcgcccsr c
//
