LOCUS       sequence-276407-       13351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep72 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13351)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetDBl-CLIP-Cep72
FEATURES             Location/Qualifiers
     source          1..13351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4766..4784)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4852..4956
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4957..5025,5026..5817)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5175..5194)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5988..6576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6477..6496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6765..6785)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6783..7080
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7102..7500
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7544..7678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7816..7835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8112..8373
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8490..9062
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9070..9089
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9117..9136
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9117..9135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9146..9766
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9219..9238)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    9808..9929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        9984..10363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10364..10567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10518..10538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10569..10587
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10590..10608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10618..10642
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10742..10765
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10766..10783
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11333..11356
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11369..11393
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13341..13351,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
     3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     3421 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgccccycc
     3541 tgctcctgcc cctccntgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     3601 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctccc tgcccctcct
     3661 gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct
     3721 gctcctgccc cycctcctgc tcctgcccct cctgcccctc ctccccctcc tcctgctcct
     3781 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     3841 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc cysctcctgc ccctcctccy
     3901 sctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     3961 gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     4021 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
     4081 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
     4141 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
     4201 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
     4261 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
     4321 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
     4381 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
     4441 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
     4501 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
     4561 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
     4621 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
     4681 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
     4741 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4801 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4861 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4921 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4981 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5041 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5101 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5161 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5221 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5281 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5341 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5401 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5461 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5521 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5581 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5641 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5701 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5761 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5821 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5881 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5941 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6001 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6061 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6121 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6181 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6241 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6301 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6361 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6421 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6481 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6541 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6601 cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
     6661 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     6721 gggaaggcca tccagcctcg cgtcgaacta gatgatccgg ctgtggaatg tgtgtcagtt
     6781 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6841 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6901 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6961 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7021 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7081 agggtgaccg ccacgaccgg tatggccaag cctttgtctc aagaagaatc caccctcatt
     7141 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     7201 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     7261 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     7321 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     7381 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     7441 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     7501 cggtccgacg cggcccgacg ggtccgaggg gggtcgacct cgaaacttgt ttattgcagc
     7561 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7621 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     7681 atccgaaccc cttcctcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
