LOCUS       sequence-276347-       13026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep128 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-Cep128
FEATURES             Location/Qualifiers
     source          1..13026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2708..4639)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGXXGGAGAXGGAGGAGAGG
                     GAGGAGGAGAGGGAGAGGGAGGAGAGGGXGGAGGAGAGGGAGAGGAGGAGGAGAGGAGA
                     GGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAG
                     AGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRG
                     RGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRR
                     PPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDG
                     GRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKT
                     SLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEV
                     LKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDG
                     DEGEEGQE"
     primer_bind     complement(5074..5092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5160..5264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5265..5333,5334..6125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5483..5502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6296..6884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6785..6804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7073..7093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7091..7388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7410..7808
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7852..7986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8124..8143)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8420..8681
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8798..9370
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9378..9397
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9425..9444
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9425..9443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9454..10074
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9527..9546)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10116..10237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10292..10671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10672..10875
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10826..10846
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10877..10895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10898..10916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10926..10950
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11050..11073
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11074..11091
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11641..11664
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11677..11701
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13016..13026,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     3841 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     3901 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     3961 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc
     4021 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctscccct cctcctgctc
     4081 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     4141 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tnntgctcct gcccctcctn
     4201 nnnnncctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     4261 ctgctcctgc ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     4321 ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac
     4381 cgtgggtccc tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc
     4441 tatgtcttgg ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc
     4501 gtcctcttcc ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc
     4561 cggagccttc tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac
     4621 ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct ttattagacg
     4681 acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca
     4741 tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca
     4801 tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga
     4861 tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt
     4921 agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct
     4981 actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag
     5041 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     5101 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     5521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     5821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6421 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6901 cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta
     6961 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     7021 tcataatggg gaaggccatc cagcctcgcg tcgaactaga tgatccggct gtggaatgtg
     7081 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7141 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7201 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7261 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7321 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7381 ttttttggag ggtgaccgcc acgaccggta tggccaagcc tttgtctcaa gaagaatcca
     7441 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     7501 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     7561 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     7621 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     7681 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     7741 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     7801 agggctaacg gtccgacgcg gcccgacggg tccgaggggg gtcgacctcg aaacttgttt
     7861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7981 tggatctgat ccgaacccct tcctcgacca attctcatgt ttgacagctt atcatcgcag
     8041 atccgggcaa cgttgttgcc attgctcgac caattctcat gtttgacagc ttatcatcgc
     8101 agatccgtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8161 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     8221 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     8281 ctgcttcgcg atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg
     8341 cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc
     8401 ttttgcatag ggagggggaa atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     8461 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     8521 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     8581 ttggacgaac cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata
     8641 caataaacgc catttgacca ttcaccacat tggtgtgcac ctccaagctg gccggggatc
     8701 ctctagggcc tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt
     8761 ttgcaaaaag ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg
     8821 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     8881 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     8941 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     9001 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     9061 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     9121 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     9181 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat
     9241 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     9301 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt
     9361 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     9421 attgtaatac gactcactat agggcgaatt gatatgtcta gattagataa aagtaaagtg
     9481 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
     9541 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg
     9601 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct
     9661 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct
     9721 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag
     9781 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat
     9841 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa
     9901 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta
     9961 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc
    10021 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac
    10081 agcggatccc gggaattcag atcttattaa agcagaactt gtttattgca gcttataatg
    10141 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10201 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg aatccatggc
    10261 gcagaactgg taggtatgga agatccgcgt tgacattgat tattgactag ttattaatag
    10321 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    10381 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    10441 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    10501 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    10561 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    10621 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    10681 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    10741 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    10801 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    10861 tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt
    10921 cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
    10981 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt
    11041 accaccatgg attataaaga cgatgatgac aagcaccacc accaccacca cgacaaggat
    11101 tgtgaaatga aacgcaccac actggacagc cctttgggga agctggagct gtctggttgt
    11161 gagcagggtc tgcacgaaat aatttttctg ggcaagggga cgtctgcagc tgatgccgtg
    11221 gaggtcccag cccccgctgc ggttctcgga ggtccggagc ccctgataca ggctacagcc
    11281 tggctgaatg cctatttcca ccagcccgag gctatcgaag agttccccgt gccggctctt
    11341 caccatcccg ttttccagca agagtcgttc accagacagg tgttatggaa gctgctgaag
    11401 gttgtgaaat tcggagaagt gatttctgag tcacacttag cagccctggt aggcaacccc
    11461 gcagccacgg cagcagtgaa tacggcactg gatggcaatc ctgtccctat cctgatcccg
    11521 tgccacagag tggtccaggg ggattcggat gtggggccgt accttggtgg actggccgtg
    11581 aaggaatggc ttctggccca tgaaggccac cggttgggga agccaggctt gggattcgaa
    11641 ctggaggtgc tgttccaggg ccccggtacc gccatcacaa gtttgtacaa aaaagcaggc
    11701 ttaatggcag aatcatccag cgaatcagat cacttccgct gtcgtgaccg attgagtcca
    11761 tgggctgcca gatcaacgca caggggaact cgaagtcttc ctacagtaga agttaccgag
    11821 aaggtcaaca ctataacaag tactttacag gataccagtc ggaacctgcg acaagtggac
    11881 cagatgcttg gacgataccg agaatacagt aatggacagg cgggtgcgat agaacattta
    11941 aaagaaagct tggaacaatc aatcgaccaa ctccggagtc aacgtttatt gagaaactca
    12001 ggagggagaa gtatttctgt tacaagtttg agtgcaagtg accttgatgg tggcactggg
    12061 tcagagctcc atcattttcc acctacctca cctctcaagg actatgggga tccacagggt
    12121 attaaacgaa tgagatcaag aactggtgtc cggtttgttc aagagactga tgatatgact
    12181 cagcttcatg gttttcatca gtctcttcga gacctcagca gtgaacaaat tcgccttgga
    12241 gatgatttca acagggagct ttccagaagg agccggtcag atgccgaaac aaaaagggct
    12301 ttggaagaat tgactgaaaa acttaatgaa gcccagaaac aagaagtggt ttcagatcgg
    12361 gtggagcggc ggcttcagga actggagaga gagatgcgca cagaaaggga gctggtggaa
    12421 agacgccagg atcaactggg actcatgtcc ctgcagctac aggaggcact aaagaaacaa
    12481 gaagctaaag cagatgagca tgagggggca ataaaaaata agctaagaca aactgaaact
    12541 gagaagaatc aacttgaaca ggaattggag ctatctcgaa ggttattgaa tcaatcagaa
    12601 ggcagccgag aaacactttt gcatcaggta gaagaactgc gtacacaact tacgaaagca
    12661 gaaggtgatc gaaagggttt acagcatcaa gtatctcaga tttccaagca acagtcaaac
    12721 tatcaggatg aacaagggga ggactggaga tttaggagag gggttgagcg ggaaaaacag
    12781 gacctggaga agcaaatgtc agatttgaga gtgcagctga acttcagcgc aatggcatct
    12841 gagttagagg aagtgaaacg gtgcatggag agaaaagaca aggagagagc acatttggca
    12901 tcacaagtag agcacatcaa tagaaaatgt cttttaaatt tggttcaaga tttggattgt
    12961 aaagataatg aaatattgac atactcttta caaagccctc tacatgttta atgataccca
    13021 gctttc
//
