LOCUS       sequence-276375-       12608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression cassette for R recombinase in T-DNA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12608)
  AUTHORS   Cody JP, Graham ND, Zhao C, Swyers NC, Birchler JA
  TITLE     Site-specific recombinase genome engineering toolkit in maize.
  JOURNAL   Plant Direct. 2020 Mar 9;4(3):e00209. doi: 10.1002/pld3.209. 
            eCollection 2020 Mar.
  PUBMED    32166212
REFERENCE   2  (bases 1 to 12608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-03-9"; volume: "4(3):e00209. doi"; pages: " 
            10.1002/pld3.209. eCollection 2020 Mar"
COMMENT     Sequence Label: pTF-R
FEATURES             Location/Qualifiers
     source          1..12608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6618..6640
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6632..6649
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6633..6649
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7219..7767)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7919..8263)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7955..7975)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        8361..10353
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             11849..11869
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11895..12147
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12168..12184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12168..12184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12181..12203)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12192..12208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12216..12222,12223..12240,12241..12246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12261..12282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12440..12464
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     6541 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     6601 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctaat
     6661 tcgcttcaag acgtgctcaa atcactattt ccacacccct atatttctat tgcactccct
     6721 tttaactgtt ttttattaca aaaatgccct ggaaaatgca ctcccttttt gtgtttgttt
     6781 ttttgtgaaa cgatgttgtc aggtaattta tttgtcagtc tactatggtg gcccattata
     6841 ttaatagcaa ctgtcggtcc aatagacgac gtcgattttc tgcatttgtt taaccacgtg
     6901 gattttatga cattttatat tagttaattt gtaaaaccta cccaattaaa gacctcatat
     6961 gttctaaaga ctaatactta atgataacaa ttttctttta gtgaagaaag ggataattag
     7021 taaatatgga acaagggcag aagatttatt aaagccgcgt aagagacaac aagtaggtac
     7081 gtggagtgtc ttaggtgact tacccacata acataaagtg acattaacaa acatagctaa
     7141 tgctcctatt tgaatagtgc atatcagcat accttattac atatagatag gagcaaactc
     7201 tagctagatt gttgagcaga tctcggtgac gggcaggacc ggacggggcg gtaccggcag
     7261 gctgaagtcc agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc
     7321 ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt
     7381 gggcagcccg atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag
     7441 gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt
     7501 cgactcggcc gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc
     7561 ggcgacctcg ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag
     7621 gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc
     7681 gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat
     7741 gtcggccggg cgtcgttctg ggctcatggt agatcccccg ttcgtaaatg gtgaaaattt
     7801 tcagaaaatt gcttttgctt taaaagaaat gatttaaatt gctgcaatag aagtagaatg
     7861 cttgattgct tgagattcgt ttgttttgta tatgttgtgt tgagaattaa ttctcgaggt
     7921 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     7981 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     8041 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     8101 actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat ggcatttgta
     8161 ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg ggcaatggaa
     8221 tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc
     8281 tgagactgta tctttgatat ttttggagta gacaagtgtg tcgtgctcca ccatgttgac
     8341 cggcatgcaa gcttgcatgc ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga
     8401 taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg
     8461 tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat
     8521 aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac
     8581 atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt
     8641 gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt
     8701 tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta
     8761 catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt
     8821 tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat
     8881 accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc
     8941 agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc
     9001 gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga
     9061 gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag
     9121 cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta
     9181 cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag
     9241 acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa
     9301 ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc
     9361 nccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt
     9421 agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag
     9481 cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt
     9541 ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc gatttcatga
     9601 ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc aatatatgcc
     9661 gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg tgatgatgtg
     9721 gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta cctggtggat
     9781 ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga attgaagatg
     9841 atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt actgatgcat
     9901 atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc
     9961 attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt attaattttg
    10021 gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga tggaaatatc
    10081 gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca tgatggcata
    10141 tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata aacaagtatg
    10201 ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca gctatatgtg
    10261 gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt cttttgtcga
    10321 tgctcaccct gttgtttggt gttacttctg caggtcgact ctagaggatc cccgggccat
    10381 ggcattgacc aaggacactg aaatttcaac aattaacaga caaatgtccg acttcagcga
    10441 gcttagccaa atccttcccc ttcaccaaat ctctaagatc aaggacattt tggagaatga
    10501 gaaccctctc ccaaaggaaa agctcgcctc ccacctgacc atgatcattt tgatggccaa
    10561 tttggccagt cagaaacgca aggacgttcc tgtcaagcgt tccactttct tgaagtacca
    10621 gcgctctatt tccaagacat tgcagtacga ctcttccacc aagaccgttt cctttgagta
    10681 ccacttgaaa gacccaagca agctcatcaa aggactggag gatgttgtga gcccctatcg
    10741 cttcgttgtg ggtgtgcacg agaagcctga tgacgtcatg agccacttgt ctgctgtcca
    10801 catgcgcaag gaggcaggtc gtaagagaga cttgggtaac aagattaacg acgagatcac
    10861 taaaatcgct gaaacacagg aaactatttg gggattcgtc ggaaagacta tggacctcat
    10921 cgaagctaga accactcgtc ctacaacaaa ggccgcatac aaccttttgc ttcaggccac
    10981 cttcatgaac tgttgcagag ctgacgactt gaagaatacc gacatcaaga ccttcgaagt
    11041 tattccagac aagcaccttg gcagaatgct ccgtgctttc gtcccagaga ctaagactgg
    11101 tactcgtttc gtctacttct tcccttgcaa aggacgttgc gacccactgt tggctctcga
    11161 ttcctacctc caatggaccg atcctattcc caaaactaga actactgatg aagacgccag
    11221 atacgattac cagcttctca ggaacagcct gcttggctca tacgacggat tcatctctaa
    11281 gcaatccgat gagagcatct ttaagattcc caatggacca aaggctcact tgggcaggca
    11341 tgtgaccgct tcataccttt ccaacaacga gatggataag gaggccaccc tctacggtaa
    11401 ttggtctgcc gctcgtgagg aaggcgtctc ccgtgtggct aaggctcgtt acatgcacac
    11461 catcgagaag agcccacctt cttacttgtt cgccttcctt tctggcttct acaacatcac
    11521 cgccgaaagg gcttgcgagt tggttgaccc aaacagcaac ccttgcgagc aagacaagaa
    11581 cattcctatg atctccgaca tcgagacctt gatggctcgt tacggaaaga acgccgagat
    11641 cattcctatg gacgtgttgg ttttcttgag cagctacgcc aggtttaaga acaacgaagg
    11701 taaggagtat aagttgcaag ctcgcagctc ccgtggcgtc ccagacttcc cagacaatgg
    11761 tagaactgcc ctttacaacg cattgaccgc tgcccatgtg aagcgcagaa agattagcat
    11821 tgtcgttgga cgtagcattg acacctcccc taagaaaaag cggaaagtgt gaaaagaagg
    11881 agtgcgtcga agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    11941 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    12001 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    12061 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    12121 gcgcggtgtc atctatgtta ctagatcgag ctcgaattcg taatcatgtc atagctgttt
    12181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    12241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    12301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    12361 gggagaggcg gtttgcgtat tggagcttga gcttggatca gattgtcgtt tcccgccttc
    12421 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
    12481 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
    12541 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttaaag tactttaaag
    12601 tactttaa
//
