LOCUS       sequence-276437-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the membrane (M) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 M
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      69..112
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    168..267
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(937..961)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcacaacgtt caaatccgnt cccggcggat ttgtcctact caggagagcn ntcacngaca
       61 aacaacagat aaaacgaaag gcccagtctt ccgactganc ctttcgtttt atttgatgcc
      121 tggcagttcc ntactctcgc gttaacgcta gcatggatct cgggccccaa ataatgattt
      181 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
      241 tgccaacttt gtacaaaaaa gttggcatgg ctgactctaa cggtaccatc accgtggagg
      301 aactgaagaa gctgctggag cagtggaacc tggtcatcgg cttcctgttc ctgacctgga
      361 tctgcctgct gcagttcgcc tacgctaacc gcaacaggtt cctgtacatc atcaagctga
      421 tcttcctgtg gctgctgtgg cctgtgaccc tggcttgctt cgtgctggct gccgtgtacc
      481 gcatcaactg gatcaccggc ggaatcgcca tcgctatggc ctgcctggtg ggcctgatgt
      541 ggctgtctta cttcatcgct agcttcaggc tgttcgccag aacccgttcc atgtggtctt
      601 tcaaccccga gaccaacatc ctgctgaacg tgcctctgca cggaaccatc ctgaccagac
      661 cactgctgga gagcgaactg gtcatcggcg ctgtgatcct gagaggacac ctgcgtatcg
      721 ccggacacca cctgggtcgt tgcgacatca aggacctgcc caaggaaatc accgtggcta
      781 ccagccgcac cctgtcctac tacaagctgg gagcttctca gagagtggct ggtgactctg
      841 gtttcgctgc ttactctcgc tacaggatcg gtaactacaa gctgaacacc gaccacagct
      901 cctctagcga caacatcgcc ctgctggtgc agtaataccc aactttcttg tacaaagttg
      961 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     1021 ataaaatca
//
