LOCUS       sequence-276411-        5544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of CD44 proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Mielgo A, van Driel M, Bloem A, Landmann L, Gunthert U
  TITLE     A novel antiapoptotic mechanism based on interference of Fas 
            signaling by CD44 variant isoforms.
  JOURNAL   Cell Death Differ. 2006 Mar;13(3):465-77. doi: 
            10.1038/sj.cdd.4401763.
  PUBMED    16167069
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/sj.cdd"; 
            journalName: "Cell Death Differ"; date: "2006-03"; volume: "13"; 
            issue: "3"; pages: "465-77"
COMMENT     Sequence Label: pPGK-T7/2-CD44v3 (fl)
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          550..1122
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1130..1149
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1177..1196
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1177..1195
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2441..2450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2447..2449,2450..2452,2453..3166)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2447..2449,2450..2452,2453..3163)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2753..2772)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3100..3121
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3200..3321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3237..3256)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3291..3310
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3363..3467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3468..3536,3537..4328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3686..3705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4499..5087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4988..5007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5241..5258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5412..5431)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg acccctaggc ttttgcaaaa agctccggat cgatcctgag
      541 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      601 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      661 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      721 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      781 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      841 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      901 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      961 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     1021 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     1081 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1141 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
     1201 ttccgctccg gacaccatgg acaagttttg gtggcacgca gcctggggac tctgcctcgt
     1261 gccgctgagc ctggcgcaga tcgatttgaa tataacctgc cgctttgcag gtgtattcca
     1321 cgtggagaaa aatggtcgct acagcatctc tcggacggag gccgctgacc tctgcaaggc
     1381 tttcaatagc accttgccca caatggccca gatggagaaa gctctgagca tcggatttga
     1441 gacctgcagg tatgggttca tagaagggca cgtggtgatt ccccggatcc accccaactc
     1501 catctgtgca gcaaacaaca caggggtgta catcctcaca tccaacacct cccagtatga
     1561 cacatattgc ttcaatgctt cagctccacc tgaagaagat tgtacatcag tcacagacct
     1621 gcccaatgcc tttgatggac caattaccat aactattgtt aaccgtgatg gcacccgcta
     1681 tgtccagaaa ggagaataca gaacgaatcc tgaagacatc taccccagca accctactga
     1741 tgatgacgtg agcagcggct cctccagtga aaggagcagc acttcaggag gttacatctt
     1801 ttacaccttt tctactgtac accccatccc agacgaagac agtccctgga tcaccgacag
     1861 cacagacaga atccctgcta ccagtacgtc ttcaaatacc atctcagcag gctgggagcc
     1921 aaatgaagaa gatgaagatg aaagagacag acacctcagt ttttctggat caggcattga
     1981 tgatgatgaa gattttatct ccagcaccag agaccaagac acattccacc ccagtggggg
     2041 gtcccatacc actcatggat ctgaatcaga tggacactca catgggagtc aagaaggtgg
     2101 agcaaacaca acctctggtc ctataaggac accccaaatt ccagaatggc tgatcatctt
     2161 ggcatccctc ttggccttgg ctttgattct tgcagtttgc attgcagtca acagtcgaag
     2221 aaggtgtggg cagaagaaaa agctagtgat caacagtggc aatggagctg tggaggacag
     2281 aaagccaagt ggactcaacg gagaggccag caagtctcag gaaatggtgc atttggtgaa
     2341 caaggagtcg tcagaaactc cagaccagtt tatgacagct gatgagacaa ggaacctgca
     2401 gaatgtggac atgaagattg gggtagatcc ccggccggtc gccaccatgg tgagcaaggg
     2461 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2521 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2581 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2641 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2701 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2761 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2821 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2881 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2941 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3001 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3061 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3121 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgagat
     3181 ccagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca aataaagcaa
     3241 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3301 caaactcatc aatgtatctt atcatgtctg gtcgagcagg tggcactttt cggggaaatg
     3361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5281 gcaaaccgcc tctccccgcg cgttggccga ttcatttcga cttctgaggc ggaaagaacc
     5341 aggccaatat gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
     5401 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5461 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5521 gtgccctgaa tgaacaattc tacc
//
