LOCUS       sequence-276377-       11927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Centrin2 
            N-terminally fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 11927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetDBl-CLIP-Centrin2
FEATURES             Location/Qualifiers
     source          1..11927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2404..4329)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(4764..4782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4850..4954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4955..5023,5024..5815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5173..5192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5986..6574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6475..6494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6763..6783)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6781..7078
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7100..7498
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7542..7676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7814..7833)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8110..8371
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8488..9060
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9068..9087
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9115..9134
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9115..9133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9144..9764
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9217..9236)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    9806..9927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        9982..10361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10362..10565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10516..10536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10567..10585
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10588..10606
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10616..10640
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10740..10763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10764..10781
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11331..11354
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11367..11391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(11917..11927,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3541 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     3601 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     3661 ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     3721 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     3781 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3841 tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
     3901 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     3961 ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     4021 tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc
     4081 tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg
     4141 ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc
     4201 ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc
     4261 tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc
     4321 gtcagacatg attcacacta aaagagatca atagacatct ttattagacg acgctcagtg
     4381 aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat
     4441 ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct
     4501 catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct
     4561 gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg
     4621 attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa
     4681 cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca
     4741 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4801 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4921 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4981 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5041 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5101 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5161 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     5221 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5281 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5341 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5401 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5461 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     5521 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5581 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5641 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5701 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5761 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5821 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5881 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6121 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6601 gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
     6661 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
     6721 gaaggccatc cagcctcgcg tcgaactaga tgatccggct gtggaatgtg tgtcagttag
     6781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6901 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7021 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7081 ggtgaccgcc acgaccggta tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     7141 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     7201 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     7261 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     7321 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     7381 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     7441 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaacg
     7501 gtccgacgcg gcccgacggg tccgaggggg gtcgacctcg aaacttgttt attgcagctt
     7561 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7621 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     7681 ccgaacccct tcctcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     7741 cgttgttgcc attgctcgac caattctcat gtttgacagc ttatcatcgc agatccgtat
     7801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     7861 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     7921 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     7981 atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg
     8041 ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag
     8101 ggagggggaa atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     8161 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     8221 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     8281 cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc
     8341 catttgacca ttcaccacat tggtgtgcac ctccaagctg gccggggatc ctctagggcc
     8401 tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag
     8461 ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
     8521 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
     8581 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
     8641 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
     8701 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
     8761 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
     8821 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
     8881 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc
     8941 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
     9001 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag
     9061 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac
     9121 gactcactat agggcgaatt gatatgtcta gattagataa aagtaaagtg attaacagcg
     9181 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
     9241 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
     9301 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
     9361 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
     9421 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
     9481 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
     9541 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
     9601 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     9661 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
     9721 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac agcggatccc
     9781 gggaattcag atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata
     9841 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9901 tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg aatccatggc gcagaactgg
     9961 taggtatgga agatccgcgt tgacattgat tattgactag ttattaatag taatcaatta
    10021 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
    10081 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
    10141 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
    10201 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
    10261 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
    10321 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
    10381 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
    10441 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
    10501 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
    10561 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
    10621 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
    10681 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt accaccatgg
    10741 attataaaga cgatgatgac aagcaccacc accaccacca cgacaaggat tgtgaaatga
    10801 aacgcaccac actggacagc cctttgggga agctggagct gtctggttgt gagcagggtc
    10861 tgcacgaaat aatttttctg ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag
    10921 cccccgctgc ggttctcgga ggtccggagc ccctgataca ggctacagcc tggctgaatg
    10981 cctatttcca ccagcccgag gctatcgaag agttccccgt gccggctctt caccatcccg
    11041 ttttccagca agagtcgttc accagacagg tgttatggaa gctgctgaag gttgtgaaat
    11101 tcggagaagt gatttctgag tcacacttag cagccctggt aggcaacccc gcagccacgg
    11161 cagcagtgaa tacggcactg gatggcaatc ctgtccctat cctgatcccg tgccacagag
    11221 tggtccaggg ggattcggat gtggggccgt accttggtgg actggccgtg aaggaatggc
    11281 ttctggccca tgaaggccac cggttgggga agccaggctt gggattcgaa ctggaggtgc
    11341 tgttccaggg ccccggtacc gccatcacaa gtttgtacaa aaaagcaggc ttaatggcct
    11401 ccaactttaa gaaggcaaac atggcatcaa gttctcagcg aaaaagaatg agccctaagc
    11461 ctgagcttac tgaagagcaa aagcaggaga tccgggaagc ttttgatctt ttcgatgcgg
    11521 atggaactgg caccatagat gttaaagaac tgaaggtggc aatgagggcc ctgggctttg
    11581 aacccaagaa agaagaaatt aagaaaatga taagtgaaat tgataaggaa gggacaggaa
    11641 aaatgaactt tggtgacttt ttaactgtga tgacccagaa aatgtctgag aaagatacta
    11701 aagaagaaat cctgaaagct ttcaagctct ttgatgatga tgaaactggg aagatttcgt
    11761 tcaaaaatct gaaacgcgtg gccaaggagt tgggtgagaa cctgactgat gaggagctgc
    11821 aggaaatgat tgatgaagct gatcgagatg gagatggaga ggtcagtgag caagagttcc
    11881 tgcgcatcat gaaaaagacc agcctctatt aatgataccc agctttc
//
