LOCUS       sequence-276376-       12651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression cassette for Cre recombinase in T-DNA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12651)
  AUTHORS   Gaeta RT, Masonbrink RE, Zhao C, Sanyal A, Krishnaswamy L, Birchler 
            JA
  TITLE     In vivo modification of a maize engineered minichromosome.
  JOURNAL   Chromosoma. 2013 Jun;122(3):221-32. doi: 10.1007/s00412-013-0403-3. 
            Epub 2013 Mar 22.
  PUBMED    23519820
REFERENCE   2  (bases 1 to 12651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00412-013-0403-3"; journalName: "Chromosoma"; date: 
            "2013-06"; volume: "122"; issue: "3"; pages: "221-32"
COMMENT     Sequence Label: pTFUbiCre
FEATURES             Location/Qualifiers
     source          1..12651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1188..1817
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2246..3319
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3385..3579
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3815..3837)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3923..4063
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3978..3997
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4078..4095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4249..4837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4329..4348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5083..5874)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6395..6419
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6619..6641
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6633..6650
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6634..6650
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7220..7768)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7920..8264)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7956..7976)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        8362..10354
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             10388..11419
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTTGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     terminator      11597..11849
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12212..12228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12212..12228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12225..12247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12236..12252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12260..12266,12267..12284,12285..12290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12305..12326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12484..12508
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
       61 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
      121 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
      181 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
      241 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
      301 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
      361 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
      421 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
      481 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
      541 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
      601 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
      661 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
      721 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
      781 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
      841 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
      901 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
      961 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc
     1021 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     1081 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     1141 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     1201 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     1261 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     1321 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     1381 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     1441 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     1501 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     1561 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     1621 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     1681 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     1741 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     1801 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     1861 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     1921 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     1981 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     2041 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     2101 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     2161 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     2221 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     2281 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     2341 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     2401 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     2461 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     2521 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     2581 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     2641 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     2701 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     2761 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     2821 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     2881 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     2941 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     3001 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     3061 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     3121 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     3181 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     3241 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     3301 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     3361 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     3421 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     3481 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     3541 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     3601 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     3661 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     3721 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     3781 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3841 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3901 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     3961 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4021 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     4081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4681 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat
     4921 atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac
     4981 ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taacgcttga gttaagccgc
     5041 gccgcgaagc ggcgtcggct tgaacgaatt tctagctaga cattatttgc cgactacctt
     5101 ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa
     5161 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
     5221 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
     5281 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
     5341 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
     5401 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
     5461 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
     5521 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
     5581 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
     5641 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     5701 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
