LOCUS       sequence-276339-       12523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep57 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 12523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-Cep57
FEATURES             Location/Qualifiers
     source          1..12523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5069..5087)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5155..5259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5260..5328,5329..6120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5478..5497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6291..6879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6780..6799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7068..7088)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7086..7383
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7405..7803
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7847..7981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8119..8138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8415..8676
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8793..9365
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9373..9392
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9420..9439
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9420..9438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9449..10069
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9522..9541)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10111..10232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10287..10666
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10667..10870
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10821..10841
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10872..10890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10893..10911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10921..10945
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11045..11068
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11069..11086
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11636..11659
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11672..11696
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      11697..11706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(join(12513..12523,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     3841 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     3901 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcnccc tcctscccct
     3961 cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
     4021 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     4081 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     4141 cctcctgccc ctcctccysc tcctccccct cctgcccctc ctcctgctcc tgcccctcct
     4201 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     4261 cctgcccctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     4321 gctcctgccc ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg
     4381 gtccctttgc agccaatgca acttggacgt ttttggggtc tccggacacc atctctatgt
     4441 cttggccctg atcctgagcc gcccggggct cctggtcttc cgcctcctcg tcctcgtcct
     4501 cttccccgtc ctcgtccatg gttatcaccc cctcttcttt gaggtccact gccgccggag
     4561 ccttctggtc cagatgtgtc tcccttctct cctaggccat ttccaggtcc tgtacctggc
     4621 ccctcgtcag acatgattca cactaaaaga gatcaataga catctttatt agacgacgct
     4681 cagtgaatac agggagtgca gactcctgcc ccctccaaca gcccccccac cctcatcccc
     4741 ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc ccatcctccg aaccatcctc
     4801 gtcctcatca ccaattactc gcagcccgga aaactcccgc tgaacatcct caagatttgc
     4861 gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc tttttgctgg acggtaggga
     4921 tggggattct cgggacccct cctcttcctc ttcaaggtca ccagacagag atgctactgg
     4981 ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag cttatcgatg ataagctgtc
     5041 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5101 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5221 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5281 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5341 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5401 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5461 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5521 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5581 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5641 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5701 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5761 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     5821 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5881 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5941 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6001 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6061 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6421 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6901 ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc atctacctgc
     6961 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
     7021 atggggaagg ccatccagcc tcgcgtcgaa ctagatgatc cggctgtgga atgtgtgtca
     7081 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7141 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7201 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7321 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7381 tggagggtga ccgccacgac cggtatggcc aagcctttgt ctcaagaaga atccaccctc
     7441 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     7501 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     7561 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     7621 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     7681 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     7741 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     7801 taacggtccg acgcggcccg acgggtccga ggggggtcga cctcgaaact tgtttattgc
     7861 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7921 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     7981 ctgatccgaa ccccttcctc gaccaattct catgtttgac agcttatcat cgcagatccg
     8041 ggcaacgttg ttgccattgc tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     8101 cgtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8161 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8221 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8281 tcgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa
     8341 agcggggctt cggttgtacg cggttaggag tcccctcagg atatagtagt ttcgcttttg
     8401 catagggagg gggaaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     8461 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     8521 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     8581 cgaaccactg aattccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata
     8641 aacgccattt gaccattcac cacattggtg tgcacctcca agctggccgg ggatcctcta
     8701 gggcctctga gctattccag aagtagtgaa gaggcttttt tggaggccta ggcttttgca
     8761 aaaagctccg gatcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt
     8821 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     8881 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     8941 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     9001 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     9061 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     9121 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     9181 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
     9241 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     9301 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
     9361 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt
     9421 aatacgactc actatagggc gaattgatat gtctagatta gataaaagta aagtgattaa
     9481 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     9541 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     9601 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     9661 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     9721 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     9781 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
     9841 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
     9901 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
     9961 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
    10021 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg
    10081 atcccgggaa ttcagatctt attaaagcag aacttgttta ttgcagctta taatggttac
    10141 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10201 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgaatcc atggcgcaga
    10261 actggtaggt atggaagatc cgcgttgaca ttgattattg actagttatt aatagtaatc
    10321 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    10381 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    10441 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    10501 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    10561 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    10621 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    10681 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    10741 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    10801 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    10861 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
    10921 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
    10981 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttaccac
    11041 catggattat aaagacgatg atgacaagca ccaccaccac caccacgaca aggattgtga
    11101 aatgaaacgc accacactgg acagcccttt ggggaagctg gagctgtctg gttgtgagca
    11161 gggtctgcac gaaataattt ttctgggcaa ggggacgtct gcagctgatg ccgtggaggt
    11221 cccagccccc gctgcggttc tcggaggtcc ggagcccctg atacaggcta cagcctggct
    11281 gaatgcctat ttccaccagc ccgaggctat cgaagagttc cccgtgccgg ctcttcacca
    11341 tcccgttttc cagcaagagt cgttcaccag acaggtgtta tggaagctgc tgaaggttgt
    11401 gaaattcgga gaagtgattt ctgagtcaca cttagcagcc ctggtaggca accccgcagc
    11461 cacggcagca gtgaatacgg cactggatgg caatcctgtc cctatcctga tcccgtgcca
    11521 cagagtggtc cagggggatt cggatgtggg gccgtacctt ggtggactgg ccgtgaagga
    11581 atggcttctg gcccatgaag gccaccggtt ggggaagcca ggcttgggat tcgaactgga
    11641 ggtgctgttc cagggccccg gtaccgccat cacaagtttg tacaaaaaag caggcaccat
    11701 ggcggcggcg tctgtctctg cggcttctgg ttctcacttg tcgaacagct ttgctgagcc
    11761 atcaaggtct aatggaagca tggttcggca ttcttcatct ccatatgtag tatatccttc
    11821 ggataagcct ttccttaata gtgatctacg acgctcccca agtaagccta cacttgccta
    11881 tccagaaagc aacagcagag ccatattttc tgctcttaag aatcttcaag ataagattcg
    11941 acgcttggaa cttgagagga ttcaggcaga agaaagtgtg aaaaccttgt ctagagaaac
    12001 aattgaatat aagaaagtac tggatgaaca gatacaagaa agggagaatt caaagaatga
    12061 ggaatcaaag cacaatcaag aactgacatc tcagttgtta gctgcagaaa ataaatgcaa
    12121 tctattagaa aaacaattgg aatacatgcg aaatatgata aagcatgccg aaatggagag
    12181 gacatctgtc ttagagaaac aagtttccct agaaagagaa cgacaacatg atcaaacaca
    12241 tgttcagagc caacttgaaa aattggatct tcttgaacag gagtataaca aacttaccac
    12301 aatgcaggcc cttgcagaaa aaaaaatgca agagttggaa gcaaaactcc atgaagaaga
    12361 acaggaaagg aaacgcatgc aagctaaggc agctgagttg cagactggtc tagaaacaaa
    12421 tagacttatc tttgaagata aggcaactcc gtgtgttccc aatgcaagaa gaattaaaaa
    12481 aaagaagtca aaaccaccag aaaaggtgta gaacccagct ttc
//
