LOCUS       sequence-276289-       13071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            N-terminally fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-gamma-tubulin
FEATURES             Location/Qualifiers
     source          1..13071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2708..4636)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGXGGXGXGGGAGXGGGAGGXGXGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGXGGAGAXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGR
                     GGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRP
                     PPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGG
                     RRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTS
                     LYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL
                     KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGD
                     EGEEGQE"
     primer_bind     complement(5071..5089)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5157..5261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5262..5330,5331..6122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5480..5499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6293..6881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6782..6801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7070..7090)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7088..7385
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7407..7805
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7849..7983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8121..8140)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8417..8678
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8795..9367
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9375..9394
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9422..9441
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9422..9440
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9451..10071
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9524..9543)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10113..10234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10289..10668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10669..10872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10823..10843
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10874..10892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10895..10913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10923..10947
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11047..11070
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11071..11088
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11638..11661
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11674..11698
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13061..13071,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctnnngctc ctgcccctcc tscccctcct gctcctgccc
     3781 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     3841 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
     3901 ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc
     3961 ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc
     4021 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ycctcctgcc ccycctccts
     4081 ctcctscccc tcctgcccct cctcctscwc ctgcccctcc tcctsctcct scccctccts
     4141 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     4201 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     4261 ctcctgcccc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     4321 ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt
     4381 gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat
     4441 gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc
     4501 ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg
     4561 agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg
     4621 gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg
     4681 ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc
     4741 ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc
     4801 tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt
     4861 gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg
     4921 gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact
     4981 ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg
     5041 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5101 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     5521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     5821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6421 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6901 ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct
     6961 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     7021 taatggggaa ggccatccag cctcgcgtcg aactagatga tccggctgtg gaatgtgtgt
     7081 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7141 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7201 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7261 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7321 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7381 tttggagggt gaccgccacg accggtatgg ccaagccttt gtctcaagaa gaatccaccc
     7441 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     7501 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     7561 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     7621 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     7681 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     7741 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     7801 gctaacggtc cgacgcggcc cgacgggtcc gaggggggtc gacctcgaaa cttgtttatt
     7861 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7921 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7981 atctgatccg aaccccttcc tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     8041 cgggcaacgt tgttgccatt gctcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     8101 tccgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8161 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8221 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8281 cttcgcgatg tacgggccag atatacgcgt atctgagggg actagggtgt gtttaggcga
     8341 aaagcggggc ttcggttgta cgcggttagg agtcccctca ggatatagta gtttcgcttt
     8401 tgcataggga gggggaaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     8461 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     8521 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     8581 gacgaaccac tgaattccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa
     8641 taaacgccat ttgaccattc accacattgg tgtgcacctc caagctggcc ggggatcctc
     8701 tagggcctct gagctattcc agaagtagtg aagaggcttt tttggaggcc taggcttttg
     8761 caaaaagctc cggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     8821 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     8881 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     8941 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     9001 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     9061 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     9121 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     9181 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     9241 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     9301 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     9361 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     9421 gtaatacgac tcactatagg gcgaattgat atgtctagat tagataaaag taaagtgatt
     9481 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     9541 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     9601 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     9661 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     9721 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     9781 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     9841 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     9901 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     9961 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
    10021 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc
    10081 ggatcccggg aattcagatc ttattaaagc agaacttgtt tattgcagct tataatggtt
    10141 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10201 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggtcgaat ccatggcgca
    10261 gaactggtag gtatggaaga tccgcgttga cattgattat tgactagtta ttaatagtaa
    10321 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    10381 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    10441 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    10501 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    10561 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    10621 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    10681 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    10741 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    10801 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    10861 ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
    10921 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
    10981 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact taagcttacc
    11041 accatggatt ataaagacga tgatgacaag caccaccacc accaccacga caaggattgt
    11101 gaaatgaaac gcaccacact ggacagccct ttggggaagc tggagctgtc tggttgtgag
    11161 cagggtctgc acgaaataat ttttctgggc aaggggacgt ctgcagctga tgccgtggag
    11221 gtcccagccc ccgctgcggt tctcggaggt ccggagcccc tgatacaggc tacagcctgg
    11281 ctgaatgcct atttccacca gcccgaggct atcgaagagt tccccgtgcc ggctcttcac
    11341 catcccgttt tccagcaaga gtcgttcacc agacaggtgt tatggaagct gctgaaggtt
    11401 gtgaaattcg gagaagtgat ttctgagtca cacttagcag ccctggtagg caaccccgca
    11461 gccacggcag cagtgaatac ggcactggat ggcaatcctg tccctatcct gatcccgtgc
    11521 cacagagtgg tccaggggga ttcggatgtg gggccgtacc ttggtggact ggccgtgaag
    11581 gaatggcttc tggcccatga aggccaccgg ttggggaagc caggcttggg attcgaactg
    11641 gaggtgctgt tccagggccc cggtaccgcc atcacaagtt tgtacaaaaa agcaggctta
    11701 atgccgaggg aaatcatcac cctacagttg ggccagtgcg gcaatcagat tgggttcgag
    11761 ttctggaaac agctgtgcgc cgagcatggt atcagccccg agggcatcgt ggaggagttc
    11821 gccaccgagg gcactgaccg caaggacgtc tttttctacc aggcagacga tgagcactac
    11881 atcccccggg ccgtgctgct ggacttggaa ccccgggtga tccactccat cctcaactcc
    11941 ccctatgcca agctctacaa cccagagaac atctacctgt cggaacatgg aggaggagct
    12001 ggcaacaact gggccagcgg attctcccag ggagaaaaga tccatgagga catttttgac
    12061 atcatagacc gggaggcaga tggtagtgac agtctagagg gctttgtgct gtgtcactcc
    12121 attgctgggg ggacaggctc tggactgggt tcctacctct tagaacggct gaatgacagg
    12181 tatcctaaga agctggtgca gacatactca gtgtttccca accaggacga gatgagcgat
    12241 gtggtggtcc agccttacaa ttcactcctc acactcaaga ggctgacgca gaatgcagac
    12301 tgtgtggtgg tgctggacaa cacagccctg aaccggattg ccacagaccg cctgcacatc
    12361 cagaacccat ccttctccca gatcaaccag ctggtgtcta ccatcatgtc agccagcacc
    12421 accaccctgc gctaccctgg ctacatgaac aatgacctca tcggcctcat cgcctcgctc
    12481 attcccaccc cacggctcca cttcctcatg accggctaca cccctctcac tacggaccag
    12541 tcagtggcca gcgtgaggaa gaccacggtc ctggatgtca tgaggcggct gctgcagccc
    12601 aagaacgtga tggtgtccac aggccgagac cgccagacca accactgcta catcgccatc
    12661 ctcaacatca tccagggaga ggtggacccc acccaggtcc acaagagctt gcagaggatc
    12721 cgggaacgca agttggccaa cttcatcccg tggggccccg ccagcatcca ggtggccctg
    12781 tcgaggaagt ctccctacct gccctcggcc caccgggtca gcgggctcat gatggccaac
    12841 cacaccagca tctcctcgct cttcgagaga acctgtcgcc agtatgacaa gctgcgtaag
    12901 cgggaggcct tcctggagca gttccgcaag gaggacatgt tcaaggacaa ctttgatgag
    12961 atggacacat ccagggagat tgtgcagcag ctcatcgatg agtaccatgc ggccacacgg
    13021 ccagactaca tctcctgggg cacccaggag cagtaatgat acccagcttt c
//
