LOCUS       sequence-276439-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP16 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP16
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgacctgtt cgttgcaaca aattgataag caatgctttc ttataatgcc aactttgtac
       61 aagaaagttg ggtattagtt gttcaccagc acgtcagagg agatgaccac tctgttgttt
      121 tccctgatga tcaggcgtcc cttggacagc aggctcagga tcatgtcgtt gatctgtccc
      181 tccttcaggc tcatcacagc ggtgcctctc agcttcagtg ggaacttaga catgtcgaac
      241 agggagtagc tagacagctg gatagggttg gtgttgcgcc agaagatgta gttggcgtgc
      301 atcacgtaac cgtcgatctg ttcacggggc tttcccaggt agttgcagcc gatcaggaaa
      361 gcctcggagc tagaggcgtt cacgttggtc acgaaagcgg tccaccaggc gaagtgtccc
      421 atcagcttgt acaggtcagc gttccaggag tgttcggtga tcttgatagc cacagatccg
      481 cccagggcca gcttctgctg gatgaagccg cagatgtagg tgaagaaacc ttccttgctg
      541 tcgttctcct tggtcacgtt cttggtcttt gggtcgtaca tgtcagagat gatcaggtcc
      601 cacttgttag cggtgtgcac agtagcacag tcgccgatca gggtgctgtc agcatcagac
      661 acgaagtcgt tcaggtcaga gtccaccagc agggtaccgg tgggcagcca ctgtctcagc
      721 acagcggtac ctggagccac tcccttgtca gaaccagcac cgaagtggat cacgcgcatg
      781 ttgtaaggca cggccagggt cagggtgttc aggtactggc acagctgggt gtacttagcc
      841 acgttcatca tgataccctt gggcagggtg gcggag
//