     7741 aacgttgttg ccattgctcg accaattctc atgtttgaca gcttatcatc gcagatccgt
     7801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     7861 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     7921 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     7981 cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc
     8041 ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc gcttttgcat
     8101 agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     8161 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     8221 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     8281 accactgaat tccgcattgc agagatattg tatttaagtg cctagctcga tacaataaac
     8341 gccatttgac cattcaccac attggtgtgc acctccaagc tggccgggga tcctctaggg
     8401 cctctgagct attccagaag tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa
     8461 agctccggat cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct
     8521 ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta
     8581 gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact
     8641 ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt
     8701 cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag
     8761 attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta
     8821 cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc
     8881 atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca
     8941 tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat
     9001 actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac
     9061 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgtaat
     9121 acgactcact atagggcgaa ttgatatgtc tagattagat aaaagtaaag tgattaacag
     9181 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca
     9241 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct
     9301 cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg
     9361 ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag
     9421 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac
     9481 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata
     9541 tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca
     9601 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca
     9661 agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt
     9721 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt acagcggatc
     9781 ccgggaattc agatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa
     9841 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9901 ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgaatccatg gcgcagaact
     9961 ggtaggtatg gaagatccgc gttgacattg attattgact agttattaat agtaatcaat
    10021 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    10081 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    10141 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    10201 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    10261 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    10321 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    10381 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    10441 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    10501 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    10561 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
    10621 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
    10681 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttaccaccat
    10741 ggattataaa gacgatgatg acaagcacca ccaccaccac cacgacaagg attgtgaaat
    10801 gaaacgcacc acactggaca gccctttggg gaagctggag ctgtctggtt gtgagcaggg
    10861 tctgcacgaa ataatttttc tgggcaaggg gacgtctgca gctgatgccg tggaggtccc
    10921 agcccccgct gcggttctcg gaggtccgga gcccctgata caggctacag cctggctgaa
    10981 tgcctatttc caccagcccg aggctatcga agagttcccc gtgccggctc ttcaccatcc
    11041 cgttttccag caagagtcgt tcaccagaca ggtgttatgg aagctgctga aggttgtgaa
    11101 attcggagaa gtgatttctg agtcacactt agcagccctg gtaggcaacc ccgcagccac
    11161 ggcagcagtg aatacggcac tggatggcaa tcctgtccct atcctgatcc cgtgccacag
    11221 agtggtccag ggggattcgg atgtggggcc gtaccttggt ggactggccg tgaaggaatg
    11281 gcttctggcc catgaaggcc accggttggg gaagccaggc ttgggattcg aactggaggt
    11341 gctgttccag ggccccggta ccgccatcac aagtttgtac aaaaaagcag gcaccatggc
    11401 gcgggctggc cctcggctgg tgctgagcga ggaggcggtt cgggcgaaga gcggcttagg
    11461 gcctcaccgc gacctggctg agcttcagtc attgtctatt cctggaactt accaagagaa
    11521 gatcacccac ctgggacatt ctctgatgag tttaacaggt ctgaaatctt tggatctctc
    11581 gcgcaactcc ttggttagtc tggagggcat tcagtacctg actgcattgg agagtctcaa
    11641 tctctactac aactgcatct cctcgttggc agaagtgttt cggctccacg ccttaaccga
    11701 gctcgtggat gtggacttcc ggctgaaccc cgtggtgaag gttgagcctg actaccgcct
    11761 ttttgttgtg cacctgctcc ccaagctcca gcagctggac gatcgccccg tgagagcaag
    11821 cgagcggaag gcttcccgac tgcattttgc atcagaggac tcactcgact ccaaagagag
    11881 cgtcccagct tctttgaaag agggcagacc acaccacccc agagccaagt gcaccgaggc
    11941 cttggccaag cagagcctgg tcatggatgc ggatgacgag gcagtcctga acctcattgc
    12001 agagtgcgag tgggacctcg gcaggcctcc cgggagcacg agcttcagcc agaaggggcg
    12061 tgaggccgac tctcgtggtt cccaagaatc cagacatctg ttgagcccgc agttggtaca
    12121 gtaccagtgt ggggactctg ggaagcaggg ccgtgagacg aggaggagca gctgcagagg
    12181 gtgctgtctg gagaagatgc cttggagcca gctctgtgga gagcttccgc cactgtacgg
    12241 agcggagcca gaggcctccc gtgcccccag gccacacacg tacttcaccc cacacccaga
    12301 ctccatggat accgaggact cggcctcttc tcagaagttg gatttgtcag gagaaatggt
    12361 gcctggtccc ctgccagccc ccggaaagtg caggaagcga agaatgcctg ttggaagatt
    12421 ccagacgttt tcggaccagg agggtttggg ctgcccggag agaactcatg ggtcctccgt
    12481 gcccaaggag agcctgagca gacaggacag ctcagaaagc aggaacggga ggaccttgtc
    12541 tcagcctgag gcctcggaga ctgaggagca gaggtctcgg ggtgtgaccg acaccagaga
    12601 gccgtctccc gggtcacact cggctctacc cgggaagaag acggccctgc aggcggcgct
    12661 cctggagacg ctcttggacc tggtggacag gagctggggc ggctgcaggt ccctgcacag
    12721 caacgaggca ttccttgctc aggcaagaca catcttgtca tctgttgaag aattcacagc
    12781 agctcaggac agctctgcga tggtgggtga agatgtcggc tccctggctc tggagagtaa
    12841 gtccctgcaa agccgccttg ctgagcagca gcagcagcac gcccgggaga tgagcgaggt
    12901 gacggcggag ctgcaccaca cacacaagga gctggatgat ttgagacaac atttagataa
    12961 atctttggaa gagaacagta ggttaaaatc gcttttgttg agtatgaaaa aggaagtgaa
    13021 gagtgcagac actgcagcca cgttaaattt gcagatcgct ggacttcaaa caagtgtgaa
    13081 gaggctgtgt ggcgagattg tggaactgaa gcagcacctg gagcactacg acaagatcca
    13141 ggagctcacg cagatgctgc aggagagcca cagctccctg gtcagcacca atgaacacct
    13201 gctgcaggag ctgagccagg tgcgggcgca gcacagagcc gaggtggagc agatgcactg
    13261 gagctaccag gagctcaaga agaccatggc cctgtttcca cacagcagcg ccagccatgg
    13321 aggctgccag gcctgctaga acccagcttt c
//
