LOCUS       sequence-276429-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike)
            fused with IgG1-Fc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-SARS-CoV-2-S-RBD-Fc
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgccaccatg aagaccatca tcgccctgag
      121 ctacatcttc tgcctggtgt tcgccactaa tctttgtccg ttcggtgagg tttttaacgc
      181 gacaaggttc gctagtgtat atgcttggaa ccgaaagaga atctccaatt gcgtagctga
      241 ttactccgtt ctctataaca gtgcgtcctt ttcaaccttt aagtgttacg gcgtttctcc
      301 aacgaagctg aatgatctct gttttacgaa cgtgtatgct gactctttcg ttatacgggg
      361 ggacgaagtg agacagatag caccaggtca gactgggaag atagcggatt acaactataa
      421 gttgcccgat gattttacgg ggtgcgtaat cgcatggaac tcaaacaacc tcgactccaa
      481 agtaggtggt aattataatt acttgtatcg cctgtttcga aagagcaatt tgaagccttt
      541 tgagcgggat atttcaaccg aaatttacca agcaggcagt acgccatgta acggagtaga
      601 gggatttaat tgctactttc ctcttcaatc ttatggcttt caaccaacaa acggagtggg
      661 gtatcaacct tatagagtgg tagtattgtc ctttgagctc ctccacgccc cggctacagt
      721 ttgtgggccc aaaaagggat ccgacaaaac tcacacatgc ccaccgtgcc cagcacctga
      781 actcctgggg ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat
      841 ctcccggacc cctgag
//
