LOCUS       sequence-276323-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescently-tagged human Type I procollagen alpha-1 
            chain with eGFP replacing exon 2-3 retaining N-propeptide minor 
            triple helix and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  TITLE     Fluorescent protein-tagged human type I procollagen expression 
            vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: eGFP-hCOL1A1
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     843..862
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        843..861
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(870..903)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(1140..1142,1143..1850)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(1182..1203)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1443..1462)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(6254..6271)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6260..6484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6530..6958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6617..6636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6827..6848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6967..6987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6972..7301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7152..7287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7214..7233
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7368..8162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7422..7441)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8032..8051
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8336..8457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8373..8392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8427..8446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8506..8522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8506..8522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8519..8541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8530..8546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8554..8560,8561..8578,8579..8584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8599..8620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8737..8754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8908..9496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8988..9007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9667..10458,10459..10527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10290..10309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10528..10632)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagaattt
      841 tgtaatacga ctcactatag ggcggccgca taacttcgta tagcatacat tatacgaagt
      901 tatggatcag gccaaatcgg ccgagctcga attcgtcgag agcggagacg ggagtttctc
      961 ctcggggtcg gagcaggagg cacgcggagt gtgaggccac gcatgagcgg acgctaaccc
     1021 cctccccagc cacaaagagt ctacatgtct agggtctaga catgttcagc tttgtggacc
     1081 tccggctcct gctcctctta gcggccaccg ccctcctgac gcacggccaa gaggatatcg
     1141 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1201 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1261 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1321 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1381 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1441 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1501 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1561 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1621 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1681 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1741 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1801 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac gaccaagaaa
     1861 ccaccggcgt cgagggaccc aagggagaca ctggcccccg aggcccaagg ggacccgcag
     1921 gcccccctgg ccgagatggc atccctggac agcctggact tcccggaccc cccggacccc
     1981 ccggacctcc cggaccccct ggcctcggag gaaactttgc tccccagctg tcttatggct
     2041 atgatgagaa atcaaccgga ggaatttccg tgcctggccc catgggtccc tctggtcctc
     2101 gtggtctccc tggcccccct ggtgcacctg gtccccaagg cttccaaggt ccccctggtg
     2161 agcctggcga gcctggagct tcaggtccca tgggtccccg aggtccccca ggtccccctg
     2221 gaaagaatgg agatgatggg gaagctggaa aacctggtcg tcctggtgag cgtgggcctc
     2281 ctgggcctca gggtgctcga ggattgcccg gaacagctgg cctccctgga atgaagggac
     2341 acagaggttt cagtggtttg gatggtgcca agggagatgc tggtcctgct ggtcctaagg
     2401 gtgagcctgg cagccctggt gaaaatggag ctcctggtca gatgggcccc cgtggcctgc
     2461 ctggtgagag aggtcgccct ggagcccctg gccctgctgg tgctcgtgga aatgatggtg
     2521 ctactggtgc tgccgggccc cctggtccca ccggccccgc tggtcctcct ggcttccctg
     2581 gtgctgttgg tgctaagggt gaagctggtc cccaagggcc ccgaggctct gaaggtcccc
     2641 agggtgtgcg tggtgagcct ggcccccctg gccctgctgg tgctgctggc cctgctggaa
     2701 accctggtgc tgatggacag cctggtgcta aaggtgccaa tggtgctcct ggtattgctg
     2761 gtgctcctgg cttccctggt gcccgaggcc cctctggacc ccagggcccc ggcggccctc
     2821 ctggtcccaa gggtaacagc ggtgaacctg gtgctcctgg cagcaaagga gacactggtg
     2881 ctaagggaga gcctggccct gttggtgttc aaggaccccc tggccctgct ggagaggaag
     2941 gaaagcgagg agctcgaggt gaacccggac ccactggcct gcccggaccc cctggcgagc
     3001 gtggtggacc tggtagccgt ggtttccctg gcgcagatgg tgttgctggt cccaagggtc
     3061 ccgctggtga acgtggttct cctggccctg ctggccccaa aggatctcct ggtgaagctg
     3121 gtcgtcccgg tgaagctggt ctgcctggtg ccaagggtct gactggaagc cctggcagcc
     3181 ctggtcctga tggcaaaact ggcccccctg gtcccgccgg tcaagatggt cgccccggac
     3241 ccccaggccc acctggtgcc cgtggtcagg ctggtgtgat gggattccct ggacctaaag
     3301 gtgctgctgg agagcccggc aaggctggag agcgaggtgt tcccggaccc cctggcgctg
     3361 tcggtcctgc tggcaaagat ggagaggctg gagctcaggg accccctggc cctgctggtc
     3421 ccgctggcga gagaggtgaa caaggccctg ctggctcccc cggattccag ggtctccctg
     3481 gtcctgctgg tcctccaggt gaagcaggca aacctggtga acagggtgtt cctggagacc
     3541 ttggcgcccc tggcccctct ggagcaagag gcgagagagg tttccctggc gagcgtggtg
     3601 tgcaaggtcc ccctggtcct gctggtcccc gaggggccaa cggtgctccc ggcaacgatg
     3661 gtgctaaggg tgatgctggt gcccctggag ctcccggtag ccagggcgcc cctggccttc
     3721 agggaatgcc tggtgaacgt ggtgcagctg gtcttccagg gcctaagggt gacagaggtg
     3781 atgctggtcc caaaggtgct gatggctctc ctggcaaaga tggcgtccgt ggtctgaccg
     3841 gccccattgg tcctcctggc cctgctggtg cccctggtga caagggtgaa agtggtccca
     3901 gcggccctgc tggtcccact ggagctcgtg gtgcccccgg agaccgtggt gagcctggtc
     3961 cccccggccc tgctggcttt gctggccccc ctggtgctga cggccaacct ggtgctaaag
     4021 gcgaacctgg tgatgctggt gctaaaggcg atgctggtcc ccctggccct gccggacccg
     4081 ctggaccccc tggccccatt ggtaatgttg gtgctcctgg agccaaaggt gctcgcggca
     4141 gcgctggtcc ccctggtgct actggtttcc ctggtgctgc tggccgagtc ggtcctcctg
     4201 gcccctctgg aaatgctgga ccccctggcc ctcctggtcc tgctggcaaa gaaggcggca
