LOCUS       sequence-276414-        5337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of CD44 proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Mielgo A, van Driel M, Bloem A, Landmann L, Gunthert U
  TITLE     A novel antiapoptotic mechanism based on interference of Fas 
            signaling by CD44 variant isoforms.
  JOURNAL   Cell Death Differ. 2006 Mar;13(3):465-77. doi: 
            10.1038/sj.cdd.4401763.
  PUBMED    16167069
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/sj.cdd"; 
            journalName: "Cell Death Differ"; date: "2006-03"; volume: "13"; 
            issue: "3"; pages: "465-77"
COMMENT     Sequence Label: pPGK-T7/2-CD44v6 (-cyt)
FEATURES             Location/Qualifiers
     source          1..5337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          550..1122
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1130..1149
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1177..1196
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1177..1195
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2234..2243
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2240..2242,2243..2245,2246..2959)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2240..2242,2243..2245,2246..2956)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2546..2565)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2893..2914
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2993..3114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3030..3049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3084..3103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3156..3260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3261..3329,3330..4121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3479..3498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4292..4880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4781..4800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5034..5051
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5205..5224)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg acccctaggc ttttgcaaaa agctccggat cgatcctgag
      541 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      601 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      661 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      721 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      781 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      841 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      901 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      961 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     1021 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     1081 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1141 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
     1201 ttccgctccg gacaccatgg acaagttttg gtggcacgca gcctggggac tctgcctcgt
     1261 gccgctgagc ctggcgcaga tcgatttgaa tataacctgc cgctttgcag gtgtattcca
     1321 cgtggagaaa aatggtcgct acagcatctc tcggacggag gccgctgacc tctgcaaggc
     1381 tttcaatagc accttgccca caatggccca gatggagaaa gctctgagca tcggatttga
     1441 gacctgcagg tatgggttca tagaagggca cgtggtgatt ccccggatcc accccaactc
     1501 catctgtgca gcaaacaaca caggggtgta catcctcaca tccaacacct cccagtatga
     1561 cacatattgc ttcaatgctt cagctccacc tgaagaagat tgtacatcag tcacagacct
     1621 gcccaatgcc tttgatggac caattaccat aactattgtt aaccgtgatg gcacccgcta
     1681 tgtccagaaa ggagaataca gaacgaatcc tgaagacatc taccccagca accctactga
     1741 tgatgacgtg agcagcggct cctccagtga aaggagcagc acttcaggag gttacatctt
     1801 ttacaccttt tctactgtac accccatccc agacgaagac agtccctgga tcaccgacag
     1861 cacagacaga atccctgcta ccatccaggc aactcctagt agtacaacgg aagaaacagc
     1921 tacccagaag gaacagtggt ttggcaacag atggcatgag ggatatcgcc aaacacccaa
     1981 agaagactcc cattcgacaa cagggacagc tggagaccaa gacacattcc accccagtgg
     2041 ggggtcccat accactcatg gatctgaatc agatggacac tcacatggga gtcaagaagg
     2101 tggagcaaac acaacctctg gtcctataag gacaccccaa attccagaat ggctgatcat
     2161 cttggcatcc ctcttggcct tggctttgat tcttgcagtt tgcattgcag tcaacagaga
     2221 tcccaggccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2281 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2341 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2401 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     2461 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2521 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2581 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2641 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2701 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2761 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2821 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2881 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2941 ggacgagctg tacaagtaaa gcggccgcga gatccagatc ttattaaagc agaacttgtt
     3001 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3061 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3121 ctggtcgagc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3181 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3241 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3301 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3361 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3421 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3481 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3541 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3601 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3661 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3721 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3781 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3841 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3901 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3961 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4021 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4081 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4141 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4921 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4981 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5041 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5101 cgattcattt cgacttctga ggcggaaaga accaggccaa tatgggatcg gccattgaac
     5161 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5221 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5281 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaacaa ttctacc
//
