LOCUS       sequence-276380-       12765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            N-terminally fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetDBl-CLIP-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..12765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4765..4783)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4851..4955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4956..5024,5025..5816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5174..5193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5987..6575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6476..6495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6764..6784)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6782..7079
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7101..7499
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7543..7677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7815..7834)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8111..8372
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8489..9061
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9069..9088
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9116..9135
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9116..9134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9145..9765
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9218..9237)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    9807..9928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        9983..10362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10363..10566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10517..10537
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10568..10586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10589..10607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10617..10641
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10741..10764
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10765..10782
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11332..11355
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11368..11392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(12755..12765,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tscycctccn scccctccts ctccyscncc tcctgcccct cctgctcctg cccctcctsc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3541 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     3601 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     3661 ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     3721 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     3781 ccctcctgcc cctcctcctg ctcctgsccc tcctcctsct cctscccctc ctsccccntc
     3841 ctccccctcc tcctsctcct sctcctcctg cccctcctcc tsctcctgcc cctcctcctg
     3901 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
     3961 cccctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     4021 ctgcccctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc
     4081 ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg
     4141 gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc
     4201 cccgtcctcg tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt
     4261 ctggtccaga tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct
     4321 cgtcagacat gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt
     4381 gaatacaggg agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca
     4441 tggtcgctgt cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc
     4501 tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc
     4561 tgagcctcaa gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg
     4621 gattctcggg acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca
     4681 acggaagaaa agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac
     4741 atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4801 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     5221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5461 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     5521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6121 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6601 ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     6661 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     6721 ggaaggccat ccagcctcgc gtcgaactag atgatccggc tgtggaatgt gtgtcagtta
     6781 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6841 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6901 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6961 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7021 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7081 gggtgaccgc cacgaccggt atggccaagc ctttgtctca agaagaatcc accctcattg
     7141 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     7201 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     7261 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     7321 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     7381 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     7441 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaac
     7501 ggtccgacgc ggcccgacgg gtccgagggg ggtcgacctc gaaacttgtt tattgcagct
     7561 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7621 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga
     7681 tccgaacccc ttcctcgacc aattctcatg tttgacagct tatcatcgca gatccgggca
     7741 acgttgttgc cattgctcga ccaattctca tgtttgacag cttatcatcg cagatccgta
     7801 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     7861 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     7921 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     7981 gatgtacggg ccagatatac gcgtatctga ggggactagg gtgtgtttag gcgaaaagcg
     8041 gggcttcggt tgtacgcggt taggagtccc ctcaggatat agtagtttcg cttttgcata
     8101 gggaggggga aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     8161 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     8221 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     8281 ccactgaatt ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg
     8341 ccatttgacc attcaccaca ttggtgtgca cctccaagct ggccggggat cctctagggc
     8401 ctctgagcta ttccagaagt agtgaagagg cttttttgga ggcctaggct tttgcaaaaa
     8461 gctccggatc gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt
     8521 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
     8581 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
     8641 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
     8701 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
     8761 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
     8821 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
     8881 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat
     8941 catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata
     9001 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca
     9061 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgtaata
     9121 cgactcacta tagggcgaat tgatatgtct agattagata aaagtaaagt gattaacagc
     9181 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     9241 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     9301 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     9361 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     9421 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     9481 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     9541 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     9601 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     9661 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     9721 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgcgta cagcggatcc
     9781 cgggaattca gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat
     9841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9901 gtttgtccaa actcatcaat gtatcttatc atgtctggtc gaatccatgg cgcagaactg
     9961 gtaggtatgg aagatccgcg ttgacattga ttattgacta gttattaata gtaatcaatt
    10021 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
    10081 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
    10141 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
    10201 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
    10261 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
    10321 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
    10381 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
    10441 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
    10501 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
    10561 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
    10621 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
    10681 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct taccaccatg
    10741 gattataaag acgatgatga caagcaccac caccaccacc acgacaagga ttgtgaaatg
    10801 aaacgcacca cactggacag ccctttgggg aagctggagc tgtctggttg tgagcagggt
    10861 ctgcacgaaa taatttttct gggcaagggg acgtctgcag ctgatgccgt ggaggtccca
    10921 gcccccgctg cggttctcgg aggtccggag cccctgatac aggctacagc ctggctgaat
    10981 gcctatttcc accagcccga ggctatcgaa gagttccccg tgccggctct tcaccatccc
    11041 gttttccagc aagagtcgtt caccagacag gtgttatgga agctgctgaa ggttgtgaaa
    11101 ttcggagaag tgatttctga gtcacactta gcagccctgg taggcaaccc cgcagccacg
    11161 gcagcagtga atacggcact ggatggcaat cctgtcccta tcctgatccc gtgccacaga
    11221 gtggtccagg gggattcgga tgtggggccg taccttggtg gactggccgt gaaggaatgg
    11281 cttctggccc atgaaggcca ccggttgggg aagccaggct tgggattcga actggaggtg
    11341 ctgttccagg gccccggtac cgccatcaca agtttgtaca aaaaagcagg cttagtgcgt
    11401 gagtgcatct ccatccacgt tggccaggct ggtgtccaga ttggcaatgc ctgctgggag
    11461 ctctactgcc tggaacacgg catccagccc gatggccaga tgccaagtga caagaccatt
    11521 gggggaggag atgactcctt caacaccttc ttcagtgaga cgggcgctgg caagcacgtg
    11581 ccccgggctg tgtttgtaga cttggaaccc acagtcattg atgaagttcg cactggcacc
    11641 taccgccagc tcttccaccc tgagcagctc atcacaggca aggaagatgc tgccaataac
    11701 tatgcccgag ggcactacac cattggcaag gagatcattg accttgtgtt ggaccgaatt
    11761 cgcaagctgg ctgaccagtg caccggtctt cagggcttct tggttttcca cagctttggt
    11821 gggggaactg gttctgggtt cacctccctg ctcatggaac gtctctcagt tgattatggc
    11881 aagaagtcca agctggagtt ctccatttac ccagcacccc aggtttccac agctgtagtt
    11941 gagccctaca actccatcct caccacccac accaccctgg agcactctga ttgtgccttc
    12001 atggtagaca atgaggccat ctatgacatc tgtcgtagaa acctcgatat cgagcgccca
    12061 acctacacta accttaaccg ccttattagc cagattgtgt cctccatcac tgcttccctg
    12121 agatttgatg gagccctgaa tgttgacctg acagaattcc agaccaacct ggtgccctac
    12181 ccccgcatcc acttccctct ggccacatat gcccctgtca tctctgctga gaaagcctac
    12241 catgaacagc tttctgtagc agagatcacc aatgcttgct ttgagccagc caaccagatg
    12301 gtgaaatgtg accctcgcca tggtaaatac atggcttgct gcctgttgta ccgtggtgac
    12361 gtggttccca aagatgtcaa tgctgccatt gccaccatca aaaccaagcg cagcatccag
    12421 tttgtggatt ggtgccccac tggcttcaag gttggcatca actaccagcc tcccactgtg
    12481 gtgcctggtg gagacctggc caaggtacag agagctgtgt gcatgctgag caacaccaca
    12541 gccattgctg aggcctgggc tcgcctggac cacaagtttg acctgatgta tgccaagcgt
    12601 gcctttgttc actggtacgt gggtgagggg atggaggaag gcgagttttc agaggcccgt
    12661 gaagatatgg ctgcccttga gaaggattat gaggaggttg gtgtggattc tgttgaagga
    12721 gagggtgagg aagaaggaga ggaatactaa tgatacccag ctttc
//
