LOCUS       sequence-276438-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP16 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP16
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
       61 gccaactttg tacaaaaaag ttggcatgtc ttcccaggct tggcagcctg gagtggctat
      121 gcctaacctg tacaagatgc agcgtatgct gctggagaag tgcgacctgc agaactacgg
      181 agactccgcc accctgccca agggtatcat gatgaacgtg gctaagtaca cccagctgtg
      241 ccagtacctg aacaccctga ccctggccgt gccttacaac atgcgcgtga tccacttcgg
      301 tgctggttct gacaagggag tggctccagg taccgctgtg ctgagacagt ggctgcccac
      361 cggtaccctg ctggtggact ctgacctgaa cgacttcgtg tctgatgctg acagcaccct
      421 gatcggcgac tgtgctactg tgcacaccgc taacaagtgg gacctgatca tctctgacat
      481 gtacgaccca aagaccaaga acgtgaccaa ggagaacgac agcaaggaag gtttcttcac
      541 ctacatctgc ggcttcatcc agcagaagct ggccctgggc ggatctgtgg ctatcaagat
      601 caccgaacac tcctggaacg ctgacctgta caagctgatg ggacacttcg cctggtggac
      661 cgctttcgtg accaacgtga acgcctctag ctccgaggct ttcctgatcg gctgcaacta
      721 cctgggaaag ccccgtgaac agatcgacgg ttacgtgatg cacgccaact acatcttctg
      781 gcgcaacacc aaccctatcc agctgtctag ctactccctg ttcgacatgt ctaagttccc
      841 actgaagctg agaggcaccg ctgtgatgag cctgaaagga
//
