LOCUS       sequence-276401-       12116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep27 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetDBl-CLIP-Cep27
FEATURES             Location/Qualifiers
     source          1..12116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2404..4332)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGXGXGGGAGGAGGAGAGGGAGAGGGAGGXGXGGGG
                     GGXGGAGAGGGAGAGGGAGGAGAGGAGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAG
                     GGAGGAGGAGAGGAGAGGAGAGXAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGA
                     GGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGR
                     GGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRP
                     PPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGG
                     RRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTS
                     LYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL
                     KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGD
                     EGEEGQE"
     primer_bind     complement(4767..4785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4853..4957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4958..5026,5027..5818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5176..5195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5989..6577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6478..6497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6766..6786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6784..7081
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7103..7501
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7545..7679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7817..7836)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8113..8374
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8491..9063
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9071..9090
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9118..9137
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9118..9136
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9147..9767
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9220..9239)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    9809..9930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        9985..10364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10365..10568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10519..10539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10570..10588
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10591..10609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10619..10643
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10743..10766
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10767..10784
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11334..11357
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11370..11394
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(12106..12116,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3541 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccy
     3601 wcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     3661 ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     3721 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcycctgccc ctcctgctcc
     3781 tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctscccc
     3841 tccyccycct cctcctsctc ctscccctcc tgcccctcct cctgctcctg cccctcctcc
     3901 tgctcctgcc cctcctgccc ctcctgcccc tcctcctsct cctscccctc ctcctgctcc
     3961 tgcccctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
     4021 tcctgcccct cctgctcctg cccctcccgc tcctgctcct gctcctgttc caccgtgggt
     4081 ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct
     4141 tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct
     4201 tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc
     4261 ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc
     4321 ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca
     4381 gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt
     4441 catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt
     4501 cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt
     4561 cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg
     4621 gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg
     4681 caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa
     4741 acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4801 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4861 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4921 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4981 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5041 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5101 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5161 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     5221 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5281 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5341 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5401 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5461 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     5521 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5581 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5641 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5701 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5761 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5821 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5881 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5941 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6121 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6601 acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct
     6661 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     6721 ggggaaggcc atccagcctc gcgtcgaact agatgatccg gctgtggaat gtgtgtcagt
     6781 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6841 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6901 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6961 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7021 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7081 gagggtgacc gccacgaccg gtatggccaa gcctttgtct caagaagaat ccaccctcat
     7141 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     7201 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     7261 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     7321 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     7381 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     7441 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     7501 acggtccgac gcggcccgac gggtccgagg ggggtcgacc tcgaaacttg tttattgcag
     7561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     7681 gatccgaacc ccttcctcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     7741 caacgttgtt gccattgctc gaccaattct catgtttgac agcttatcat cgcagatccg
     7801 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     7861 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     7921 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     7981 gcgatgtacg ggccagatat acgcgtatct gaggggacta gggtgtgttt aggcgaaaag
     8041 cggggcttcg gttgtacgcg gttaggagtc ccctcaggat atagtagttt cgcttttgca
     8101 tagggagggg gaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8161 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8221 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8281 aaccactgaa ttccgcattg cagagatatt gtatttaagt gcctagctcg atacaataaa
     8341 cgccatttga ccattcacca cattggtgtg cacctccaag ctggccgggg atcctctagg
     8401 gcctctgagc tattccagaa gtagtgaaga ggcttttttg gaggcctagg cttttgcaaa
     8461 aagctccgga tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc
     8521 tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt
     8581 agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac
     8641 tttctactct gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt
     8701 tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca
     8761 gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat attatattgt
     8821 acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg
     8881 catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc
     8941 atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa
     9001 tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta
     9061 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgtaa
     9121 tacgactcac tatagggcga attgatatgt ctagattaga taaaagtaaa gtgattaaca
     9181 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
     9241 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
     9301 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
     9361 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
     9421 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
     9481 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
     9541 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
     9601 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
     9661 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
     9721 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgcg tacagcggat
     9781 cccgggaatt cagatcttat taaagcagaa cttgtttatt gcagcttata atggttacaa
     9841 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9901 tggtttgtcc aaactcatca atgtatctta tcatgtctgg tcgaatccat ggcgcagaac
     9961 tggtaggtat ggaagatccg cgttgacatt gattattgac tagttattaa tagtaatcaa
    10021 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
    10081 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
    10141 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    10201 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    10261 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    10321 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    10381 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    10441 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    10501 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    10561 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
    10621 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
    10681 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttaccacca
    10741 tggattataa agacgatgat gacaagcacc accaccacca ccacgacaag gattgtgaaa
    10801 tgaaacgcac cacactggac agccctttgg ggaagctgga gctgtctggt tgtgagcagg
    10861 gtctgcacga aataattttt ctgggcaagg ggacgtctgc agctgatgcc gtggaggtcc
    10921 cagcccccgc tgcggttctc ggaggtccgg agcccctgat acaggctaca gcctggctga
    10981 atgcctattt ccaccagccc gaggctatcg aagagttccc cgtgccggct cttcaccatc
    11041 ccgttttcca gcaagagtcg ttcaccagac aggtgttatg gaagctgctg aaggttgtga
    11101 aattcggaga agtgatttct gagtcacact tagcagccct ggtaggcaac cccgcagcca
    11161 cggcagcagt gaatacggca ctggatggca atcctgtccc tatcctgatc ccgtgccaca
    11221 gagtggtcca gggggattcg gatgtggggc cgtaccttgg tggactggcc gtgaaggaat
    11281 ggcttctggc ccatgaaggc caccggttgg ggaagccagg cttgggattc gaactggagg
    11341 tgctgttcca gggccccggt accgccatca caagtttgta caaaaaagca ggcaccatgg
    11401 ccgctgccaa cccgtgggac ccggcgtccg cgcctaacgg cgctgggcta gtgctaggcc
    11461 acttcatagc ttcggggatg gtcaatcagg agatgttaaa catgtctaag aaaacagttt
    11521 cttgttttgt gaacttcacc agactacagc agatcacaaa tattcaagct gaaatctacc
    11581 agaaaaacct ggaaattgaa ctcctgaaac tagaaaaaga tacagcagat gttgttcatc
    11641 ctttcttttt ggctcagaag tgtcatactc tgcaaagcat gaataatcat ttggaagcag
    11701 tgctgaaaga gaagagatcc cttaggcaaa gactgttgaa acccatgtgc caggaaaact
    11761 tacctattga agctgtttat cacagatata tggtacattt gctggagttg gctgtgactt
    11821 tcattgagag attagaaacc caccttgaaa caattagaaa tattcctcat ttagctgcaa
    11881 atctaaagaa aatgaaccag gctttagcaa agatggatat attggtgact gagacagaag
    11941 aactggcaga gaatatactc aagtggcgta aacaacaaaa cgaagtttcg tcttgtatcc
    12001 ccaaaatatt agctgaagaa agttatcttt ataaacatga tattataatg cctcctttac
    12061 cttttacttc taaagttcat gtccaaacta ttaatgccaa gtagaaccca gctttc
//