     4261 aaggtccccg tggtgagact ggccctgctg gacgtcctgg tgaagttggt ccccctggtc
     4321 cccctggccc tgctggcgag aaaggatccc ctggtgctga tggtcctgct ggtgctcctg
     4381 gtactcccgg gcctcaaggt attgctggac agcgtggtgt ggtcggcctg cctggtcaga
     4441 gaggagagag aggcttccct ggtcttcctg gcccctctgg tgaacctggc aaacaaggtc
     4501 cctctggagc aagtggtgaa cgtggtcccc ctggtcccat gggcccccct ggattggctg
     4561 gaccccctgg tgaatctgga cgtgaggggg ctcctggtgc cgaaggttcc cctggacgag
     4621 acggttctcc tggcgccaag ggtgaccgtg gtgagaccgg ccccgctgga ccccctggtg
     4681 ctcctggtgc tcctggtgcc cctggccccg ttggccctgc tggcaagagt ggtgatcgtg
     4741 gtgagactgg tcctgctggt cccgccggtc ctgtcggccc tgttggcgcc cgtggccccg
     4801 ccggacccca aggcccacgt ggtgacaagg gtgagacagg cgaacagggc gacagaggca
     4861 taaagggtca ccgtggcttc tctggcctcc agggtccccc tggccctcct ggctctcctg
     4921 gtgaacaagg tccctctgga gcctctggtc ctgctggtcc ccgaggtccc cctggctctg
     4981 ctggtgctcc tggcaaagat ggactcaacg gtctccctgg ccccattggg ccccctggtc
     5041 ctcgcggtcg cactggtgat gctggtcctg ttggtccccc cggccctcct ggacctcctg
     5101 gtccccctgg tcctcccagc gctggtttcg acttcagctt cctgccccag ccacctcaag
     5161 agaaggctca cgatggtggc cgctactacc gggctgatga tgccaatgtg gttcgtgacc
     5221 gtgacctcga ggtggacacc accctcaaga gcctgagcca gcagatcgag aacatccgga
     5281 gcccagaggg cagccgcaag aaccccgccc gcacctgccg tgacctcaag atgtgccact
     5341 ctgactggaa gagtggagag tactggattg accccaacca aggctgcaac ctggatgcca
     5401 tcaaagtctt ctgcaacatg gagactggtg agacctgcgt gtaccccact cagcccagtg
     5461 tggcccagaa gaactggtac atcagcaaga accccaagga caagaggcat gtctggttcg
     5521 gcgagagcat gaccgatgga ttccagttcg agtatggcgg ccagggctcc gaccctgccg
     5581 atgtggccat ccagctgacc ttcctgcgcc tgatgtccac cgaggcctcc cagaacatca
     5641 cctaccactg caagaacagc gtggcctaca tggaccagca gactggcaac ctcaagaagg
     5701 ccctgctcct ccagggctcc aacgagatcg agatccgcgc cgagggcaac agccgcttca
     5761 cctacagcgt cactgtcgat ggctgcacga gtcacaccgg agcctggggc aagacagtga
     5821 ttgaatacaa aaccaccaag acctcccgcc tgcgcatcat cgatgtggcc cccttggacg
     5881 ttggtgcccc agaccaggaa ttcggcttcg acgttggcca tgtctgcttc ctgtaaactc
     5941 cctccatccc aacctggctc cctcccaccc aaccaacttt ccccccaacc cggaaacaga
     6001 caagcaaccc aaactgaacc ccctcaaaag ccaaaaaatg ggagacaatt tcacatggac
     6061 tttggaaaat atttttttcc tttgcattca tctctcaaac ttagttttta tctttgacca
     6121 accgaacatg accaaaaacc aaaagtgcat tcaaccttac caaaaaaaaa aaaaaaactc
     6181 tccagcgctg gatcccctgg tgctgatggt cctgctggtg ctcctggtac tccccctaaa
     6241 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6301 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6361 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6421 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6481 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     6541 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6601 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6661 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6721 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6781 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6841 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6901 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6961 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7021 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7081 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7141 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7201 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7261 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7321 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7381 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7441 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7501 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7561 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7621 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7681 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7741 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7801 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     7861 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     7921 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     7981 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8041 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8101 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8161 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     8221 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     8281 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     8341 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8401 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8461 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8581 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8641 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8701 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8761 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8821 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8881 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8941 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9001 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9061 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9121 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9181 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9241 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9301 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9361 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9421 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9481 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9541 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9601 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9661 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9721 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9781 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9841 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9901 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9961 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10021 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10081 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10141 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10201 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10261 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10321 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10381 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10441 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10501 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10561 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10621 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcg
//