     5761 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     5821 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc ccatgatgtt
     5881 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     5941 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     6001 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     6121 cgaacaggct tatgtccact gggttcgtgc ccgaattgat cacaggcagc aacgctctgt
     6181 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     6241 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     6301 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     6361 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     6421 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg
     6481 ccgaattgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6541 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6601 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctaa
     6661 ttcgcttcaa gacgtgctca aatcactatt tccacacccc tatatttcta ttgcactccc
     6721 ttttaactgt tttttattac aaaaatgccc tggaaaatgc actccctttt tgtgtttgtt
     6781 tttttgtgaa acgatgttgt caggtaattt atttgtcagt ctactatggt ggcccattat
     6841 attaatagca actgtcggtc caatagacga cgtcgatttt ctgcatttgt ttaaccacgt
     6901 ggattttatg acattttata ttagttaatt tgtaaaacct acccaattaa agacctcata
     6961 tgttctaaag actaatactt aatgataaca attttctttt agtgaagaaa gggataatta
     7021 gtaaatatgg aacaagggca gaagatttat taaagccgcg taagagacaa caagtaggta
     7081 cgtggagtgt cttaggtgac ttacccacat aacataaagt gacattaaca aacatagcta
     7141 atgctcctat ttgaatagtg catatcagca taccttatta catatagata ggagcaaact
     7201 ctagctagat tgttgagcag atctcggtga cgggcaggac cggacggggc ggtaccggca
     7261 ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg
     7321 cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt
     7381 tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca
     7441 ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg
     7501 tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc
     7561 cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga
     7621 ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct
     7681 cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga
     7741 tgtcggccgg gcgtcgttct gggctcatgg tagatccccc gttcgtaaat ggtgaaaatt
     7801 ttcagaaaat tgcttttgct ttaaaagaaa tgatttaaat tgctgcaata gaagtagaat
     7861 gcttgattgc ttgagattcg tttgttttgt atatgttgtg ttgagaatta attctcgagg
     7921 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     7981 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
     8041 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     8101 cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga tggcatttgt
     8161 aggagccacc ttccttttcc actatcttca caataaagtg acagatagct gggcaatgga
     8221 atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc ctttggtctt
     8281 ctgagactgt atctttgata tttttggagt agacaagtgt gtcgtgctcc accatgttga
     8341 ccggcatgca agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag
     8401 ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt
     8461 gtttgaagtg cagtttatct atctttatac atatatttaa actttactct acgaataata
     8521 taatctatag tactacaata atatcagtgt tttagagaat catataaatg aacagttaga
     8581 catggtctaa aggacaattg agtattttga caacaggact ctacagtttt atctttttag
     8641 tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt
     8701 ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt
     8761 acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt
     8821 ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa
     8881 taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc
     8941 cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg
     9001 cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg
     9061 agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga
     9121 gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct
     9181 acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata
     9241 gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca
     9301 accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct
     9361 cccccccccc ccntctctac cttctctaga tcggcgttcc ggtccatggt tagggcccgg
     9421 tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc cgtgctgcta
     9481 gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa cttgccagtg
     9541 tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat cgatttcatg
     9601 attttttttg tttcgttgca tagggtttgg tttgcccttt tcctttattt caatatatgc
     9661 cgtgcacttg tttgtcgggt catcttttca tgcttttttt tgtcttggtt gtgatgatgt
     9721 ggtctggttg ggcggtcgtt ctagatcgga gtagaattct gtttcaaact acctggtgga
     9781 tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat
     9841 gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca
     9901 tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt
     9961 cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt
    10021 ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat
    10081 cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat
    10141 atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat
    10201 gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt
    10261 ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg
    10321 atgctcaccc tgttgtttgg tgttacttct gcaggtcgac tctagaggat ccccgggtac
    10381 cgttgacatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
    10441 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
    10501 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
    10561 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
    10621 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
    10681 tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
    10741 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
    10801 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat
    10861 acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
    10921 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
    10981 cagaacgaaa acgctggtta gcaccacagg tgtagagaag gcacttagcc tgggggtaac
    11041 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
    11101 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
    11161 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
    11221 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
    11281 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
    11341 aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
    11401 gctggaagat ggcgattagt aagaattcgc atgcattcat caatattatt catgcgggga
    11461 aaggcaagat taatccaact ggcaaatcat ccagcgtgat tggtaacttc agttccagcg
    11521 acttgattcg ttttggtgct acccacgttt tcaataagga cgagatggtg gagtaaagaa
    11581 ggagtgcgtc gaagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
    11641 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
    11701 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
    11761 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
    11821 gcgcgcggtg tcatctatgt tactagatcg atcaaacttc ggcactgtgt aatgacgatg
    11881 agcaatcgag aggctgacta acaaaaggta tgcccaaaaa caacctctcc aaactgtttc
    11941 gaattggaag tttctgctca tgccgacagg cataacttag atattcgcgg gctattccca
    12001 ctaattcgtc ctgctggttt gcgccaagat aaatcagtgc atctccttac aagttcctct
    12061 gtcttgtgaa atgaactgct gactgccccc caagaaagcc tcctcatctc ccagttggcg
    12121 gcggctgata caccatcgaa aacccacgtc cgaacacttg atacatgtgc ctgagaaata
    12181 ggggtcgacc cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
    12241 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    12301 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    12361 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    12421 gtattggagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
    12481 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
    12541 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
    12601 acagggttcc cctcgggatc aaagtacttt aaagtacttt aaagtacttt a
//
