LOCUS       sequence-276426-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike)
            fused with sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-SARS-CoV-2-S-RBD-sfGFP
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(789..817)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg ccaccatgaa gaccatcatc gccctgagct
      121 acatcttctg cctggtgttc gccactaatc tttgtccgtt cggtgaggtt tttaacgcga
      181 caaggttcgc tagtgtatat gcttggaacc gaaagagaat ctccaattgc gtagctgatt
      241 actccgttct ctataacagt gcgtcctttt caacctttaa gtgttacggc gtttctccaa
      301 cgaagctgaa tgatctctgt tttacgaacg tgtatgctga ctctttcgtt atacgggggg
      361 acgaagtgag acagatagca ccaggtcaga ctgggaagat agcggattac aactataagt
      421 tgcccgatga ttttacgggg tgcgtaatcg catggaactc aaacaacctc gactccaaag
      481 taggtggtaa ttataattac ttgtatcgcc tgtttcgaaa gagcaatttg aagccttttg
      541 agcgggatat ttcaaccgaa atttaccaag caggcagtac gccatgtaac ggagtagagg
      601 gatttaattg ctactttcct cttcaatctt atggctttca accaacaaac ggagtggggt
      661 atcaacctta tagagtggta gtattgtcct ttgagctcct ccacgccccg gctacagttt
      721 gtgggcccaa aaagggatcc ggtgggtccg gtagtggtgg ttccaaagga gaagaacttt
      781 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      841 ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa actcaccctt aaatttattt
      901 gcactactgg aaaactacct gttccatggc caacacttgt cactactctg acctatggtg
      961 ttcaatgc
//
