LOCUS       sequence-276436-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the membrane (M) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 M
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(762..861)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(973..992)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gatttnattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg catggctgac tctaacggta ccatcaccgt
      121 ggaggaactg aagaagctgc tggagcagtg gaacctggtc atcggcttcc tgttcctgac
      181 ctggatctgc ctgctgcagt tcgcctacgc taaccgcaac aggttcctgt acatcatcaa
      241 gctgatcttc ctgtggctgc tgtggcctgt gaccctggct tgcttcgtgc tggctgccgt
      301 gtaccgcatc aactggatca ccggcggaat cgccatcgct atggcctgcc tggtgggcct
      361 gatgtggctg tcttacttca tcgctagctt caggctgttc gccagaaccc gttccatgtg
      421 gtctttcaac cccgagacca acatcctgct gaacgtgcct ctgcacggaa ccatcctgac
      481 cagaccactg ctggagagcg aactggtcat cggcgctgtg atcctgagag gacacctgcg
      541 tatcgccgga caccacctgg gtcgttgcga catcaaggac ctgcccaagg aaatcaccgt
      601 ggctaccagc cgcaccctgt cctactacaa gctgggagct tctcagagag tggctggtga
      661 ctctggtttc gctgcttact ctcgctacag gatcggtaac tacaagctga acaccgacca
      721 cagctcctct agcgacaaca tcgccctgct ggtgcagtaa tacccaactt tcttgtacaa
      781 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      841 tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct
      901 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
      961 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     1021 taaattccaa catgg
//
